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- W1998628072 abstract "Quantitative analysis of carbohydrates is a fundamental analytical tool used in many aspects of biology and chemistry. We have adapted a technique developed by Mathews et al. using an inexpensive scanner and open-source image analysis software to quantify amylase activity using both the breakdown of starch and the appearance of glucose. Breakdown of starch is analyzed using the iodide/iodine reagent, while the appearance of glucose is followed using Benedict's reagent. The progress of Benedict's reagent is quantified using the inherent color change of a blue solution into a red precipitate. The reaction velocity at different time points is then plotted and graphed using an Excel spreadsheet. This laboratory emphasizes that enzymes mediate the conversion of a substrate into a product and that either the concentration of product or reactant may be used to follow the course of a reaction." @default.
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- W1998628072 date "2008-03-01" @default.
- W1998628072 modified "2023-10-15" @default.
- W1998628072 title "Kinetic Analysis of Amylase Using Quantitative Benedict's and Iodine Starch Reagents" @default.
- W1998628072 cites W1494911365 @default.
- W1998628072 doi "https://doi.org/10.1021/ed085p401" @default.
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