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- W1998331569 abstract "This paper describes a plasmid transformation system that permits genetic manipulation of the genus Sphingomonas. A cryptic indigenous plasmid, pAMI-1, of 10 kb from Sphingobium amiense was isolated and characterized. Nucleotide sequencing revealed that pAMI-1 contains five open reading frames, which are predicted to encode proteins associated with integration, recombination, conjugation and replication. Escherichia coli-S. amiense shuttle vectors were successfully introduced into Sphingomonas, Sphingobium, Novosphingobium and Sphingopyxis strains by electroporation. The copy number of the shuttle vector was estimated to be 1-2 per chromosome in the Sphingobium yanoikuyae cell." @default.
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- W1998331569 date "2006-01-01" @default.
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- W1998331569 title "Genetic Transformation System for Members of the Genera, Sphingomonas, Sphingobium, Novosphingobium and Sphingopyxis" @default.
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- W1998331569 doi "https://doi.org/10.1264/jsme2.21.235" @default.
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