FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1998286757> ?p ?o ?g. }

• W1998286757 endingPage "58" @default.
• W1998286757 startingPage "49" @default.
• W1998286757 abstract "By determination of the nucleotide sequence adjacent to the right border of T-DNA of the mikimopine-type Ri plasmid (pRi1724) in Agrobacterium rhizogenes, a new open reading frame (ORF) encoding 318 amino acids was found. A transcript of 1.35 kb derived from this ORF was observed in hairy roots of Ajuga reptans by northern blotting analysis. Including its own promoter and terminator, this ORF was isolated from the pRi1724 T-DNA and introduced into tobacco plants by the Agrobacterium-binary vector system. Since mikimopine, an opine and a stereoisomer of cucumopine, was accumulated in all organs of the transgenic tobacco plants, the new ORF was deduced to be the mikimopine synthase gene. For comparison, the nucleotide sequence of cucumopine synthase encoded on pRi2659 was also determined. No homology was found between mikimopine synthase and cucumopine synthase at the nucleotide, but partial homology was found at the amino acid level. Mikimopine synthase and cucumopine synthase produced by a protein expression system using E. coli catalyzed the synthesis of mikimopine and cucumopine from l-histidine and α-ketoglutaric acid, requiring NADH as a cofactor. These synthesized opines were identified by paper electrophoresis, TLC and HPLC analyses. The synthesized mikimopine or cucumopine could be degraded by A. rhizogenes strains harboring Ri plasmids encoding the respective catabolic enzyme." @default.
• W1998286757 created "2016-06-24" @default.
• W1998286757 creator A5007729309 @default.
• W1998286757 creator A5041996787 @default.
• W1998286757 creator A5048656899 @default.
• W1998286757 creator A5082370715 @default.
• W1998286757 date "2001-01-01" @default.
• W1998286757 modified "2023-09-24" @default.
• W1998286757 title "Mikimopine synthase ( mis ) gene on pRi1724" @default.
• W1998286757 cites W1549875078 @default.
• W1998286757 cites W1573199302 @default.
• W1998286757 cites W1976112025 @default.
• W1998286757 cites W1979602933 @default.
• W1998286757 cites W1985862581 @default.
• W1998286757 cites W2007668792 @default.
• W1998286757 cites W2024205709 @default.
• W1998286757 cites W2024556021 @default.
• W1998286757 cites W2037369328 @default.
• W1998286757 cites W2040809285 @default.
• W1998286757 cites W2043727303 @default.
• W1998286757 cites W2050694159 @default.
• W1998286757 cites W2065911300 @default.
• W1998286757 cites W2073316045 @default.
• W1998286757 cites W2078669789 @default.
• W1998286757 cites W2079429682 @default.
• W1998286757 cites W2082787436 @default.
• W1998286757 cites W2084980098 @default.
• W1998286757 cites W2095258434 @default.
• W1998286757 cites W2121314593 @default.
• W1998286757 cites W2130095521 @default.
• W1998286757 cites W2312801164 @default.
• W1998286757 cites W2313924593 @default.
• W1998286757 cites W2323771821 @default.
• W1998286757 cites W2329200441 @default.
• W1998286757 cites W2333056126 @default.
• W1998286757 cites W2334221246 @default.
• W1998286757 cites W316737317 @default.
• W1998286757 doi "https://doi.org/10.1016/s0378-1119(00)00578-3" @default.
• W1998286757 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11223242" @default.
• W1998286757 hasPublicationYear "2001" @default.
• W1998286757 type Work @default.
• W1998286757 sameAs 1998286757 @default.
• W1998286757 citedByCount "22" @default.
• W1998286757 countsByYear W19982867572012 @default.
• W1998286757 countsByYear W19982867572013 @default.
• W1998286757 countsByYear W19982867572015 @default.
• W1998286757 countsByYear W19982867572016 @default.
• W1998286757 countsByYear W19982867572018 @default.
• W1998286757 countsByYear W19982867572020 @default.
• W1998286757 countsByYear W19982867572021 @default.
• W1998286757 countsByYear W19982867572023 @default.
• W1998286757 crossrefType "journal-article" @default.
• W1998286757 hasAuthorship W1998286757A5007729309 @default.
• W1998286757 hasAuthorship W1998286757A5041996787 @default.
• W1998286757 hasAuthorship W1998286757A5048656899 @default.
• W1998286757 hasAuthorship W1998286757A5082370715 @default.
• W1998286757 hasConcept C104317684 @default.
• W1998286757 hasConcept C112243037 @default.
• W1998286757 hasConcept C116403925 @default.
• W1998286757 hasConcept C121332964 @default.
• W1998286757 hasConcept C1276947 @default.
• W1998286757 hasConcept C133307689 @default.
• W1998286757 hasConcept C153911025 @default.
• W1998286757 hasConcept C165525559 @default.
• W1998286757 hasConcept C167625842 @default.
• W1998286757 hasConcept C178046730 @default.
• W1998286757 hasConcept C204241405 @default.
• W1998286757 hasConcept C22744801 @default.
• W1998286757 hasConcept C2776162087 @default.
• W1998286757 hasConcept C2779656422 @default.
• W1998286757 hasConcept C33161422 @default.
• W1998286757 hasConcept C40767141 @default.
• W1998286757 hasConcept C47289529 @default.
• W1998286757 hasConcept C55493867 @default.
• W1998286757 hasConcept C84148353 @default.
• W1998286757 hasConcept C86803240 @default.
• W1998286757 hasConcept C98151556 @default.
• W1998286757 hasConceptScore W1998286757C104317684 @default.
• W1998286757 hasConceptScore W1998286757C112243037 @default.
• W1998286757 hasConceptScore W1998286757C116403925 @default.
• W1998286757 hasConceptScore W1998286757C121332964 @default.
• W1998286757 hasConceptScore W1998286757C1276947 @default.
• W1998286757 hasConceptScore W1998286757C133307689 @default.
• W1998286757 hasConceptScore W1998286757C153911025 @default.
• W1998286757 hasConceptScore W1998286757C165525559 @default.
• W1998286757 hasConceptScore W1998286757C167625842 @default.
• W1998286757 hasConceptScore W1998286757C178046730 @default.
• W1998286757 hasConceptScore W1998286757C204241405 @default.
• W1998286757 hasConceptScore W1998286757C22744801 @default.
• W1998286757 hasConceptScore W1998286757C2776162087 @default.
• W1998286757 hasConceptScore W1998286757C2779656422 @default.
• W1998286757 hasConceptScore W1998286757C33161422 @default.
• W1998286757 hasConceptScore W1998286757C40767141 @default.
• W1998286757 hasConceptScore W1998286757C47289529 @default.
• W1998286757 hasConceptScore W1998286757C55493867 @default.
• W1998286757 hasConceptScore W1998286757C84148353 @default.
• W1998286757 hasConceptScore W1998286757C86803240 @default.
• W1998286757 hasConceptScore W1998286757C98151556 @default.

• next