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- W1998671247 abstract "Abstract The large monoamine-containing neurone systems in the rat mesencephalon have been characterized and classified by means of microspectrofluorometry in combination with various drug treatments. Previous fluorescence histochemical studies have demonstrated gree fluorescent and yellow fluorescent neurones in this region. The identity of the green fluorescent neurons as catecholamine-containing was confirmed in the present study. The yellow fluorescent neurones were found to be of two distinctly different types. One (the so-called C-type) was identified as 5-hydroxytryptamine (5-HT)-containing. This type had the characteristic 5-HT spectra with the excitation/emission maxima at 315 and 380–390/520–535 nm and showed a rapid photodecomposition. The fluorescence was abolished or strongly reduced by treatment with reserpine or the tryptophan-5-hydroxylase inhibitor, p -chlorophenylalanine, and was markedly increased after treatment with the MAO inhibitor, nialamide. The second yellow fluorescent neurone type (the so-called B-type) had characteristics clearly deviating from those of the 5-HT neurones: the excitation maxima were at 315, 370–380 and 420 nm, the emission maximum was at 495–510 nm, and the photodecomposition rate was rather slow. This B-type of yellow fluorophore was abolished or strongly reduced after the reserpine treatment but was not affected by the treatments with p -chloropheylalanine or nialamide. The B-type cell bodies were found intermingled with the 5-HT cell bodies in the raphe nuclei, most of the B-type cell bodies being localized in the nucleus linearis caudalis and the nucleus medianus raphe (B8) and scattered in a wing-like structure situated lateral to this latter nucleus. The B-type and the 5-HT type of fibres which could, at least partly, be traced back to the raphe nuclei were partially separated in the ventromedial tegmentum. Anteriorly, they ran into the medial forebrain bundle where they intermingled. It is concluded that the p -chlorophenylalanine-resistant B-type neurone is a morphologically and histochemically well-defined and separate entity. The possibility is discussed that a hitherto unknown indolamine is found in the B-type neurone." @default.
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- W1998671247 title "Classification of monoamine neurones in the rat mesencephalon: Distribution of a new monoamine neurone system" @default.
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- W1998671247 doi "https://doi.org/10.1016/0006-8993(71)90324-6" @default.
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