FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1998213056> ?p ?o ?g. }

• W1998213056 endingPage "S70" @default.
• W1998213056 startingPage "S69" @default.
• W1998213056 abstract "The Sleeping Beauty (SB) transposon is a Tc1/mariner family transposon that is being examined for its efficacy as a non-viral, integrating vector for human gene therapy. Integration of SB transposons, into TA dinucleotide base pairs, with respect to genes (exons and introns) and intergenic regions appears fairly random at the genomic level, but not on a micro-scale, with a previously reported consensus sequence around the TA-integration sites [Vigdal et al. (2002) J. Mol. Biol. 323: 441|[ndash]|452; Carlson et al. (2003) Genetics 165: 243|[ndash]|256]. In an earlier study [Liu et al. (2005) J. Mol. Biol. 346: 161|[ndash]|173], we analyzed target-site preferences of SB transposons using an intra-plasmid transposition assay. We found that preferential sites had a unique deformation pattern that was characterized by an off-center angling, non-uniform twisting of the DNA helix and an increase in the distance between the central base pairs at preferred target sites. Particular deformations of the double helix could be predicted by the Vstep algorithm, which distinguished three categories of TA sites that varied by about 16-fold in their acceptance of SB transposons: preferred, intermediate-preferred, and non-preferred. Here we report our results of integrations into TA sites in the ninth intron of the mouse B-raf gene. Transpositions occurred as a result of remobilization, by SB10 transposase, of chromosomally-resident |[ldquo]|insertionally oncogenic|[rdquo]| transposons in somatic tissues of transgenic mice. This resulted in oncogenic selection of 25 gain-of-function insertions into B-raf in p19Arf|[minus]|/|[minus]| mice [Collier and Carlson et al., submitted]. There are 349 potential TA-integration sites in the 4069-bp intron, of which 22 were targets for 25 unique insertions. The Vstep algorithm predicted preferential patterns for 44 of the 349 possible sites, of which 7 were hit and three were hit twice (a 23% hit rate). There were 107 predicted intermediate-preferred sites of which 11 were targets (10% hit rate), and 198 non-preferred sites of which 4 were hit (a 2% hit rate). These data strongly suggest about an 11-fold preference for integration at certain TA dinucleotide base pairs in chromatin, which can be predicted by the Vstep algorithm. This data suggests that a V-step algorithm can predict preferential insertion sites for transposon vectors during non-viral, SB-mediated gene transfer protocols." @default.
• W1998213056 created "2016-06-24" @default.
• W1998213056 creator A5005626540 @default.
• W1998213056 creator A5019240954 @default.
• W1998213056 creator A5031428652 @default.
• W1998213056 creator A5032924224 @default.
• W1998213056 creator A5037267842 @default.
• W1998213056 creator A5063827272 @default.
• W1998213056 creator A5070179606 @default.
• W1998213056 creator A5080734416 @default.
• W1998213056 date "2005-05-01" @default.
• W1998213056 modified "2023-10-18" @default.
• W1998213056 title "176. Target-Site Preferences of Sleeping Beauty Transposons in an Intron of the B-raf Oncogene" @default.
• W1998213056 doi "https://doi.org/10.1016/j.ymthe.2005.06.179" @default.
• W1998213056 hasPublicationYear "2005" @default.
• W1998213056 type Work @default.
• W1998213056 sameAs 1998213056 @default.
• W1998213056 citedByCount "0" @default.
• W1998213056 crossrefType "journal-article" @default.
• W1998213056 hasAuthorship W1998213056A5005626540 @default.
• W1998213056 hasAuthorship W1998213056A5019240954 @default.
• W1998213056 hasAuthorship W1998213056A5031428652 @default.
• W1998213056 hasAuthorship W1998213056A5032924224 @default.
• W1998213056 hasAuthorship W1998213056A5037267842 @default.
• W1998213056 hasAuthorship W1998213056A5063827272 @default.
• W1998213056 hasAuthorship W1998213056A5070179606 @default.
• W1998213056 hasAuthorship W1998213056A5080734416 @default.
• W1998213056 hasBestOaLocation W19982130561 @default.
• W1998213056 hasConcept C104317684 @default.
• W1998213056 hasConcept C107038049 @default.
• W1998213056 hasConcept C141231307 @default.
• W1998213056 hasConcept C142362112 @default.
• W1998213056 hasConcept C2780620123 @default.
• W1998213056 hasConcept C4918238 @default.
• W1998213056 hasConcept C54355233 @default.
• W1998213056 hasConcept C86803240 @default.
• W1998213056 hasConcept C94671646 @default.
• W1998213056 hasConceptScore W1998213056C104317684 @default.
• W1998213056 hasConceptScore W1998213056C107038049 @default.
• W1998213056 hasConceptScore W1998213056C141231307 @default.
• W1998213056 hasConceptScore W1998213056C142362112 @default.
• W1998213056 hasConceptScore W1998213056C2780620123 @default.
• W1998213056 hasConceptScore W1998213056C4918238 @default.
• W1998213056 hasConceptScore W1998213056C54355233 @default.
• W1998213056 hasConceptScore W1998213056C86803240 @default.
• W1998213056 hasConceptScore W1998213056C94671646 @default.
• W1998213056 hasLocation W19982130561 @default.
• W1998213056 hasOpenAccess W1998213056 @default.
• W1998213056 hasPrimaryLocation W19982130561 @default.
• W1998213056 hasRelatedWork W1986312970 @default.
• W1998213056 hasRelatedWork W1995112380 @default.
• W1998213056 hasRelatedWork W2014382116 @default.
• W1998213056 hasRelatedWork W2031623133 @default.
• W1998213056 hasRelatedWork W2035872214 @default.
• W1998213056 hasRelatedWork W2064609779 @default.
• W1998213056 hasRelatedWork W2102800408 @default.
• W1998213056 hasRelatedWork W2117502535 @default.
• W1998213056 hasRelatedWork W2129342151 @default.
• W1998213056 hasRelatedWork W2208028625 @default.
• W1998213056 hasVolume "11" @default.
• W1998213056 isParatext "false" @default.
• W1998213056 isRetracted "false" @default.
• W1998213056 magId "1998213056" @default.
• W1998213056 workType "article" @default.