FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1998750499> ?p ?o ?g. }

• W1998750499 endingPage "332" @default.
• W1998750499 startingPage "326" @default.
• W1998750499 abstract "Secreted Frizzled-related protein 1 (sFRP1), as one of most important Wnt antagonists, is frequently silenced by promoter hypermethylation in many types of tumor, including hepatocellular carcinoma (HCC). In this study, we aimed to investigate whether restoration of sFRP1 affected HCC metastatic behavior. sFRP1 mRNA expression and promoter methylation in HCC tissues and cell lines were examined using RT-PCR and methylation-specific PCR (MS-PCR), respectively. sFRP1 protein expression was assessed by Western Blot. We generated stable HCC cell line restoration of sFRP1 in HepG2 cells, which naturally do not express detectable sFRP1 mRNA. The effects of exogenous sFRP1 on HepG2 cell invasion were investigated using trans-well assay. Also the effects of sFRP1 re-expression on the β-catenin/T-cell factor-dependent transcription activity was measured by luciferase assay.sFRP1 promoter methylation was frequently observed in HCC tissues (60%) and cell lines (75%). All samples with sFRP1 methylation showed down-regulation of sFRP1 expression in HCC cell lines. Demethylation treatment with 5-aza-20-deoxycytidine in HCC cells restored sFRP1 expression. Restoration of sFRP1 substantially impaired the invasive potentials of HepG2 cells. Moreover, exogenous sFRP1 caused significant decrease of β-catenin/T-cell factor-dependent transcription activity.These findings demonstrate that sFRP1 silencing due to promoter hypermethylation is a major event during tumorigenesis. sFRP1 is also a negative modulator of canonical Wnt signaling, which could contribute to metastasis in HCC progression, thus providing a possible therapeutic strategy against HCC." @default.
• W1998750499 created "2016-06-24" @default.
• W1998750499 creator A5008754375 @default.
• W1998750499 creator A5015491676 @default.
• W1998750499 creator A5020325989 @default.
• W1998750499 creator A5056770057 @default.
• W1998750499 creator A5066935160 @default.
• W1998750499 creator A5091518069 @default.
• W1998750499 date "2012-01-01" @default.
• W1998750499 modified "2023-09-23" @default.
• W1998750499 title "Epigenetic inactivation of the canonical wnt antagonist secreted frizzled-related protein 1 in hepatocellular carcinoma cells" @default.
• W1998750499 cites W1522000767 @default.
• W1998750499 cites W1970635495 @default.
• W1998750499 cites W1973211134 @default.
• W1998750499 cites W1975341256 @default.
• W1998750499 cites W1982113232 @default.
• W1998750499 cites W1990739275 @default.
• W1998750499 cites W2001269102 @default.
• W1998750499 cites W2017832646 @default.
• W1998750499 cites W2023329572 @default.
• W1998750499 cites W2039621565 @default.
• W1998750499 cites W2041435092 @default.
• W1998750499 cites W2044565426 @default.
• W1998750499 cites W2049181327 @default.
• W1998750499 cites W2051974123 @default.
• W1998750499 cites W2058309856 @default.
• W1998750499 cites W2059509996 @default.
• W1998750499 cites W2063519617 @default.
• W1998750499 cites W2064945386 @default.
• W1998750499 cites W2072439648 @default.
• W1998750499 cites W2089030074 @default.
• W1998750499 cites W2093015940 @default.
• W1998750499 cites W2098837744 @default.
• W1998750499 cites W2099583939 @default.
• W1998750499 cites W2101671356 @default.
• W1998750499 cites W2102854363 @default.
• W1998750499 cites W2106787323 @default.
• W1998750499 cites W2125093701 @default.
• W1998750499 cites W2127280898 @default.
• W1998750499 cites W2158207797 @default.
• W1998750499 cites W2163638510 @default.
• W1998750499 cites W2167638513 @default.
• W1998750499 cites W2400350205 @default.
• W1998750499 cites W2407399555 @default.
• W1998750499 doi "https://doi.org/10.4149/neo_2012_042" @default.
• W1998750499 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22296502" @default.
• W1998750499 hasPublicationYear "2012" @default.
• W1998750499 type Work @default.
• W1998750499 sameAs 1998750499 @default.
• W1998750499 citedByCount "20" @default.
• W1998750499 countsByYear W19987504992013 @default.
• W1998750499 countsByYear W19987504992014 @default.
• W1998750499 countsByYear W19987504992015 @default.
• W1998750499 countsByYear W19987504992016 @default.
• W1998750499 countsByYear W19987504992018 @default.
• W1998750499 countsByYear W19987504992020 @default.
• W1998750499 countsByYear W19987504992021 @default.
• W1998750499 countsByYear W19987504992022 @default.
• W1998750499 crossrefType "journal-article" @default.
• W1998750499 hasAuthorship W1998750499A5008754375 @default.
• W1998750499 hasAuthorship W1998750499A5015491676 @default.
• W1998750499 hasAuthorship W1998750499A5020325989 @default.
• W1998750499 hasAuthorship W1998750499A5056770057 @default.
• W1998750499 hasAuthorship W1998750499A5066935160 @default.
• W1998750499 hasAuthorship W1998750499A5091518069 @default.
• W1998750499 hasBestOaLocation W19987504991 @default.
• W1998750499 hasConcept C104317684 @default.
• W1998750499 hasConcept C119056186 @default.
• W1998750499 hasConcept C121608353 @default.
• W1998750499 hasConcept C137620995 @default.
• W1998750499 hasConcept C150194340 @default.
• W1998750499 hasConcept C153911025 @default.
• W1998750499 hasConcept C190727270 @default.
• W1998750499 hasConcept C33288867 @default.
• W1998750499 hasConcept C41091548 @default.
• W1998750499 hasConcept C502942594 @default.
• W1998750499 hasConcept C54355233 @default.
• W1998750499 hasConcept C555283112 @default.
• W1998750499 hasConcept C62478195 @default.
• W1998750499 hasConcept C81885089 @default.
• W1998750499 hasConcept C86803240 @default.
• W1998750499 hasConcept C95444343 @default.
• W1998750499 hasConceptScore W1998750499C104317684 @default.
• W1998750499 hasConceptScore W1998750499C119056186 @default.
• W1998750499 hasConceptScore W1998750499C121608353 @default.
• W1998750499 hasConceptScore W1998750499C137620995 @default.
• W1998750499 hasConceptScore W1998750499C150194340 @default.
• W1998750499 hasConceptScore W1998750499C153911025 @default.
• W1998750499 hasConceptScore W1998750499C190727270 @default.
• W1998750499 hasConceptScore W1998750499C33288867 @default.
• W1998750499 hasConceptScore W1998750499C41091548 @default.
• W1998750499 hasConceptScore W1998750499C502942594 @default.
• W1998750499 hasConceptScore W1998750499C54355233 @default.
• W1998750499 hasConceptScore W1998750499C555283112 @default.
• W1998750499 hasConceptScore W1998750499C62478195 @default.
• W1998750499 hasConceptScore W1998750499C81885089 @default.
• W1998750499 hasConceptScore W1998750499C86803240 @default.
• W1998750499 hasConceptScore W1998750499C95444343 @default.

• next