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• W1998049504 abstract "A micellar electrokinetic capillary chromatography (MECC) method has been developed for the determination of the four isoflavones, i.e. biochanin A, formononetin, genstein and daidzein in red clover (Trifolium Pratense L.). The effect of running buffer pH and concentration were investigated. An electrolyte composed of 30 mm borate, 20 mm sodium dodecyl sulfate (SDS) and 4 mg/mL HP-β-CD containing 5% (v/v) ethanol at pH 10.1 provides a satisfactory separation for all the analytes. The applied voltage was 25 kV, and the capillary temperature was kept constant at 25°C with a UV detection at 254 nm. The relative standard deviations (RSD) of the migration time and peak area were less than 1.73 and 3.94% (intra-day), and 2.29 and 4.38% (inter-day), respectively, under the optimized separation conditions. Regression equations revealed a good linear relationship between the peak area of each compound and its concentration. The contents of the four compounds in red clover were successfully determined with satisfactory repeatability and recovery. Copyright © 2007 John Wiley & Sons, Ltd." @default.
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• W1998049504 date "2007-01-01" @default.
• W1998049504 modified "2023-09-26" @default.
• W1998049504 title "Separation and determination of isoflavones in red clover by micellar electrokinetic capillary chromatography" @default.
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• W1998049504 doi "https://doi.org/10.1002/bmc.843" @default.
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