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- W1998415432 abstract "Monocyte/macrophage adhesion to biomaterials, correlated with foreign body response, occurs through protein-mediated surface interactions. Albumin-selective perfluorocarbon (FC) biomaterials are generally poorly cell-conducive because of insufficient receptor-mediated surface interactions, but macrophages bind to albumin-coated substrates and also preferentially to highly hydrophobic fluorinated surfaces. Bone marrow macrophages (BMMO) and IC-21, RAW 264.7, and J774A.1 monocyte/macrophage cells were cultured on FC surfaces. Protein deposition onto two distinct FC surfaces from complex and single-component solutions was tracked using fluorescence and time-of-flight secondary ion mass spectrometry (ToF-SIMS) methods. Cell adhesion and growth on protein pretreated substrates were compared by light microscopy. Flow cytometry and integrin-directed antibody receptor blocking were used to assess integrins critical for monocyte/macrophage adhesion in vitro. Albumin predominantly adsorbs onto both FC surfaces from 10% serum. In cultures preadsorbed with albumin or serum-dilutions, BMMO responded similar to IC-21 at early time points. Compared with Teflon AF, plasma-polymerized FC was less permissive to extended cell proliferation. The beta(2) integrins play major roles in macrophage adhesion to FC surfaces: antibody blocking significantly disrupted cell adhesion. Albumin-mediated cell adhesion mechanisms to FC surfaces could not be clarified. Primary BMMO and secondary IC-21 macrophages behave similarly on FC surfaces, regardless of preadsorbed protein biasing, with respect to adhesion, cell morphology, motility, and proliferation." @default.
- W1998415432 created "2016-06-24" @default.
- W1998415432 creator A5009060646 @default.
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- W1998415432 creator A5034127767 @default.
- W1998415432 creator A5040402422 @default.
- W1998415432 creator A5085157952 @default.
- W1998415432 date "2008-02-27" @default.
- W1998415432 modified "2023-10-12" @default.
- W1998415432 title "Adsorbed serum albumin is permissive to macrophage attachment to perfluorocarbon polymer surfaces in culture" @default.
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- W1998415432 doi "https://doi.org/10.1002/jbm.a.31886" @default.
- W1998415432 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3801198" @default.
- W1998415432 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18306309" @default.
- W1998415432 hasPublicationYear "2008" @default.
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