Matches in SemOpenAlex for { <https://semopenalex.org/work/W1998596873> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W1998596873 endingPage "12154" @default.
- W1998596873 startingPage "12147" @default.
- W1998596873 abstract "Poly(ADP-ribose) polymerase (PARP) is a multifunctional nuclear zinc finger protein which participates in the immediate response of mammalian cells exposed to DNA damaging agents. Given the complexity of the poly(ADP-ribosylation) reaction, we developed a large-scale screening procedure in Escherichia coli to identify randomly amino acids involved in the various aspects of this mechanism. Random mutations were generated by the polymerase chain reaction in a cDNA sequence covering most of the catalytic domain. Out of 26 individual mutations that diversely inactivated the full-length PARP, 22 were found at conserved positions in the primary structure, and 24 were located in the core domain formed by two β-sheets containing the active site. Most of the PARP mutants were altered in poly(ADP-ribose) elongation and/or branching. The spatial proximity of some residues involved in chain elongation (E988) and branching (Y986) suggests a proximity or a superposition of these two catalytic sites. Other residues affected in branching were located at the surface of the molecule (R847, E923, G972), indicating that protein−protein contacts are necessary for optimal polymer branching. This screening procedure provides a simple and efficient method to explore further the structure−function relationship of the enzyme." @default.
- W1998596873 created "2016-06-24" @default.
- W1998596873 creator A5002462049 @default.
- W1998596873 creator A5006677369 @default.
- W1998596873 creator A5052159273 @default.
- W1998596873 creator A5057922948 @default.
- W1998596873 date "1997-10-01" @default.
- W1998596873 modified "2023-09-24" @default.
- W1998596873 title "Random Mutagenesis of the Poly(ADP-ribose) Polymerase Catalytic Domain Reveals Amino Acids Involved in Polymer Branching" @default.
- W1998596873 cites W123340411 @default.
- W1998596873 cites W1487601077 @default.
- W1998596873 cites W1505396363 @default.
- W1998596873 cites W1530211929 @default.
- W1998596873 cites W1552451187 @default.
- W1998596873 cites W1605052148 @default.
- W1998596873 cites W1903029482 @default.
- W1998596873 cites W1972954693 @default.
- W1998596873 cites W2055505026 @default.
- W1998596873 cites W2090685385 @default.
- W1998596873 cites W2096213915 @default.
- W1998596873 cites W2122139577 @default.
- W1998596873 cites W4246142592 @default.
- W1998596873 doi "https://doi.org/10.1021/bi971055p" @default.
- W1998596873 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9315851" @default.
- W1998596873 hasPublicationYear "1997" @default.
- W1998596873 type Work @default.
- W1998596873 sameAs 1998596873 @default.
- W1998596873 citedByCount "120" @default.
- W1998596873 countsByYear W19985968732012 @default.
- W1998596873 countsByYear W19985968732013 @default.
- W1998596873 countsByYear W19985968732014 @default.
- W1998596873 countsByYear W19985968732015 @default.
- W1998596873 countsByYear W19985968732016 @default.
- W1998596873 countsByYear W19985968732017 @default.
- W1998596873 countsByYear W19985968732018 @default.
- W1998596873 countsByYear W19985968732019 @default.
- W1998596873 countsByYear W19985968732020 @default.
- W1998596873 countsByYear W19985968732021 @default.
- W1998596873 countsByYear W19985968732022 @default.
- W1998596873 countsByYear W19985968732023 @default.
- W1998596873 crossrefType "journal-article" @default.
- W1998596873 hasAuthorship W1998596873A5002462049 @default.
- W1998596873 hasAuthorship W1998596873A5006677369 @default.
- W1998596873 hasAuthorship W1998596873A5052159273 @default.
- W1998596873 hasAuthorship W1998596873A5057922948 @default.
- W1998596873 hasConcept C104317684 @default.
- W1998596873 hasConcept C143065580 @default.
- W1998596873 hasConcept C16318435 @default.
- W1998596873 hasConcept C181199279 @default.
- W1998596873 hasConcept C182979987 @default.
- W1998596873 hasConcept C185592680 @default.
- W1998596873 hasConcept C45510557 @default.
- W1998596873 hasConcept C515207424 @default.
- W1998596873 hasConcept C552990157 @default.
- W1998596873 hasConcept C55493867 @default.
- W1998596873 hasConcept C75520062 @default.
- W1998596873 hasConcept C82381507 @default.
- W1998596873 hasConcept C86803240 @default.
- W1998596873 hasConceptScore W1998596873C104317684 @default.
- W1998596873 hasConceptScore W1998596873C143065580 @default.
- W1998596873 hasConceptScore W1998596873C16318435 @default.
- W1998596873 hasConceptScore W1998596873C181199279 @default.
- W1998596873 hasConceptScore W1998596873C182979987 @default.
- W1998596873 hasConceptScore W1998596873C185592680 @default.
- W1998596873 hasConceptScore W1998596873C45510557 @default.
- W1998596873 hasConceptScore W1998596873C515207424 @default.
- W1998596873 hasConceptScore W1998596873C552990157 @default.
- W1998596873 hasConceptScore W1998596873C55493867 @default.
- W1998596873 hasConceptScore W1998596873C75520062 @default.
- W1998596873 hasConceptScore W1998596873C82381507 @default.
- W1998596873 hasConceptScore W1998596873C86803240 @default.
- W1998596873 hasIssue "40" @default.
- W1998596873 hasLocation W19985968731 @default.
- W1998596873 hasLocation W19985968732 @default.
- W1998596873 hasLocation W19985968733 @default.
- W1998596873 hasLocation W19985968734 @default.
- W1998596873 hasOpenAccess W1998596873 @default.
- W1998596873 hasPrimaryLocation W19985968731 @default.
- W1998596873 hasRelatedWork W1541282095 @default.
- W1998596873 hasRelatedWork W1596209594 @default.
- W1998596873 hasRelatedWork W1644178486 @default.
- W1998596873 hasRelatedWork W184207458 @default.
- W1998596873 hasRelatedWork W1976892794 @default.
- W1998596873 hasRelatedWork W1991793578 @default.
- W1998596873 hasRelatedWork W2016517525 @default.
- W1998596873 hasRelatedWork W2461429950 @default.
- W1998596873 hasRelatedWork W258580054 @default.
- W1998596873 hasRelatedWork W2887912390 @default.
- W1998596873 hasVolume "36" @default.
- W1998596873 isParatext "false" @default.
- W1998596873 isRetracted "false" @default.
- W1998596873 magId "1998596873" @default.
- W1998596873 workType "article" @default.