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• W1998345079 abstract "A study of the degradation of plant cell walls by the mixture of enzymes present in Pectinol R-10 is described. A wall-modifying enzyme has been purified from this mixture by a combination of diethylaminoethyl cellulose, Bio Gel P-100, and carboxymethyl cellulose chromatography. Treatment of cell walls with the wall-modifying enzyme is shown to be a necessary prerequisite to wall degradation catalyzed by a mixture of polysaccharide-degrading enzymes prepared from Pectinol R-10 or by an alpha-galactosidase secreted by the pathogenic fungus Colletotrichum lindemuthianum. The action of the wall-modifying enzyme on cell walls is shown to result in both a release of water-soluble, 70% ethanol-insoluble polymers and an alteration of the residual cell wall. A purified preparation of the wall-modifying enzyme is unable to degrade a wide variety of polysaccharide, glycoside, and peptide substrates. However, the purified preparation of wall-modifying enzyme has a limited ability to degrade polygalacturonic acid. The fact that polygalacturonic acid inhibits the ability of the wall-modifying enzyme to affect cell walls suggests that the wall-modifying enzyme may be responsible for the limited polygalacturonic acid-degrading activity present in the purified preparation. The importance of a wall-modifying enzyme in developmental processes and in pathogenesis is discussed." @default.
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• W1998345079 date "1970-07-01" @default.
• W1998345079 modified "2023-09-27" @default.
• W1998345079 title "Polysaccharide-degrading Enzymes are Unable to Attack Plant Cell Walls without Prior Action by a “Wall-modifying Enzyme”" @default.
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• W1998345079 doi "https://doi.org/10.1104/pp.46.1.69" @default.
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