Matches in SemOpenAlex for { <https://semopenalex.org/work/W1998204844> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W1998204844 endingPage "251a" @default.
- W1998204844 startingPage "251a" @default.
- W1998204844 abstract "The ubiquitous inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) channel, localized primarily in the ER membrane, releases Ca2+ into the cytoplasm upon binding IP3, generating and modulating intracellular Ca2+ signals that regulate numerous physiological processes. Together with the number of channels activated and the open probability (Po) of the active channels, the size of the unitary Ca2+ current (iCa) passing through an open IP3R channel determines the amount of Ca2+ released from the ER store, and thus the amplitude and the spatial and temporal nature of Ca2+ signals generated in response to extracellular stimuli. Despite its significance, iCa for IP3R channels in physiological ionic conditions has not been directly measured. Here we report the first measurement of iCa through an IP3R channel in its native membrane environment under physiological ionic conditions. Nuclear patch-clamp electrophysiology with rapid perfusion solution exchanges was used to study the conductance properties of recombinant homotetrameric rat type 3 IP3R channels. Within physiological ranges of free Ca2+ concentrations in the ER lumen ([Ca2+]ER), free cytoplasmic [Ca2+] ([Ca2+]i) and symmetric free [Mg2+] ([Mg2+]f), the iCa-[Ca2+]ER relation was linear with no detectable dependence on [Mg2+]f. iCa was 0.15 ± 0.01 pA for a filled ER store with 500 μM [Ca2+]ER. The iCa-[Ca2+]ER relation suggests that Ca2+ released by an IP3R channel raises [Ca2+]i near the open channel to ∼ 13-70 μM, depending on [Ca2+]ER. These measurements have implications for the activities of nearby IP3-liganded IP3R channels, and they confirm that Ca2+ released by an open IP3R channel is sufficient to activate neighboring channels at appropriate distances away, promoting Ca2+-induced Ca2+ release." @default.
- W1998204844 created "2016-06-24" @default.
- W1998204844 creator A5030221375 @default.
- W1998204844 creator A5063587224 @default.
- W1998204844 creator A5071760145 @default.
- W1998204844 date "2011-02-01" @default.
- W1998204844 modified "2023-09-30" @default.
- W1998204844 title "Unitary Calcium Current Through Recombinant Mammalian Type 3 IP3 Receptor Channels Under Physiological Ionic Conditions" @default.
- W1998204844 doi "https://doi.org/10.1016/j.bpj.2010.12.1586" @default.
- W1998204844 hasPublicationYear "2011" @default.
- W1998204844 type Work @default.
- W1998204844 sameAs 1998204844 @default.
- W1998204844 citedByCount "0" @default.
- W1998204844 crossrefType "journal-article" @default.
- W1998204844 hasAuthorship W1998204844A5030221375 @default.
- W1998204844 hasAuthorship W1998204844A5063587224 @default.
- W1998204844 hasAuthorship W1998204844A5071760145 @default.
- W1998204844 hasBestOaLocation W19982048441 @default.
- W1998204844 hasConcept C12554922 @default.
- W1998204844 hasConcept C147944092 @default.
- W1998204844 hasConcept C152624196 @default.
- W1998204844 hasConcept C170493617 @default.
- W1998204844 hasConcept C178790620 @default.
- W1998204844 hasConcept C185592680 @default.
- W1998204844 hasConcept C18699975 @default.
- W1998204844 hasConcept C190062978 @default.
- W1998204844 hasConcept C2777427919 @default.
- W1998204844 hasConcept C50254741 @default.
- W1998204844 hasConcept C519063684 @default.
- W1998204844 hasConcept C55493867 @default.
- W1998204844 hasConcept C79879829 @default.
- W1998204844 hasConcept C86803240 @default.
- W1998204844 hasConceptScore W1998204844C12554922 @default.
- W1998204844 hasConceptScore W1998204844C147944092 @default.
- W1998204844 hasConceptScore W1998204844C152624196 @default.
- W1998204844 hasConceptScore W1998204844C170493617 @default.
- W1998204844 hasConceptScore W1998204844C178790620 @default.
- W1998204844 hasConceptScore W1998204844C185592680 @default.
- W1998204844 hasConceptScore W1998204844C18699975 @default.
- W1998204844 hasConceptScore W1998204844C190062978 @default.
- W1998204844 hasConceptScore W1998204844C2777427919 @default.
- W1998204844 hasConceptScore W1998204844C50254741 @default.
- W1998204844 hasConceptScore W1998204844C519063684 @default.
- W1998204844 hasConceptScore W1998204844C55493867 @default.
- W1998204844 hasConceptScore W1998204844C79879829 @default.
- W1998204844 hasConceptScore W1998204844C86803240 @default.
- W1998204844 hasIssue "3" @default.
- W1998204844 hasLocation W19982048441 @default.
- W1998204844 hasOpenAccess W1998204844 @default.
- W1998204844 hasPrimaryLocation W19982048441 @default.
- W1998204844 hasRelatedWork W1926574943 @default.
- W1998204844 hasRelatedWork W1998204844 @default.
- W1998204844 hasRelatedWork W2037727765 @default.
- W1998204844 hasRelatedWork W2042019088 @default.
- W1998204844 hasRelatedWork W2044742128 @default.
- W1998204844 hasRelatedWork W2058348515 @default.
- W1998204844 hasRelatedWork W2105810619 @default.
- W1998204844 hasRelatedWork W2374704004 @default.
- W1998204844 hasRelatedWork W2411861180 @default.
- W1998204844 hasRelatedWork W2049490721 @default.
- W1998204844 hasVolume "100" @default.
- W1998204844 isParatext "false" @default.
- W1998204844 isRetracted "false" @default.
- W1998204844 magId "1998204844" @default.
- W1998204844 workType "article" @default.