FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1998450146> ?p ?o ?g. }

• W1998450146 endingPage "199" @default.
• W1998450146 startingPage "187" @default.
• W1998450146 abstract "Small conductance calcium (Ca2+) activated SK channels are critical regulators of neuronal excitability in hippocampus. Accordingly, these channels are thought to play a key role in controlling neuronal activity in acute models of epilepsy. In this study, we investigate the expression and function of SK channels in the pilocarpine model of mesial temporal lobe epilepsy. For this purpose, protein expression was assessed using western blotting assays and gene expression was analyzed using TaqMan-based probes and the quantitative real-time polymerase chain reaction (qPCR) comparative method delta-delta cycle threshold (△△CT) in samples extracted from control and epileptic rats. In addition, the effect of SK channel antagonist UCL1684 and agonist NS309 on CA1 evoked population spikes was studied in hippocampal slices. Western blotting analysis showed a significant reduction in the expression of SK1 and SK2 channels at 10 days following status epilepticus (SE), but levels recovered at 1 month and at more than 2 months after SE. In contrast, a significant down-regulation of SK3 channels was detected after 10 days of SE. Analysis of gene expression by qPCR revealed a significant reduction of transcripts for SK2 (Kcnn1) and SK3 (Kcnn3) channels as early as 10 days following pilocarpine-induced SE and during the chronic phase of the pilocarpine model. Moreover, bath application of UCL1684 (100 nM for 15 min) induced a significant increase of the population spike amplitude and number of spikes in the hippocampal CA1 area of slices obtained control and chronic epileptic rats. This effect was obliterated by co-administration of UCL1684 with SK channel agonist NS309 (1 μM). Application of NS309 failed to modify population spikes in the CA1 area of slices taken from control and epileptic rats. These data indicate an abnormal expression of SK channels and a possible dysfunction of these channels in experimental MTLE." @default.
• W1998450146 created "2016-06-24" @default.
• W1998450146 creator A5015281204 @default.
• W1998450146 creator A5023872830 @default.
• W1998450146 creator A5027108951 @default.
• W1998450146 creator A5030522185 @default.
• W1998450146 creator A5052413743 @default.
• W1998450146 creator A5058347824 @default.
• W1998450146 creator A5063480942 @default.
• W1998450146 creator A5064518099 @default.
• W1998450146 creator A5069257888 @default.
• W1998450146 date "2010-08-01" @default.
• W1998450146 modified "2023-10-17" @default.
• W1998450146 title "Altered expression and function of small-conductance (SK) Ca2+-activated K+ channels in pilocarpine-treated epileptic rats" @default.
• W1998450146 cites W1490915066 @default.
• W1998450146 cites W1537329448 @default.
• W1998450146 cites W1963624422 @default.
• W1998450146 cites W1963961001 @default.
• W1998450146 cites W1964326082 @default.
• W1998450146 cites W1964991820 @default.
• W1998450146 cites W1965162255 @default.
• W1998450146 cites W1971977652 @default.
• W1998450146 cites W1976021701 @default.
• W1998450146 cites W1977640234 @default.
• W1998450146 cites W1979530856 @default.
• W1998450146 cites W1980635947 @default.
• W1998450146 cites W1985958365 @default.
• W1998450146 cites W1988209218 @default.
• W1998450146 cites W1990546211 @default.
• W1998450146 cites W1993938369 @default.
• W1998450146 cites W1995577211 @default.
• W1998450146 cites W2000072607 @default.
• W1998450146 cites W2012776929 @default.
• W1998450146 cites W2013055611 @default.
• W1998450146 cites W2015378456 @default.
• W1998450146 cites W2017083014 @default.
• W1998450146 cites W2020477012 @default.
• W1998450146 cites W2023465178 @default.
• W1998450146 cites W2024590204 @default.
• W1998450146 cites W2026183536 @default.
• W1998450146 cites W2029554027 @default.
• W1998450146 cites W2031575360 @default.
• W1998450146 cites W2033849297 @default.
• W1998450146 cites W2033908484 @default.
• W1998450146 cites W2034271730 @default.
• W1998450146 cites W2036372135 @default.
• W1998450146 cites W2036686141 @default.
• W1998450146 cites W2041093905 @default.
• W1998450146 cites W2042670053 @default.
• W1998450146 cites W2043822158 @default.
• W1998450146 cites W2046656676 @default.
• W1998450146 cites W2047523290 @default.
• W1998450146 cites W2048528277 @default.
• W1998450146 cites W2049219701 @default.
• W1998450146 cites W2060258595 @default.
• W1998450146 cites W2065693649 @default.
• W1998450146 cites W2068083065 @default.
• W1998450146 cites W2069168092 @default.
• W1998450146 cites W2073049800 @default.
• W1998450146 cites W2076302671 @default.
• W1998450146 cites W2078156825 @default.
• W1998450146 cites W2085992606 @default.
• W1998450146 cites W2087519172 @default.
• W1998450146 cites W2091092254 @default.
• W1998450146 cites W2091417303 @default.
• W1998450146 cites W2099220026 @default.
• W1998450146 cites W2103946141 @default.
• W1998450146 cites W2109446991 @default.
• W1998450146 cites W2111070504 @default.
• W1998450146 cites W2114385744 @default.
• W1998450146 cites W2124201800 @default.
• W1998450146 cites W2125556242 @default.
• W1998450146 cites W2127296441 @default.
• W1998450146 cites W2127586922 @default.
• W1998450146 cites W2128224468 @default.
• W1998450146 cites W2131952724 @default.
• W1998450146 cites W2134187853 @default.
• W1998450146 cites W2145517203 @default.
• W1998450146 cites W2148428950 @default.
• W1998450146 cites W2155064011 @default.
• W1998450146 cites W2155889586 @default.
• W1998450146 cites W2157754546 @default.
• W1998450146 cites W2168849139 @default.
• W1998450146 cites W2170538656 @default.
• W1998450146 cites W2325334271 @default.
• W1998450146 cites W2416233525 @default.
• W1998450146 cites W2949872895 @default.
• W1998450146 cites W33280757 @default.
• W1998450146 doi "https://doi.org/10.1016/j.brainres.2010.05.095" @default.
• W1998450146 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2916930" @default.
• W1998450146 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20553876" @default.
• W1998450146 hasPublicationYear "2010" @default.
• W1998450146 type Work @default.
• W1998450146 sameAs 1998450146 @default.
• W1998450146 citedByCount "38" @default.
• W1998450146 countsByYear W19984501462012 @default.
• W1998450146 countsByYear W19984501462013 @default.
• W1998450146 countsByYear W19984501462014 @default.

• next