Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997989260> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W1997989260 endingPage "1877" @default.
- W1997989260 startingPage "1874" @default.
- W1997989260 abstract "A biosensor based on pyruvate oxidase (POX) enzyme was developed for the investigation of the effect of thiamine (vitamin B1) molecule on the activity of the enzyme. The biosensor was prepared with a chemical covalent immobilization method on the dissolved oxygen (DO) probe by using gelatin and cross-linking agent, glutaraldehyde. POX catalyzes the degradation of pyruvate to acetylphosphate, CO2 and H2O2 in the presence of phosphate and oxygen. Thiamine is an activator for POX enzyme and determination method of the biosensor was based on this effect of thiamine on the activity of the enzyme. The biosensor responses showed increases in the presence of thiamine. Increases in the biosensor responses were related to thiamine concentration. Thiamine determination is based on the assay of the differences on the biosensor responses on the oxygenmeter in the absence and the presence of thiamine. The biosensor response depend linearly on thiamine concentration between 0.025 and 0.5 μM with 2 min response time. In the optimization studies of the biosensor the most suitable enzyme amount was found as 2.5 U cm−2 and also phosphate buffer (pH 7.0; 50 mM) and 35 °C were obtained as the optimum working conditions. In the characterization studies of the biosensor some parameters such as activator and interference effects of some substances on the biosensor response and reproducibility were carried out." @default.
- W1997989260 created "2016-06-24" @default.
- W1997989260 creator A5000173549 @default.
- W1997989260 creator A5034327765 @default.
- W1997989260 date "2008-07-01" @default.
- W1997989260 modified "2023-09-26" @default.
- W1997989260 title "A novel biosensor based on activation effect of thiamine on the activity of pyruvate oxidase" @default.
- W1997989260 cites W126869179 @default.
- W1997989260 cites W1502313760 @default.
- W1997989260 cites W1550287933 @default.
- W1997989260 cites W1968428725 @default.
- W1997989260 cites W1978501410 @default.
- W1997989260 cites W1980501030 @default.
- W1997989260 cites W1981947870 @default.
- W1997989260 cites W1984167957 @default.
- W1997989260 cites W1995663025 @default.
- W1997989260 cites W1996531094 @default.
- W1997989260 cites W1998341894 @default.
- W1997989260 cites W2001383140 @default.
- W1997989260 cites W2003559677 @default.
- W1997989260 cites W2006766324 @default.
- W1997989260 cites W2006778123 @default.
- W1997989260 cites W2012379989 @default.
- W1997989260 cites W2012710118 @default.
- W1997989260 cites W2023487368 @default.
- W1997989260 cites W2029810807 @default.
- W1997989260 cites W2035121898 @default.
- W1997989260 cites W2046283112 @default.
- W1997989260 cites W2053558827 @default.
- W1997989260 cites W2062238557 @default.
- W1997989260 cites W2065380402 @default.
- W1997989260 cites W2068156590 @default.
- W1997989260 cites W2077933546 @default.
- W1997989260 cites W2091141690 @default.
- W1997989260 cites W2092994511 @default.
- W1997989260 cites W2128148402 @default.
- W1997989260 cites W2128366155 @default.
- W1997989260 cites W2157099914 @default.
- W1997989260 cites W2172024862 @default.
- W1997989260 doi "https://doi.org/10.1016/j.bios.2008.03.001" @default.
- W1997989260 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18430561" @default.
- W1997989260 hasPublicationYear "2008" @default.
- W1997989260 type Work @default.
- W1997989260 sameAs 1997989260 @default.
- W1997989260 citedByCount "25" @default.
- W1997989260 countsByYear W19979892602012 @default.
- W1997989260 countsByYear W19979892602013 @default.
- W1997989260 countsByYear W19979892602014 @default.
- W1997989260 countsByYear W19979892602015 @default.
- W1997989260 countsByYear W19979892602016 @default.
- W1997989260 countsByYear W19979892602017 @default.
- W1997989260 countsByYear W19979892602019 @default.
- W1997989260 countsByYear W19979892602020 @default.
- W1997989260 countsByYear W19979892602022 @default.
- W1997989260 crossrefType "journal-article" @default.
- W1997989260 hasAuthorship W1997989260A5000173549 @default.
- W1997989260 hasAuthorship W1997989260A5034327765 @default.
- W1997989260 hasConcept C103432212 @default.
- W1997989260 hasConcept C160756335 @default.
- W1997989260 hasConcept C181199279 @default.
- W1997989260 hasConcept C185592680 @default.
- W1997989260 hasConcept C197957613 @default.
- W1997989260 hasConcept C2776203871 @default.
- W1997989260 hasConcept C2777529624 @default.
- W1997989260 hasConcept C2780047943 @default.
- W1997989260 hasConcept C43617362 @default.
- W1997989260 hasConcept C55493867 @default.
- W1997989260 hasConceptScore W1997989260C103432212 @default.
- W1997989260 hasConceptScore W1997989260C160756335 @default.
- W1997989260 hasConceptScore W1997989260C181199279 @default.
- W1997989260 hasConceptScore W1997989260C185592680 @default.
- W1997989260 hasConceptScore W1997989260C197957613 @default.
- W1997989260 hasConceptScore W1997989260C2776203871 @default.
- W1997989260 hasConceptScore W1997989260C2777529624 @default.
- W1997989260 hasConceptScore W1997989260C2780047943 @default.
- W1997989260 hasConceptScore W1997989260C43617362 @default.
- W1997989260 hasConceptScore W1997989260C55493867 @default.
- W1997989260 hasIssue "12" @default.
- W1997989260 hasLocation W19979892601 @default.
- W1997989260 hasLocation W19979892602 @default.
- W1997989260 hasOpenAccess W1997989260 @default.
- W1997989260 hasPrimaryLocation W19979892601 @default.
- W1997989260 hasRelatedWork W1971308409 @default.
- W1997989260 hasRelatedWork W1988856018 @default.
- W1997989260 hasRelatedWork W1997989260 @default.
- W1997989260 hasRelatedWork W2014089666 @default.
- W1997989260 hasRelatedWork W2040106523 @default.
- W1997989260 hasRelatedWork W2082301358 @default.
- W1997989260 hasRelatedWork W2095419453 @default.
- W1997989260 hasRelatedWork W2208020336 @default.
- W1997989260 hasRelatedWork W2407728773 @default.
- W1997989260 hasRelatedWork W2806378807 @default.
- W1997989260 hasVolume "23" @default.
- W1997989260 isParatext "false" @default.
- W1997989260 isRetracted "false" @default.
- W1997989260 magId "1997989260" @default.
- W1997989260 workType "article" @default.