FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1998202459> ?p ?o ?g. }

• W1998202459 endingPage "747" @default.
• W1998202459 startingPage "739" @default.
• W1998202459 abstract "Pyrrole‐2‐carboxylate can serve as the sole source of carbon, nitrogen, and energy for a strain tentatively identified to belong to the genus Rhodococcus. An NADH‐dependent oxygenase activity was detected in cell extracts that initiated the degradation of the substrate. During purification of the enzyme, this activity was separated into two protein components which were both purified to apparent homogeneity. A small monomeric 18.7‐kDa protein designated as reductase, catalyzed in vitro the NADH and FAD‐dependent reduction of cytochrome c and had an NADH‐oxidase activity. The second component, a 54‐kDa protein with a trimeric native structure had no enzymatic activity by itself, but exhibited a pyrrole‐2‐carboxylate‐dependent oxygen consumption when it was complemented with the reductase component, FAD, and NADH. This indicated that the large protein referred to as oxygenase was responsible for the oxygen‐dependent hydroxylation of the substrate. The rate of an uncoupled NADH oxidation without hydroxylation of the substrate was found to be strongly dependent on the molar ratio of both components. The uncoupling was nearly completely suppressed by a 5–7‐fold molar excess of the oxygenase component. The small protein was N‐terminally blocked. It was thus proteolytically digested and four of the resulting peptides were sequenced comprising 47 amino acids. The sequences of these fragments were similar to the sequences reported for the small component of different two‐component flavin monooxygenases. Furthermore, the N‐terminus of the oxygenase component showed high sequence similarity to the second, usually large subunit of these enzymes and to two single‐component flavin monooxygenases. Thus, the enzyme from Rhodococcus sp. designated as pyrrole‐2‐carboxylate monooxygenase belongs to the recently discovered new class of two‐component flavin aromatic monooxygenases. Some of the basic properties of both components were determined and their interaction during catalysis was investigated." @default.
• W1998202459 created "2016-06-24" @default.
• W1998202459 creator A5007187482 @default.
• W1998202459 creator A5020689553 @default.
• W1998202459 creator A5072078882 @default.
• W1998202459 date "1997-11-01" @default.
• W1998202459 modified "2023-10-16" @default.
• W1998202459 title "Two‐Component Flavin‐dependent Pyrrole‐2‐carboxylate Monooxygenase from <i>Rhodococcus</i> Sp." @default.
• W1998202459 cites W1516064099 @default.
• W1998202459 cites W1523642899 @default.
• W1998202459 cites W1530476407 @default.
• W1998202459 cites W1543349787 @default.
• W1998202459 cites W1565691667 @default.
• W1998202459 cites W1588756347 @default.
• W1998202459 cites W1602201841 @default.
• W1998202459 cites W1780509482 @default.
• W1998202459 cites W1927794787 @default.
• W1998202459 cites W2008000048 @default.
• W1998202459 cites W2047841570 @default.
• W1998202459 cites W2048425637 @default.
• W1998202459 cites W2054446576 @default.
• W1998202459 cites W2070019771 @default.
• W1998202459 cites W2072668027 @default.
• W1998202459 cites W2100571971 @default.
• W1998202459 cites W2107578289 @default.
• W1998202459 cites W2110049624 @default.
• W1998202459 cites W2130925403 @default.
• W1998202459 cites W2136317897 @default.
• W1998202459 cites W2137092948 @default.
• W1998202459 cites W2139484291 @default.
• W1998202459 cites W2141307634 @default.
• W1998202459 cites W2153465764 @default.
• W1998202459 cites W2159702069 @default.
• W1998202459 cites W2171881382 @default.
• W1998202459 cites W2172844043 @default.
• W1998202459 cites W2180551190 @default.
• W1998202459 cites W4255140257 @default.
• W1998202459 cites W4256450827 @default.
• W1998202459 cites W4293247451 @default.
• W1998202459 doi "https://doi.org/10.1111/j.1432-1033.1997.t01-1-00739.x" @default.
• W1998202459 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9395321" @default.
• W1998202459 hasPublicationYear "1997" @default.
• W1998202459 type Work @default.
• W1998202459 sameAs 1998202459 @default.
• W1998202459 citedByCount "31" @default.
• W1998202459 countsByYear W19982024592016 @default.
• W1998202459 countsByYear W19982024592017 @default.
• W1998202459 countsByYear W19982024592018 @default.
• W1998202459 crossrefType "journal-article" @default.
• W1998202459 hasAuthorship W1998202459A5007187482 @default.
• W1998202459 hasAuthorship W1998202459A5020689553 @default.
• W1998202459 hasAuthorship W1998202459A5072078882 @default.
• W1998202459 hasBestOaLocation W19982024591 @default.
• W1998202459 hasConcept C13002179 @default.
• W1998202459 hasConcept C134651460 @default.
• W1998202459 hasConcept C165474305 @default.
• W1998202459 hasConcept C181199279 @default.
• W1998202459 hasConcept C185592680 @default.
• W1998202459 hasConcept C2781109383 @default.
• W1998202459 hasConcept C33093398 @default.
• W1998202459 hasConcept C526171541 @default.
• W1998202459 hasConcept C55493867 @default.
• W1998202459 hasConcept C71240020 @default.
• W1998202459 hasConcept C71995715 @default.
• W1998202459 hasConceptScore W1998202459C13002179 @default.
• W1998202459 hasConceptScore W1998202459C134651460 @default.
• W1998202459 hasConceptScore W1998202459C165474305 @default.
• W1998202459 hasConceptScore W1998202459C181199279 @default.
• W1998202459 hasConceptScore W1998202459C185592680 @default.
• W1998202459 hasConceptScore W1998202459C2781109383 @default.
• W1998202459 hasConceptScore W1998202459C33093398 @default.
• W1998202459 hasConceptScore W1998202459C526171541 @default.
• W1998202459 hasConceptScore W1998202459C55493867 @default.
• W1998202459 hasConceptScore W1998202459C71240020 @default.
• W1998202459 hasConceptScore W1998202459C71995715 @default.
• W1998202459 hasIssue "3" @default.
• W1998202459 hasLocation W19982024591 @default.
• W1998202459 hasLocation W19982024592 @default.
• W1998202459 hasOpenAccess W1998202459 @default.
• W1998202459 hasPrimaryLocation W19982024591 @default.
• W1998202459 hasRelatedWork W1120004047 @default.
• W1998202459 hasRelatedWork W1481111699 @default.
• W1998202459 hasRelatedWork W1993361677 @default.
• W1998202459 hasRelatedWork W2016283620 @default.
• W1998202459 hasRelatedWork W2030566674 @default.
• W1998202459 hasRelatedWork W2032853478 @default.
• W1998202459 hasRelatedWork W2049785072 @default.
• W1998202459 hasRelatedWork W2058812671 @default.
• W1998202459 hasRelatedWork W3116433157 @default.
• W1998202459 hasRelatedWork W646018188 @default.
• W1998202459 hasVolume "249" @default.
• W1998202459 isParatext "false" @default.
• W1998202459 isRetracted "false" @default.
• W1998202459 magId "1998202459" @default.
• W1998202459 workType "article" @default.