FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1998640908> ?p ?o ?g. }

• W1998640908 endingPage "11000" @default.
• W1998640908 startingPage "10990" @default.
• W1998640908 abstract "The serine/threonine mammalian sterile 20-like kinase (MST1) is involved in promotion of caspase-dependent and independent apoptosis. Phosphorylation and oligomerization are required for its activation. The oligomerization domain, denoted as SARAH domain, forms an antiparallel coiled coil dimer, and it is important for both MST1 autophosphorylation and interactions with other proteins like the Rassf proteins containing also a SARAH domain. Here we show that the monomeric state of SARAH is thermodynamically unstable and that homodimerization is coupled with folding. Moreover, the influence of the inhibitory domain on SARAH stability and affinity is addressed. By investigating the thermal denaturation using differential scanning calorimetry and circular dichroism, we have found that the SARAH domain dissociates and unfolds cooperatively, without a stable intermediate monomeric state. Combining the data with information from isothermal titration calorimetry, a low thermodynamic stability of the monomeric species is obtained. Thus, it is proposed that the transition from MST1 SARAH homodimer to some specific heterodimer implies a non-native monomer intermediate. The inhibitory domain is found to be highly flexible and intrinsically unfolded, not only in isolation but also in the dimeric state of the inhibitory-SARAH construct. The existence of two caspase recognition motifs within the inhibitory domain suggests that its structural flexibility might be important for activation of MST1 during apoptosis. Moreover, the inhibitory domain increases the thermodynamic stability of the SARAH dimer and the homodimer affinity, while having almost no effect on the SARAH domain in the monomeric state. These results emphasize the importance of flexibility and binding-induced folding for specificity, affinity, and the capacity to switch from one state to another." @default.
• W1998640908 created "2016-06-24" @default.
• W1998640908 creator A5023135666 @default.
• W1998640908 creator A5036230479 @default.
• W1998640908 creator A5057675654 @default.
• W1998640908 creator A5072704915 @default.
• W1998640908 creator A5074643696 @default.
• W1998640908 date "2011-12-02" @default.
• W1998640908 modified "2023-09-26" @default.
• W1998640908 title "Dimerization-Induced Folding of MST1 SARAH and the Influence of the Intrinsically Unstructured Inhibitory Domain: Low Thermodynamic Stability of Monomer" @default.
• W1998640908 cites W1494988280 @default.
• W1998640908 cites W1591764649 @default.
• W1998640908 cites W1605533832 @default.
• W1998640908 cites W166851970 @default.
• W1998640908 cites W1965876049 @default.
• W1998640908 cites W1966781760 @default.
• W1998640908 cites W1972138202 @default.
• W1998640908 cites W1991110933 @default.
• W1998640908 cites W1991523775 @default.
• W1998640908 cites W1991854032 @default.
• W1998640908 cites W2002147692 @default.
• W1998640908 cites W2004452444 @default.
• W1998640908 cites W2005073478 @default.
• W1998640908 cites W2006134426 @default.
• W1998640908 cites W2006192061 @default.
• W1998640908 cites W2009590659 @default.
• W1998640908 cites W2016439613 @default.
• W1998640908 cites W2018006954 @default.
• W1998640908 cites W2023577097 @default.
• W1998640908 cites W2027035874 @default.
• W1998640908 cites W2028813694 @default.
• W1998640908 cites W2031964168 @default.
• W1998640908 cites W2032853143 @default.
• W1998640908 cites W2034299708 @default.
• W1998640908 cites W2040738425 @default.
• W1998640908 cites W2042905169 @default.
• W1998640908 cites W2047669024 @default.
• W1998640908 cites W2049504302 @default.
• W1998640908 cites W2050957868 @default.
• W1998640908 cites W2056897249 @default.
• W1998640908 cites W2059440739 @default.
• W1998640908 cites W2062894160 @default.
• W1998640908 cites W2067151543 @default.
• W1998640908 cites W2072372914 @default.
• W1998640908 cites W2074455942 @default.
• W1998640908 cites W2077962784 @default.
• W1998640908 cites W2079168787 @default.
• W1998640908 cites W2081342821 @default.
• W1998640908 cites W2087995428 @default.
• W1998640908 cites W2088693148 @default.
• W1998640908 cites W2089618587 @default.
• W1998640908 cites W2094364221 @default.
• W1998640908 cites W2097853280 @default.
• W1998640908 cites W2099203729 @default.
• W1998640908 cites W2100289006 @default.
• W1998640908 cites W2105193974 @default.
• W1998640908 cites W2106401943 @default.
• W1998640908 cites W2107866321 @default.
• W1998640908 cites W2114421116 @default.
• W1998640908 cites W2118185751 @default.
• W1998640908 cites W2119693327 @default.
• W1998640908 cites W2120646148 @default.
• W1998640908 cites W2120881407 @default.
• W1998640908 cites W2130682733 @default.
• W1998640908 cites W2147553897 @default.
• W1998640908 cites W2148533818 @default.
• W1998640908 cites W2149472608 @default.
• W1998640908 cites W2161726811 @default.
• W1998640908 cites W2162434225 @default.
• W1998640908 cites W2162685411 @default.
• W1998640908 cites W2162720882 @default.
• W1998640908 cites W2162993954 @default.
• W1998640908 cites W2163171275 @default.
• W1998640908 cites W2165445035 @default.
• W1998640908 cites W2165639753 @default.
• W1998640908 cites W2177743694 @default.
• W1998640908 cites W28039676 @default.
• W1998640908 cites W4293247451 @default.
• W1998640908 doi "https://doi.org/10.1021/bi201110h" @default.
• W1998640908 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22112013" @default.
• W1998640908 hasPublicationYear "2011" @default.
• W1998640908 type Work @default.
• W1998640908 sameAs 1998640908 @default.
• W1998640908 citedByCount "24" @default.
• W1998640908 countsByYear W19986409082012 @default.
• W1998640908 countsByYear W19986409082013 @default.
• W1998640908 countsByYear W19986409082014 @default.
• W1998640908 countsByYear W19986409082015 @default.
• W1998640908 countsByYear W19986409082016 @default.
• W1998640908 countsByYear W19986409082017 @default.
• W1998640908 countsByYear W19986409082018 @default.
• W1998640908 countsByYear W19986409082019 @default.
• W1998640908 countsByYear W19986409082020 @default.
• W1998640908 countsByYear W19986409082021 @default.
• W1998640908 countsByYear W19986409082022 @default.
• W1998640908 countsByYear W19986409082023 @default.
• W1998640908 crossrefType "journal-article" @default.
• W1998640908 hasAuthorship W1998640908A5023135666 @default.

• next