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- W1997997658 abstract "An instrument was fabricated to measure the real-time response of a bioluminescent construct of Escherichia coli to ozone treatment. The bioluminescent output from E. coli inoculated on nutrient agar plates was measured with a photomultiplier tube coupled to a lock-in amplifier and the ozone concentration was calculated from absorption measurements from a 254 nm source. Data from the two measurement systems were acquired in real-time via an oscilloscope, captured to a PC for analysis and displayed on the graphical user interface. The ozone and bioluminescence measurements were done in two different chambers to isolate the measurement systems and to prevent any adverse effects of the UV radiation on the bacteria. The real-time data showed the instantaneous changes in the bioluminescence output with increasing ozone concentration. The dark current from plastic petri dishes both with and without agar was measured and a peak to peak variation of 0.10 dB in the signal was observed; this was negligible compared to the bioluminescent signal. A real time monitoring system for microbial viability would be of relevance for pathogenic and spoilage microorganisms as well as probiotics. This paper demonstrates the potential to use a bioluminiscent microorganism to evaluate the effectiveness of decontamination systems and possibly leading to their optimization." @default.
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- W1997997658 date "2005-06-01" @default.
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- W1997997658 title "A real-time monitoring and detection instrument for analysis of the effects of O3 on bioluminescent Escherichia coli on agar surfaces—potential applications to the food industry" @default.
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- W1997997658 doi "https://doi.org/10.1016/j.ifset.2004.12.003" @default.
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