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• W1998863653 abstract "Abstract In cortical neurons cultured for 3 or 9 days in vitro (DIV), exposure to hydrogen peroxide (H 2 O 2 ) led to a marked decrease in cell viability in a concentration‐dependent manner at a concentration range of 10 µ m to 1 m m irrespective of the duration between 6 and 24 h. However, H 2 O 2 was more potent in decreasing cellular viability in cortical neurons cultured for 9 DIV than in those for 3 DIV. Pyruvate was effective in preventing the neuronal cell death at 1 m m even when added 1–3 h after the addition of H 2 O 2 . Semi‐quantitative RT–PCR and western blotting analyses revealed significantly higher expression of both mRNA and protein for a particular monocarboxylate transporter (MCT) in neurons cultured for 9 DIV than in those for 3 DIV. A specific inhibitor of MCT significantly attenuated the neuroprotection by pyruvate in neurons cultured for 9 DIV, without markedly affecting that in neurons cultured for 3 DIV. These results suggest that vulnerability to H 2 O 2 may at least in part involve expression of particular MCT isoforms responsible for the bi‐directional transport of pyruvate across cell surfaces in cultured rat cortical neurons." @default.
• W1998863653 created "2016-06-24" @default.
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• W1998863653 date "2005-02-17" @default.
• W1998863653 modified "2023-10-16" @default.
• W1998863653 title "Protection by exogenous pyruvate through a mechanism related to monocarboxylate transporters against cell death induced by hydrogen peroxide in cultured rat cortical neurons" @default.
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• W1998863653 doi "https://doi.org/10.1111/j.1471-4159.2005.02999.x" @default.
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