FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1998007473> ?p ?o ?g. }

• W1998007473 endingPage "e64211" @default.
• W1998007473 startingPage "e64211" @default.
• W1998007473 abstract "Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 is a major cause of zoonotic food- and water-borne intestinal infections worldwide with clinical consequences ranging from mild diarrhoea to hemolytic uraemic syndrome. The genome of EHEC O157:H7 contains many regions of unique DNA that are referred to as O islands including the Shiga toxin prophages and pathogenicity islands encoding key virulence factors. However many of these O islands are of unknown function. In this study, genetic analysis was conducted on OI-172 which is a 44,434 bp genomic island with 27 open reading frames. Comparative genome analysis showed that O1-72 is a composite island with progressive gain of genes since O157:H7 evolved from its ancestral O55:H7. A partial OI-172 island was also found in 2 unrelated E. coli strains and 2 Salmonella strains. OI-172 encodes several putative helicases, one of which (Z5898) is a putative DEAH box RNA helicase. To investigate the function of Z5898, a deletion mutant (EDL933ΔZ5898) was constructed in the O157:H7 strain EDL933. Comparative proteomic analysis of the mutant with the wild-type EDL933 found that flagellin was down-regulated in the Z5898 mutant. Motility assay showed that EDL933ΔZ5898 migrated slower than the wild-type EDL933 and electron microscopy found no surface flagella. Quantitative reverse transcription PCR revealed that the fliC expression of EDL933ΔZ5898 was significantly lower while the expression of its upstream regulator gene, fliA, was not affected. Using a fliA and a fliC promoter – green fluorescent protein fusion contruct, Z5898 was found to affect only the fliC promoter activity. Therefore, Z5898 regulates the flagella based motility by exerting its effect on fliC. We conclude that OI-172 is a motility associated O island and hereby name it the MAO island." @default.
• W1998007473 created "2016-06-24" @default.
• W1998007473 creator A5005283872 @default.
• W1998007473 creator A5005909676 @default.
• W1998007473 creator A5016083247 @default.
• W1998007473 creator A5017114509 @default.
• W1998007473 creator A5040443849 @default.
• W1998007473 creator A5042274161 @default.
• W1998007473 creator A5063481044 @default.
• W1998007473 creator A5064998305 @default.
• W1998007473 creator A5071531284 @default.
• W1998007473 creator A5072502783 @default.
• W1998007473 date "2013-06-13" @default.
• W1998007473 modified "2023-10-12" @default.
• W1998007473 title "An O Island 172 Encoded RNA Helicase Regulates the Motility of Escherichia coli O157:H7" @default.
• W1998007473 cites W1886048099 @default.
• W1998007473 cites W1913315326 @default.
• W1998007473 cites W1937273107 @default.
• W1998007473 cites W1941337723 @default.
• W1998007473 cites W1972527322 @default.
• W1998007473 cites W1974074108 @default.
• W1998007473 cites W1975497884 @default.
• W1998007473 cites W1985396638 @default.
• W1998007473 cites W2011383767 @default.
• W1998007473 cites W2025605962 @default.
• W1998007473 cites W2026038510 @default.
• W1998007473 cites W2030426089 @default.
• W1998007473 cites W2032606946 @default.
• W1998007473 cites W2038916450 @default.
• W1998007473 cites W2042482682 @default.
• W1998007473 cites W2045765967 @default.
• W1998007473 cites W2049577391 @default.
• W1998007473 cites W2050002984 @default.
• W1998007473 cites W2050946340 @default.
• W1998007473 cites W2051449352 @default.
• W1998007473 cites W2057474502 @default.
• W1998007473 cites W2065926204 @default.
• W1998007473 cites W2067127765 @default.
• W1998007473 cites W2084328564 @default.
• W1998007473 cites W2089009257 @default.
• W1998007473 cites W2091708538 @default.
• W1998007473 cites W2101058428 @default.
• W1998007473 cites W2102545648 @default.
• W1998007473 cites W2103016908 @default.
• W1998007473 cites W2103113934 @default.
• W1998007473 cites W2106487742 @default.
• W1998007473 cites W2107277218 @default.
• W1998007473 cites W2110655080 @default.
• W1998007473 cites W2112186461 @default.
• W1998007473 cites W2112864683 @default.
• W1998007473 cites W2125153220 @default.
• W1998007473 cites W2131242123 @default.
• W1998007473 cites W2137055458 @default.
• W1998007473 cites W2141245000 @default.
• W1998007473 cites W2142572552 @default.
• W1998007473 cites W2143446377 @default.
• W1998007473 cites W2143452405 @default.
• W1998007473 cites W2146706147 @default.
• W1998007473 cites W2151100831 @default.
• W1998007473 cites W2151315479 @default.
• W1998007473 cites W2152146866 @default.
• W1998007473 cites W2156602474 @default.
• W1998007473 cites W2160847617 @default.
• W1998007473 cites W2161744554 @default.
• W1998007473 cites W2161782181 @default.
• W1998007473 cites W2163089618 @default.
• W1998007473 cites W2166802829 @default.
• W1998007473 cites W2167887447 @default.
• W1998007473 cites W2169231066 @default.
• W1998007473 cites W2170698461 @default.
• W1998007473 cites W2208675311 @default.
• W1998007473 doi "https://doi.org/10.1371/journal.pone.0064211" @default.
• W1998007473 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3681947" @default.
• W1998007473 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23785398" @default.
• W1998007473 hasPublicationYear "2013" @default.
• W1998007473 type Work @default.
• W1998007473 sameAs 1998007473 @default.
• W1998007473 citedByCount "42" @default.
• W1998007473 countsByYear W19980074732015 @default.
• W1998007473 countsByYear W19980074732016 @default.
• W1998007473 countsByYear W19980074732017 @default.
• W1998007473 countsByYear W19980074732018 @default.
• W1998007473 countsByYear W19980074732019 @default.
• W1998007473 countsByYear W19980074732020 @default.
• W1998007473 countsByYear W19980074732021 @default.
• W1998007473 countsByYear W19980074732022 @default.
• W1998007473 countsByYear W19980074732023 @default.
• W1998007473 crossrefType "journal-article" @default.
• W1998007473 hasAuthorship W1998007473A5005283872 @default.
• W1998007473 hasAuthorship W1998007473A5005909676 @default.
• W1998007473 hasAuthorship W1998007473A5016083247 @default.
• W1998007473 hasAuthorship W1998007473A5017114509 @default.
• W1998007473 hasAuthorship W1998007473A5040443849 @default.
• W1998007473 hasAuthorship W1998007473A5042274161 @default.
• W1998007473 hasAuthorship W1998007473A5063481044 @default.
• W1998007473 hasAuthorship W1998007473A5064998305 @default.
• W1998007473 hasAuthorship W1998007473A5071531284 @default.
• W1998007473 hasAuthorship W1998007473A5072502783 @default.

• next