Matches in SemOpenAlex for { <https://semopenalex.org/work/W1997991619> ?p ?o ?g. }
- W1997991619 endingPage "137" @default.
- W1997991619 startingPage "129" @default.
- W1997991619 abstract "The bipartite Abutilon mosaic virus (AbMV) was engineered as a versatile silencing vector in which the coat protein gene of DNA A was deleted and replaced by sequences of interest. Plants transgenic for the dimeric AbMV DNA B component were used as test hosts to minimize the risk of unintended release of the recombinant DNA. The vector construct was stable genetically upon systemic infection and, in common with the parental virus, the vector remained phloem-limited. For virus-induced gene silencing (VIGS), a phytoene desaturase gene fragment was isolated from Nicotiana benthamiana (NbPDS) and inserted into the vector. After agroinfection, phytoene desaturase silencing was triggered efficiently in all leaf tissues without interference by viral symptoms. In order to facilitate further the use of the system, a technique for cell-free construction of recombinants was established using rolling circle amplification and biolistic inoculation of DNA B-transgenic plants. This novel procedure provides a convenient and safe way for delivering VIGS constructs for functional genomics." @default.
- W1997991619 created "2016-06-24" @default.
- W1997991619 creator A5007439946 @default.
- W1997991619 creator A5037707570 @default.
- W1997991619 creator A5089791250 @default.
- W1997991619 date "2010-10-01" @default.
- W1997991619 modified "2023-10-16" @default.
- W1997991619 title "Cell-free construction of disarmed Abutilon mosaic virus-based gene silencing vectors" @default.
- W1997991619 cites W1489746883 @default.
- W1997991619 cites W1540414292 @default.
- W1997991619 cites W1964258737 @default.
- W1997991619 cites W1964624783 @default.
- W1997991619 cites W1971941597 @default.
- W1997991619 cites W1976266580 @default.
- W1997991619 cites W1978542189 @default.
- W1997991619 cites W1981999151 @default.
- W1997991619 cites W1988800991 @default.
- W1997991619 cites W1991880907 @default.
- W1997991619 cites W1994065931 @default.
- W1997991619 cites W1994717043 @default.
- W1997991619 cites W1997767376 @default.
- W1997991619 cites W2005459122 @default.
- W1997991619 cites W2007710834 @default.
- W1997991619 cites W2008411508 @default.
- W1997991619 cites W2014623895 @default.
- W1997991619 cites W2018183355 @default.
- W1997991619 cites W2024219875 @default.
- W1997991619 cites W2026073168 @default.
- W1997991619 cites W2028030581 @default.
- W1997991619 cites W2031160222 @default.
- W1997991619 cites W2031448361 @default.
- W1997991619 cites W2031924344 @default.
- W1997991619 cites W2034041196 @default.
- W1997991619 cites W2037104056 @default.
- W1997991619 cites W2040834075 @default.
- W1997991619 cites W2045089252 @default.
- W1997991619 cites W2046912447 @default.
- W1997991619 cites W2047439748 @default.
- W1997991619 cites W2055737305 @default.
- W1997991619 cites W2057038789 @default.
- W1997991619 cites W2057845428 @default.
- W1997991619 cites W2059383645 @default.
- W1997991619 cites W2059421576 @default.
- W1997991619 cites W2063360408 @default.
- W1997991619 cites W2063376320 @default.
- W1997991619 cites W2068066258 @default.
- W1997991619 cites W2075031558 @default.
- W1997991619 cites W2076253788 @default.
- W1997991619 cites W2081579270 @default.
- W1997991619 cites W2082768531 @default.
- W1997991619 cites W2084927785 @default.
- W1997991619 cites W2094628735 @default.
- W1997991619 cites W2103232352 @default.
- W1997991619 cites W2105299972 @default.
- W1997991619 cites W2113917808 @default.
- W1997991619 cites W2126611555 @default.
- W1997991619 cites W2133227619 @default.
- W1997991619 cites W2134681297 @default.
- W1997991619 cites W2138830644 @default.
- W1997991619 cites W2138835651 @default.
- W1997991619 cites W2141573080 @default.
- W1997991619 cites W2143014336 @default.
- W1997991619 cites W2149093485 @default.
- W1997991619 cites W2152294576 @default.
- W1997991619 cites W2152591200 @default.
- W1997991619 cites W2168097332 @default.
- W1997991619 cites W2169151775 @default.
- W1997991619 cites W2171260128 @default.
- W1997991619 cites W2176605931 @default.
- W1997991619 cites W2407575497 @default.
- W1997991619 doi "https://doi.org/10.1016/j.jviromet.2010.07.010" @default.
- W1997991619 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20638413" @default.
- W1997991619 hasPublicationYear "2010" @default.
- W1997991619 type Work @default.
- W1997991619 sameAs 1997991619 @default.
- W1997991619 citedByCount "20" @default.
- W1997991619 countsByYear W19979916192012 @default.
- W1997991619 countsByYear W19979916192013 @default.
- W1997991619 countsByYear W19979916192014 @default.
- W1997991619 countsByYear W19979916192015 @default.
- W1997991619 countsByYear W19979916192017 @default.
- W1997991619 countsByYear W19979916192018 @default.
- W1997991619 countsByYear W19979916192019 @default.
- W1997991619 countsByYear W19979916192021 @default.
- W1997991619 countsByYear W19979916192023 @default.
- W1997991619 crossrefType "journal-article" @default.
- W1997991619 hasAuthorship W1997991619A5007439946 @default.
- W1997991619 hasAuthorship W1997991619A5037707570 @default.
- W1997991619 hasAuthorship W1997991619A5089791250 @default.
- W1997991619 hasConcept C104317684 @default.
- W1997991619 hasConcept C109110057 @default.
- W1997991619 hasConcept C119056186 @default.
- W1997991619 hasConcept C141231307 @default.
- W1997991619 hasConcept C159047783 @default.
- W1997991619 hasConcept C161078062 @default.
- W1997991619 hasConcept C166703698 @default.
- W1997991619 hasConcept C189206191 @default.
- W1997991619 hasConcept C2522874641 @default.