Matches in SemOpenAlex for { <https://semopenalex.org/work/W1998252034> ?p ?o ?g. }
- W1998252034 endingPage "371" @default.
- W1998252034 startingPage "363" @default.
- W1998252034 abstract "The polymerase chain reaction (PCR) fails to detect HIV-1 sequences in 5% of infected individuals.1-3 To screen for false-negative PCR tests, we developed a nonisotopic PCR assay in which sequences from the β-globin gene and from the HIV-1 vpu-env region were coamplified with biotinylated and fluorescein-labeled primers, respectively. Coamplified products were reacted with specific internal digoxigenin-labeled RNA probes. Hybrids were detected in a microtiter plate coated with streptavidin or anti-fluorescein antibody, with enzyme-labeled anti-digoxigenin antibody. After the optimization of the coamplification conditions, the assay could detect 5 proviral DNA copies in a lysate from 100,000 peripheral blood mononuclear cells. Fifty-seven samples from 55 HIV-1-seropositive patients and 25 samples from 25 seronegative individuals were evaluated. Fifty-two samples from HIV-infected individuals were positive for HIV-1 vpu-env sequences. Three of the 5 PBMC lysates falsely negative for HIV-1 sequences had reactivities for β-globin (3-23 fu) below that of 100,000 cells (304 fu). Nonisotopic coamplification allowed for the evaluation of the quality of specimens for PCR concurrently with the detection of the presence of viral template sequences." @default.
- W1998252034 created "2016-06-24" @default.
- W1998252034 creator A5007711260 @default.
- W1998252034 creator A5015709853 @default.
- W1998252034 creator A5027614467 @default.
- W1998252034 creator A5047323770 @default.
- W1998252034 creator A5079949855 @default.
- W1998252034 date "1995-03-01" @default.
- W1998252034 modified "2023-09-27" @default.
- W1998252034 title "Coamplification of HIV Type 1 and β-Globin Gene DNA Sequences in a Nonisotopic Polymerase Chain Reaction Assay to Control for Amplification Efficiency" @default.
- W1998252034 cites W1536166248 @default.
- W1998252034 cites W1550018201 @default.
- W1998252034 cites W1574056158 @default.
- W1998252034 cites W1574646440 @default.
- W1998252034 cites W1579391803 @default.
- W1998252034 cites W1623490740 @default.
- W1998252034 cites W1871201083 @default.
- W1998252034 cites W1974590216 @default.
- W1998252034 cites W1977923433 @default.
- W1998252034 cites W1981228431 @default.
- W1998252034 cites W1987580201 @default.
- W1998252034 cites W1990273009 @default.
- W1998252034 cites W1999097855 @default.
- W1998252034 cites W2011893020 @default.
- W1998252034 cites W2014846266 @default.
- W1998252034 cites W2016537723 @default.
- W1998252034 cites W2019770663 @default.
- W1998252034 cites W2026242634 @default.
- W1998252034 cites W2026812392 @default.
- W1998252034 cites W2033249788 @default.
- W1998252034 cites W2046965609 @default.
- W1998252034 cites W2047480444 @default.
- W1998252034 cites W2054333657 @default.
- W1998252034 cites W2056224227 @default.
- W1998252034 cites W2071101555 @default.
- W1998252034 cites W2081010694 @default.
- W1998252034 cites W2084397293 @default.
- W1998252034 cites W2095339034 @default.
- W1998252034 cites W2095438774 @default.
- W1998252034 cites W2100442546 @default.
- W1998252034 cites W2101588264 @default.
- W1998252034 cites W2114738695 @default.
- W1998252034 cites W2128175070 @default.
- W1998252034 cites W2147243442 @default.
- W1998252034 cites W2149897099 @default.
- W1998252034 cites W2242391508 @default.
- W1998252034 cites W2331106135 @default.
- W1998252034 cites W4230066759 @default.
- W1998252034 cites W4241273753 @default.
- W1998252034 doi "https://doi.org/10.1089/aid.1995.11.363" @default.
- W1998252034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7786582" @default.
- W1998252034 hasPublicationYear "1995" @default.
- W1998252034 type Work @default.
- W1998252034 sameAs 1998252034 @default.
- W1998252034 citedByCount "20" @default.
- W1998252034 countsByYear W19982520342013 @default.
- W1998252034 countsByYear W19982520342014 @default.
- W1998252034 countsByYear W19982520342022 @default.
- W1998252034 crossrefType "journal-article" @default.
- W1998252034 hasAuthorship W1998252034A5007711260 @default.
- W1998252034 hasAuthorship W1998252034A5015709853 @default.
- W1998252034 hasAuthorship W1998252034A5027614467 @default.
- W1998252034 hasAuthorship W1998252034A5047323770 @default.
- W1998252034 hasAuthorship W1998252034A5079949855 @default.
- W1998252034 hasConcept C104317684 @default.
- W1998252034 hasConcept C105580179 @default.
- W1998252034 hasConcept C129312508 @default.
- W1998252034 hasConcept C153911025 @default.
- W1998252034 hasConcept C159047783 @default.
- W1998252034 hasConcept C204628709 @default.
- W1998252034 hasConcept C2776552150 @default.
- W1998252034 hasConcept C2779186596 @default.
- W1998252034 hasConcept C2781440490 @default.
- W1998252034 hasConcept C49105822 @default.
- W1998252034 hasConcept C552990157 @default.
- W1998252034 hasConcept C55493867 @default.
- W1998252034 hasConcept C81439078 @default.
- W1998252034 hasConcept C86803240 @default.
- W1998252034 hasConceptScore W1998252034C104317684 @default.
- W1998252034 hasConceptScore W1998252034C105580179 @default.
- W1998252034 hasConceptScore W1998252034C129312508 @default.
- W1998252034 hasConceptScore W1998252034C153911025 @default.
- W1998252034 hasConceptScore W1998252034C159047783 @default.
- W1998252034 hasConceptScore W1998252034C204628709 @default.
- W1998252034 hasConceptScore W1998252034C2776552150 @default.
- W1998252034 hasConceptScore W1998252034C2779186596 @default.
- W1998252034 hasConceptScore W1998252034C2781440490 @default.
- W1998252034 hasConceptScore W1998252034C49105822 @default.
- W1998252034 hasConceptScore W1998252034C552990157 @default.
- W1998252034 hasConceptScore W1998252034C55493867 @default.
- W1998252034 hasConceptScore W1998252034C81439078 @default.
- W1998252034 hasConceptScore W1998252034C86803240 @default.
- W1998252034 hasIssue "3" @default.
- W1998252034 hasLocation W19982520341 @default.
- W1998252034 hasLocation W19982520342 @default.
- W1998252034 hasOpenAccess W1998252034 @default.
- W1998252034 hasPrimaryLocation W19982520341 @default.
- W1998252034 hasRelatedWork W1574646440 @default.