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• W199875565 abstract "Little is known about the function of genes expressed in oil palm vegetativemeristem tissues, which are expressed during transition from vegetative toreproductive growth, and those expressed during the formation of abnormalinflorescence. This study was aimed at isolating sufficiently large numbers ofexpressed sequence tags (ESTs) from an oil palm vegetative meristem cDNA libraryso that the expression of genes in this tissue could be studied. The genes (or ESTs)that were specifically expressed in vegetative meristems at early stages of normalinflorescence meristem development, and in abnormal inflorescence meristems wereisolated to study the transition from vegetative to reproductive growth and theformation of floral abnormalities from clonal palms.A random EST approach has been used to obtain vast amounts of genes frommany organisms. However, the random EST approach may result in the isolation ofclones that are highly repeated in proportion to their abundance in the mRNA population of the appropriate tissues. Therefore, cold-plaque screemng andsuppression subtractive hybridization (SSH) techniques were employed in the ESTapproach to isolate as much unique transcripts as possible in this study.Based on the EST collections made from the vegetative meristem tissues,1088 ESTs were isolated. The redundancy of the ESTs was reduced to about 18.9%where 81.1% out of 1088 oil palm vegetative meristem ESTs were unique due to theuse of the cold-plaque screening approach. Classification of the putative function ofESTs provides an idea of the type of genes expressed in the vegetative meristemtissue. The expressed genes range from housekeeping genes to genes related tophotosynthesis. About 44% of the ESTs were unknown and were not characterized inother species.Stage specific expressed genes were isolated from vegetative meristem,inflorescence meristem, normal and abnormal inflorescence meristem subtractionlibraries. About 601 contigs were identified from these four subtraction libraries.More than 57% of the ESTs encoded unknown functions. Through the use of digitaldifferential display calculations, the ESTs were expressed in a tissue specific manner.Reverse northern analysis confirmed that the the majority of the EST -contigs weretissue specific.This study reveals that the apical meristem devotes more cellular activity tothe biosynthesis of cellular components than to photosynthesis which is predictedfrom the EST analysis and physiological experiments conducted on plants. This studyalso reveals profound changes in gene expression that are involved in the transitionfrom the vegetative meristem to an inflorescence meristem that is driven by the actionof a series of genes expressed in a stage-specific manner. Significant differences ingene expression between normal and abnormal inflorescence meristems could not bedetected with this EST approach. Transient changes in gene expression or epigeneticphenomenon have been proposed to explain the molecular basis of abnormal floraldevelopment." @default.
• W199875565 created "2016-06-24" @default.
• W199875565 creator A5053005031 @default.
• W199875565 date "2003-01-01" @default.
• W199875565 modified "2023-09-24" @default.
• W199875565 title "Characterization of Expressed Genes Isolated From Oil Palm Vegetative, Normal and Abnormal Inflorescence Meristems Using EST Approach" @default.
• W199875565 hasPublicationYear "2003" @default.
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