FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1998472816> ?p ?o ?g. }

• W1998472816 endingPage "66" @default.
• W1998472816 startingPage "61" @default.
• W1998472816 abstract "The effects of six recombinant human cytokines: erythropoietin, GM-CSF, G-CSF, interleukin-3, -4 and -6 on the proliferation and differentiation of a human multilineage myeloid leukemia cell line MHH 225, established from the bone marrow of an AML(M7) patient in our laboratory determined by changes in antigen expressions using monoclonal antibodies in APAAP technique were examined in liquid suspension culture. The MHH 225 cells have been growing exponentially without cytokines or conditioned media. About 90 per cent of MHH 225 cells are CD33+CD34+CD3–CD7–CD19–CD20–TdT– with 57.6 per cent, 28.3 per cent and 7.8 per cent of them being CD41+, glycophorin A+ and CD15+, respectively. After five days of treatment with erythropoietin, GM-CSF, G-CSF or IL-6 no change was observed in MHH 225 cell antigens expression. IL-3 (100 U/ml) induced a moderate increase in only CD13 and alpha naphthyl esterase positive cells from 6.5 ± 1.9 per cent and 5.7 ± 2.4 per cent in control cultures to 21.6 ± 3.0 per cent and 19.1 ± 2.8 per cent, respectively. On the other hand, 100 U/ml IL-4 significantly increased the number of CD13, CD15 and alpha naphthyl esterase positive cells to 48.9 ± 5.0 per cent, 47.2 ± 3.6 per cent and 46.1 ± 3.0 per cent, p < 0.001, respectively. Also, 100 U/ml IL-4 decreased the number of CD41 positive cells from 57.6 ± 2.8 per cent to only 25.9 ± 3.6 per cent and did not change the number of CD33 or glycophorin A positive cells. The present results showed that out of the six myelopoietic growth factors tested, IL-4 was the only one to inhibit selectively the proliferation of CD33+CD41+ leukemic megakaryoblast cells suggesting that IL-4 may have a lineage regulatory effect in favour of a myeloblastic CD33+CD13+CD15+ at the expense of a megakaryoblastic CD33+CD41+ amplification in human leukemia cells and with apparently no effect on leukemic erythroblast cells. The MHH 225 cell line provides a useful tool and freely available model to scientists for studying signal transduction via IL-4 and for studies of‘lineage switch’." @default.
• W1998472816 created "2016-06-24" @default.
• W1998472816 creator A5005594026 @default.
• W1998472816 creator A5024022763 @default.
• W1998472816 creator A5030739249 @default.
• W1998472816 creator A5084765360 @default.
• W1998472816 date "1994-01-01" @default.
• W1998472816 modified "2023-09-26" @default.
• W1998472816 title "Interleukin-4 inhibits proliferation of human leukemic megakaryoblast cell line mhh 225" @default.
• W1998472816 cites W128474680 @default.
• W1998472816 cites W1990034700 @default.
• W1998472816 cites W2014618815 @default.
• W1998472816 cites W2039637009 @default.
• W1998472816 cites W2058983653 @default.
• W1998472816 cites W2063063062 @default.
• W1998472816 cites W2320197189 @default.
• W1998472816 cites W2415780251 @default.
• W1998472816 cites W2417680863 @default.
• W1998472816 doi "https://doi.org/10.1002/hon.2900120203" @default.
• W1998472816 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7520882" @default.
• W1998472816 hasPublicationYear "1994" @default.
• W1998472816 type Work @default.
• W1998472816 sameAs 1998472816 @default.
• W1998472816 citedByCount "8" @default.
• W1998472816 crossrefType "journal-article" @default.
• W1998472816 hasAuthorship W1998472816A5005594026 @default.
• W1998472816 hasAuthorship W1998472816A5024022763 @default.
• W1998472816 hasAuthorship W1998472816A5030739249 @default.
• W1998472816 hasAuthorship W1998472816A5084765360 @default.
• W1998472816 hasConcept C10205521 @default.
• W1998472816 hasConcept C126322002 @default.
• W1998472816 hasConcept C147483822 @default.
• W1998472816 hasConcept C153911025 @default.
• W1998472816 hasConcept C16685009 @default.
• W1998472816 hasConcept C176290653 @default.
• W1998472816 hasConcept C203014093 @default.
• W1998472816 hasConcept C2776804306 @default.
• W1998472816 hasConcept C2778957590 @default.
• W1998472816 hasConcept C28328180 @default.
• W1998472816 hasConcept C54355233 @default.
• W1998472816 hasConcept C71924100 @default.
• W1998472816 hasConcept C81885089 @default.
• W1998472816 hasConcept C86803240 @default.
• W1998472816 hasConceptScore W1998472816C10205521 @default.
• W1998472816 hasConceptScore W1998472816C126322002 @default.
• W1998472816 hasConceptScore W1998472816C147483822 @default.
• W1998472816 hasConceptScore W1998472816C153911025 @default.
• W1998472816 hasConceptScore W1998472816C16685009 @default.
• W1998472816 hasConceptScore W1998472816C176290653 @default.
• W1998472816 hasConceptScore W1998472816C203014093 @default.
• W1998472816 hasConceptScore W1998472816C2776804306 @default.
• W1998472816 hasConceptScore W1998472816C2778957590 @default.
• W1998472816 hasConceptScore W1998472816C28328180 @default.
• W1998472816 hasConceptScore W1998472816C54355233 @default.
• W1998472816 hasConceptScore W1998472816C71924100 @default.
• W1998472816 hasConceptScore W1998472816C81885089 @default.
• W1998472816 hasConceptScore W1998472816C86803240 @default.
• W1998472816 hasIssue "2" @default.
• W1998472816 hasLocation W19984728161 @default.
• W1998472816 hasLocation W19984728162 @default.
• W1998472816 hasOpenAccess W1998472816 @default.
• W1998472816 hasPrimaryLocation W19984728161 @default.
• W1998472816 hasRelatedWork W1982666420 @default.
• W1998472816 hasRelatedWork W2278528399 @default.
• W1998472816 hasRelatedWork W2349509386 @default.
• W1998472816 hasRelatedWork W2350703333 @default.
• W1998472816 hasRelatedWork W2367828477 @default.
• W1998472816 hasRelatedWork W2370052381 @default.
• W1998472816 hasRelatedWork W2372776264 @default.
• W1998472816 hasRelatedWork W2383687082 @default.
• W1998472816 hasRelatedWork W2385993229 @default.
• W1998472816 hasRelatedWork W2411086946 @default.
• W1998472816 hasVolume "12" @default.
• W1998472816 isParatext "false" @default.
• W1998472816 isRetracted "false" @default.
• W1998472816 magId "1998472816" @default.
• W1998472816 workType "article" @default.