Matches in SemOpenAlex for { <https://semopenalex.org/work/W1998469586> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1998469586 endingPage "4835" @default.
- W1998469586 startingPage "4827" @default.
- W1998469586 abstract "The use of CE for rapid assessment of metabolic stability of drugs with cytochrome P450 (CYP) enzymes, based on relative rates of reduced nicotinamide adenine dinucleotide phosphate (NADPH) consumption and nicotinamide adenine dinucleotide phosphate (NADP) production, was investigated. The separation conditions were as follows: capillary, 80.5 cm (75 microm id, 72 cm effective length for UV detection, 58 cm effective length for fluorescence detection); 25 mM sodium phosphate buffer (pH 8.8); 28 kV (80 microA) applied voltage; UV, 260 nm; fluorescence detection, excitation wavelength, 310 nm, emission wavelength, 418 nm; capillary temperature, 25 degrees C. For UV detection, the incubation conditions were as follows: CYP3A4: 20 pmol/mL; NADPH: 1 mM; EDTA: 1 mM; concentration of the substrate: 5-10 times its reported literature K(m) value; temperature: 37 degrees C; incubation time: 15 min. For fluorescence detection, the concentrations were reduced to CYP3A4: 4 pmol/mL, NADPH: 20 microM, EDTA: 20 microM and substrate: 10 microM. Blank incubations were performed in the absence of substrate. Compared with the blank, significant differences were found for the consumption of NADPH and the production of NADP. The development of this assay system allows rapid assessment of metabolic stability relative to standard compounds, as well as potential identification of the major CYP involved in the metabolism. It would reduce the backlog of compounds that require LC/MS analysis, and thereby expedite the process of metabolic stability screening." @default.
- W1998469586 created "2016-06-24" @default.
- W1998469586 creator A5004798967 @default.
- W1998469586 creator A5006769756 @default.
- W1998469586 creator A5014597760 @default.
- W1998469586 creator A5040817120 @default.
- W1998469586 date "2006-11-29" @default.
- W1998469586 modified "2023-10-16" @default.
- W1998469586 title "Screening of drug metabolism by CE" @default.
- W1998469586 cites W1508691659 @default.
- W1998469586 cites W1578275753 @default.
- W1998469586 cites W1982354039 @default.
- W1998469586 cites W1996485240 @default.
- W1998469586 cites W2003017820 @default.
- W1998469586 cites W2019147690 @default.
- W1998469586 cites W2032488910 @default.
- W1998469586 cites W2048992861 @default.
- W1998469586 cites W2066099560 @default.
- W1998469586 cites W2070213952 @default.
- W1998469586 cites W2079057400 @default.
- W1998469586 cites W2092057762 @default.
- W1998469586 cites W2097100227 @default.
- W1998469586 cites W2130004816 @default.
- W1998469586 cites W4246785877 @default.
- W1998469586 cites W2028365997 @default.
- W1998469586 doi "https://doi.org/10.1002/elps.200600163" @default.
- W1998469586 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17080490" @default.
- W1998469586 hasPublicationYear "2006" @default.
- W1998469586 type Work @default.
- W1998469586 sameAs 1998469586 @default.
- W1998469586 citedByCount "6" @default.
- W1998469586 countsByYear W19984695862013 @default.
- W1998469586 crossrefType "journal-article" @default.
- W1998469586 hasAuthorship W1998469586A5004798967 @default.
- W1998469586 hasAuthorship W1998469586A5006769756 @default.
- W1998469586 hasAuthorship W1998469586A5014597760 @default.
- W1998469586 hasAuthorship W1998469586A5040817120 @default.
- W1998469586 hasConcept C121332964 @default.
- W1998469586 hasConcept C181199279 @default.
- W1998469586 hasConcept C185592680 @default.
- W1998469586 hasConcept C18903297 @default.
- W1998469586 hasConcept C2776317432 @default.
- W1998469586 hasConcept C2776901170 @default.
- W1998469586 hasConcept C2777289219 @default.
- W1998469586 hasConcept C2777989768 @default.
- W1998469586 hasConcept C2779728014 @default.
- W1998469586 hasConcept C38485361 @default.
- W1998469586 hasConcept C43617362 @default.
- W1998469586 hasConcept C55493867 @default.
- W1998469586 hasConcept C62231903 @default.
- W1998469586 hasConcept C62520636 @default.
- W1998469586 hasConcept C75520062 @default.
- W1998469586 hasConcept C86803240 @default.
- W1998469586 hasConcept C91881484 @default.
- W1998469586 hasConceptScore W1998469586C121332964 @default.
- W1998469586 hasConceptScore W1998469586C181199279 @default.
- W1998469586 hasConceptScore W1998469586C185592680 @default.
- W1998469586 hasConceptScore W1998469586C18903297 @default.
- W1998469586 hasConceptScore W1998469586C2776317432 @default.
- W1998469586 hasConceptScore W1998469586C2776901170 @default.
- W1998469586 hasConceptScore W1998469586C2777289219 @default.
- W1998469586 hasConceptScore W1998469586C2777989768 @default.
- W1998469586 hasConceptScore W1998469586C2779728014 @default.
- W1998469586 hasConceptScore W1998469586C38485361 @default.
- W1998469586 hasConceptScore W1998469586C43617362 @default.
- W1998469586 hasConceptScore W1998469586C55493867 @default.
- W1998469586 hasConceptScore W1998469586C62231903 @default.
- W1998469586 hasConceptScore W1998469586C62520636 @default.
- W1998469586 hasConceptScore W1998469586C75520062 @default.
- W1998469586 hasConceptScore W1998469586C86803240 @default.
- W1998469586 hasConceptScore W1998469586C91881484 @default.
- W1998469586 hasIssue "23" @default.
- W1998469586 hasLocation W19984695861 @default.
- W1998469586 hasLocation W19984695862 @default.
- W1998469586 hasOpenAccess W1998469586 @default.
- W1998469586 hasPrimaryLocation W19984695861 @default.
- W1998469586 hasRelatedWork W1515195988 @default.
- W1998469586 hasRelatedWork W1585891632 @default.
- W1998469586 hasRelatedWork W1656364975 @default.
- W1998469586 hasRelatedWork W1820848506 @default.
- W1998469586 hasRelatedWork W2010411628 @default.
- W1998469586 hasRelatedWork W2130880511 @default.
- W1998469586 hasRelatedWork W2137996948 @default.
- W1998469586 hasRelatedWork W2158094397 @default.
- W1998469586 hasRelatedWork W2171340535 @default.
- W1998469586 hasRelatedWork W3149614979 @default.
- W1998469586 hasVolume "27" @default.
- W1998469586 isParatext "false" @default.
- W1998469586 isRetracted "false" @default.
- W1998469586 magId "1998469586" @default.
- W1998469586 workType "article" @default.