FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1998117510> ?p ?o ?g. }

• W1998117510 endingPage "498" @default.
• W1998117510 startingPage "488" @default.
• W1998117510 abstract "Summary PEX7 is a soluble import receptor that recognizes peroxisomal targeting signal type 2 (PTS2)‐containing proteins. In the present study, using a green fluorescent protein (GFP) fusion protein of PEX7 (GFP‐PEX7), we analyzed the molecular function and subcellular localization of PEX7 in Arabidopsis thaliana . The overexpression of GFP‐PEX7 resulted in defective glyoxysomal fatty acid β‐oxidation, but had no significant effect on leaf peroxisomal function. Analysis of the subcellular localization of GFP‐PEX7 in transgenic Arabidopsis showed that GFP‐PEX7 localizes primarily to the peroxisome. Transient expression of a C‐ or N‐terminal fusion protein of PEX7 and yellow fluorescent protein (YFP) (PEX7‐YFP and YFP‐PEX7, respectively) in leek epidermal cells, using the particle bombardment technique, confirmed that fluorescent protein‐tagged PEX7 localizes to peroxisomes in Arabidopsis. Immunoblot analysis revealed that GFP‐PEX7 accumulates primarily in peroxisomal membrane fractions, whereas endogenous PEX7 was distributed evenly in cytosolic and peroxisomal membrane fractions, which indicated that both endogenous PEX7 and GFP‐PEX7 are properly targeted to peroxisomal membranes. The results of bimolecular fluorescence complementation (BiFC) and yeast two‐hybrid analyses showed that PEX7 binds directly to PTS2‐containing proteins and PEX12 in the peroxisomal membrane. We used red fluorescent protein (tdTomato) fusion protein of PEX7 (tdTomato‐PEX7) in several Arabidopsis pex mutants to identify proteins required for the targeting of PEX7 to peroxisomes in planta . The results demonstrated that pex14 , pex13 and pex12 mutations disrupt the proper targeting of PEX7 to peroxisomes. Overall, our results suggest that the targeting of PEX7 to peroxisomes requires four proteins: a PTS2‐containing protein, PEX14, PEX13 and PEX12." @default.
• W1998117510 created "2016-06-24" @default.
• W1998117510 creator A5010430492 @default.
• W1998117510 creator A5011693386 @default.
• W1998117510 creator A5021033335 @default.
• W1998117510 creator A5028256389 @default.
• W1998117510 creator A5053848574 @default.
• W1998117510 creator A5082850281 @default.
• W1998117510 date "2009-10-28" @default.
• W1998117510 modified "2023-10-15" @default.
• W1998117510 title "Molecular components required for the targeting of PEX7 to peroxisomes in<i>Arabidopsis thaliana</i>" @default.
• W1998117510 cites W1579834701 @default.
• W1998117510 cites W1719904978 @default.
• W1998117510 cites W1903792504 @default.
• W1998117510 cites W1977186059 @default.
• W1998117510 cites W1980400357 @default.
• W1998117510 cites W1983112089 @default.
• W1998117510 cites W1983281364 @default.
• W1998117510 cites W1983928246 @default.
• W1998117510 cites W1986327432 @default.
• W1998117510 cites W1996385741 @default.
• W1998117510 cites W2009917506 @default.
• W1998117510 cites W2010813706 @default.
• W1998117510 cites W2014492708 @default.
• W1998117510 cites W2016619307 @default.
• W1998117510 cites W2018494620 @default.
• W1998117510 cites W2036388592 @default.
• W1998117510 cites W2043401246 @default.
• W1998117510 cites W2045328094 @default.
• W1998117510 cites W2047439787 @default.
• W1998117510 cites W2047561285 @default.
• W1998117510 cites W2049234265 @default.
• W1998117510 cites W2061217564 @default.
• W1998117510 cites W2061712981 @default.
• W1998117510 cites W2061772847 @default.
• W1998117510 cites W2074597358 @default.
• W1998117510 cites W2079614823 @default.
• W1998117510 cites W2080114061 @default.
• W1998117510 cites W2081283882 @default.
• W1998117510 cites W2088087668 @default.
• W1998117510 cites W2090180566 @default.
• W1998117510 cites W2099786454 @default.
• W1998117510 cites W2099876280 @default.
• W1998117510 cites W2105860639 @default.
• W1998117510 cites W2114019156 @default.
• W1998117510 cites W2115027228 @default.
• W1998117510 cites W2115734610 @default.
• W1998117510 cites W2119760234 @default.
• W1998117510 cites W2121077965 @default.
• W1998117510 cites W2122941138 @default.
• W1998117510 cites W2123058210 @default.
• W1998117510 cites W2130310848 @default.
• W1998117510 cites W2132762612 @default.
• W1998117510 cites W2132879645 @default.
• W1998117510 cites W2133963159 @default.
• W1998117510 cites W2137938732 @default.
• W1998117510 cites W2138526665 @default.
• W1998117510 cites W2142294740 @default.
• W1998117510 cites W2142883760 @default.
• W1998117510 cites W2146986282 @default.
• W1998117510 cites W2149018336 @default.
• W1998117510 cites W2151956233 @default.
• W1998117510 cites W2157909952 @default.
• W1998117510 cites W2159803276 @default.
• W1998117510 cites W2161223616 @default.
• W1998117510 cites W2165823564 @default.
• W1998117510 cites W4229864359 @default.
• W1998117510 cites W4232586824 @default.
• W1998117510 doi "https://doi.org/10.1111/j.1365-313x.2009.03970.x" @default.
• W1998117510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19594707" @default.
• W1998117510 hasPublicationYear "2009" @default.
• W1998117510 type Work @default.
• W1998117510 sameAs 1998117510 @default.
• W1998117510 citedByCount "26" @default.
• W1998117510 countsByYear W19981175102012 @default.
• W1998117510 countsByYear W19981175102013 @default.
• W1998117510 countsByYear W19981175102014 @default.
• W1998117510 countsByYear W19981175102015 @default.
• W1998117510 countsByYear W19981175102016 @default.
• W1998117510 countsByYear W19981175102017 @default.
• W1998117510 countsByYear W19981175102018 @default.
• W1998117510 countsByYear W19981175102019 @default.
• W1998117510 countsByYear W19981175102020 @default.
• W1998117510 countsByYear W19981175102022 @default.
• W1998117510 crossrefType "journal-article" @default.
• W1998117510 hasAuthorship W1998117510A5010430492 @default.
• W1998117510 hasAuthorship W1998117510A5011693386 @default.
• W1998117510 hasAuthorship W1998117510A5021033335 @default.
• W1998117510 hasAuthorship W1998117510A5028256389 @default.
• W1998117510 hasAuthorship W1998117510A5053848574 @default.
• W1998117510 hasAuthorship W1998117510A5082850281 @default.
• W1998117510 hasBestOaLocation W19981175101 @default.
• W1998117510 hasConcept C104317684 @default.
• W1998117510 hasConcept C123243835 @default.
• W1998117510 hasConcept C123894998 @default.
• W1998117510 hasConcept C127078168 @default.
• W1998117510 hasConcept C142613039 @default.
• W1998117510 hasConcept C143065580 @default.

• next