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• W1998096945 abstract "We have proposed previously that error‐prone reverse transcription using pre‐mRNA of rearranged immunoglobulin variable (IgV) regions as templates is involved in the antibody diversifying mechanism of somatic hypermutation (SHM). As patients deficient in DNA polymerase‐η exhibit an abnormal spectrum of SHM, we postulated that this recently discovered Y‐family polymerase is a reverse transcriptase (RT). This possibility was tested using a product‐enhanced RT (PERT) assay that uses a real time PCR step with a fluorescent probe to detect cDNA products of at least 27−37 nucleotides. Human pol‐η and two other Y‐family enzymes that are dispensable for SHM, human pols‐ι and ‐κ, copied a heteropolymeric DNA‐primed RNA template in vitro under conditions with substantial excesses of template. Repeated experiments gave highly reproducible results. The RT activity detected using one aliquot of human pol‐η was confirmed using a second sample from an independent source. Human DNA pols‐β and ‐µ, and T4 DNA polymerase repeatedly demonstrated no RT activity. Pol‐η was the most efficient RT of the Y‐family enzymes assayed but was much less efficient than an HIV‐RT standard in vitro . It is thus feasible that pol‐η acts as both a RNA‐ and a DNA‐dependent DNA polymerase in SHM in vivo , and that Y‐family RT activity participates in other mechanisms of physiological importance." @default.
• W1998096945 created "2016-06-24" @default.
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• W1998096945 creator A5075370774 @default.
• W1998096945 date "2004-04-01" @default.
• W1998096945 modified "2023-09-26" @default.
• W1998096945 title "Human DNA polymerase‐η, an A‐T mutator in somatic hypermutation of rearranged immunoglobulin genes, is a reverse transcriptase" @default.
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• W1998096945 doi "https://doi.org/10.1046/j.0818-9641.2004.01221.x" @default.
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