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- W1998045154 abstract "Human HeLa cells were fused with Haplopappus gracilis protoplasts by using inactivated Sendai virus as a fusion agent. Haplopappus gracilis is a species of Compositae with two chromosomes (n = 2). After fusion, the cell components were cultured in human culture medium (modified MEM) and maintained at 37°C. Before fusion, H3-thymidine and H3-uridine were added to label the nucleus and the cytoplasm of the human cells. The human and Haplopappus nuclei, as well as their cytoplasms, could be distinguished by means of carbol-fuchsin staining and by the autoradiographic procedure. Three days after fusion the following was observed: (1) The Haplopappus protoplasts which had not fused had rebuilt their cell wall and were virtually indistinguishable from control cells growing at 25°C and in plant culture medium. (2) Cell fusion between human and plant cells had led to the formation of heterokaryons containing one human and one Haplopappus nucleus which appeared with a frequency of 12%. (3) In the heterokaryons a cell wall had formed which surrounded the two types of cytoplasms that had become mixed. (4) Heterokaryons with several HeLa and Haplopappus nuclei were also present in the preparations. The fusion of human cells with plant protoplasts has several implications in connection with the basic organization of the eukaryotic chromosome." @default.
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- W1998045154 date "2009-02-12" @default.
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- W1998045154 title "Fusion of human cells with Haplopappus protoplasts by means of Sendai virus" @default.
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- W1998045154 doi "https://doi.org/10.1111/j.1601-5223.1977.tb01246.x" @default.
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