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• W1998781683 abstract "Ceropegiaevansii McCann (family: Asclepiadaceae), a critically endangered plant of Western Ghats has acquired significant importance due to its medicinal implications, edible tubers, and ornamental flowers. This study deals with the optimization of axillary bud proliferation using nodal explants followed by genetic stability analysis of regenerants. Maximum number of shoots (11.6 ± 1.1) was observed on the Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (4.0 mg/l) and indole-3-acetic acid (0.3 mg/l) with 85% shoot multiplication frequency. In vitro-grown shoots were rooted best in 1/2 MS medium supplemented with indole-3-butyric acid (1.0 mg/l) with an average of 10.3 ± 0.9 roots per shoot and 92% rooting frequency. Plantlets were acclimatized best (90%) in a mixture of sterile soil, sand, and coco peat (1:2:1). Micropropagated plants were subjected to random amplified polymorphic DNA and inter simple sequence repeat markers analyses. Collectively, 759 bands were generated which were monomorphic and similar to the mother plant. Findings of this study are the first report on micropropagation and assessment of genetic stability of micropropagated plantlets in C. evansii which suggests that axillary shoot proliferation can safely be used as an effective tool for propagation and conservation of C. evansii." @default.
• W1998781683 created "2016-06-24" @default.
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• W1998781683 date "2013-11-06" @default.
• W1998781683 modified "2023-09-23" @default.
• W1998781683 title "Highly efficient<i>in vitro</i>proliferation and genetic stability analysis of micropropagated<i>Ceropegia</i><i>evansii</i>by RAPD and ISSR markers: A critically endangered plant of Western Ghats" @default.
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• W1998781683 doi "https://doi.org/10.1080/11263504.2013.853700" @default.
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