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- W1998897281 endingPage "360" @default.
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- W1998897281 abstract "Mesoporous materials as support for immobilized enzymes have been explored extensively during the last two decades, primarily not only for biocatalysis applications, but also for biosensing, biofuels and enzyme-controlled drug delivery. The activity of the immobilized enzymes inside the pores is often different compared to that of the free enzymes, and an important challenge is to understand how the immobilization affects the enzymes in order to design immobilization conditions that lead to optimal enzyme activity. This review summarizes methods that can be used to understand how material properties can be linked to changes in enzyme activity. Real-time monitoring of the immobilization process and techniques that demonstrate that the enzymes are located inside the pores is discussed by contrasting them to the common practice of indirectly measuring the depletion of the protein concentration or enzyme activity in the surrounding bulk phase. We propose that pore filling (pore volume fraction occupied by proteins) is the best standard for comparing the amount of immobilized enzymes at the molecular level, and present equations to calculate pore filling from the more commonly reported immobilized mass. Methods to detect changes in enzyme structure upon immobilization and to study the microenvironment inside the pores are discussed in detail. Combining the knowledge generated from these methodologies should aid in rationally designing biocatalyst based on enzymes immobilized in mesoporous materials." @default.
- W1998897281 created "2016-06-24" @default.
- W1998897281 creator A5010834156 @default.
- W1998897281 creator A5024691627 @default.
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- W1998897281 creator A5045121003 @default.
- W1998897281 creator A5046281470 @default.
- W1998897281 creator A5090398782 @default.
- W1998897281 date "2014-03-01" @default.
- W1998897281 modified "2023-09-29" @default.
- W1998897281 title "Enzymes immobilized in mesoporous silica: A physical–chemical perspective" @default.
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