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- W1998064688 abstract "Molecular subtyping is of significant importance to the recognition of outbreaks of meningococcal disease caused by serogroup C Neisseria meningitidis. We describe the application of multilocus variable number tandem repeat analysis (MLVA) for the molecular subtyping of N. meningitidis and compare its performance to that of pulsed-field gel electrophoresis (PFGE). For MLVA, a multiplex PCR assay targeting five variable number tandem repeat regions was developed and evaluated using a panel of sporadic and outbreak-associated serogroup C N. meningitidis isolates. MLVA was highly reproducible and provided results within 6 h. Overall, the discriminatory power of MLVA was equivalent to that of PFGE. The utilization of MLVA for subtyping N. meningitidis isolates provides a rapid and safer alternative to PFGE for identifying outbreaks of meningococcal disease. As such, it may provide public health officials with timely information that may minimize the spread of outbreak-related cases through prophylaxis." @default.
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- W1998064688 date "2006-11-01" @default.
- W1998064688 modified "2023-09-26" @default.
- W1998064688 title "Rapid laboratory detection of meningococcal disease outbreaks caused by serogroup C Neisseria meningitidis" @default.
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- W1998064688 doi "https://doi.org/10.1016/j.mimet.2006.04.004" @default.
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