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- W1998317015 abstract "<h3>Objectives</h3> Senescence is involved in many age-related diseases, such as atherosclerosis, hypertension and diabetes. So how to delay or solve the problems caused by ageing has become a hot topic in the past decade. To explore the best stress-induced premature senescence (SIPS) model <i>in vitro</i>, we applied different dosages of H<sub>2</sub>O<sub>2 </sub>to the primary human umbilical vein endothelial cells (HUVECs). <h3>Methods</h3> SIPS was induced by H<sub>2</sub>O<sub>2 </sub>with different concentration (0, 20, 40, 60, 80, 100 μM) in HUVECs<sub>. </sub>Senescence related gene was evaluated with western blot or real-time PCR. Senescence-associated beta-galactosidase (SA-β-gal) staining was used to examine the phenotype of senescence. Apoptosis was analysed by flow cytometry. <h3>Results</h3> The SA-β-gal staining result showed that the senescent cell was detected more significantly with H<sub>2</sub>O<sub>2</sub>≥40 μM compared with the control group. Also the mRNA of p21 was upregulated significantly with 40μM H<sub>2</sub>O<sub>2</sub> and more. The PAI-1 protein was increased significantly with 60 μM H<sub>2</sub>O<sub>2 </sub>and more. In the apoptosis aspect, there was a significant increased number of apoptotic cells in 80 and 100 μM group. <h3>Conclusions</h3> H<sub>2</sub>O<sub>2</sub> can induce senescence of the primary HUVECs. In consideration of the its apoptotic effect, the 60 μM H<sub>2</sub>O<sub>2 </sub>could be the best concentration to induce senescence. Because of the high relevance with human, the primary HUVECs is a better candidate applied to explore the mechanism and the improved treatment of senescence <i>in vitro</i>. Besides H<sub>2</sub>O<sub>2</sub>, there are some other ways to induce senescence, such as UV and gene knock down. Among these methods, H<sub>2</sub>O<sub>2</sub> induced senescence via oxidative stress is the most relavent to the enviroment in the aged population." @default.
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- W1998317015 date "2013-08-01" @default.
- W1998317015 modified "2023-10-14" @default.
- W1998317015 title "GW24-e0410 Stress-induced premature senescence in primary human umbilical vein endothelial cells" @default.
- W1998317015 doi "https://doi.org/10.1136/heartjnl-2013-304613.128" @default.
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