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- W1998965722 abstract "Abstract Background The androgen receptor (AR) is a member of the nuclear receptor (NR) superfamily of ligand-inducible DNA transcription factors, and is the major mediator of male sexual development, prostate growth and the pathogenesis of prostate cancer. Cell and gene specific regulation by the AR is determined by availability of and interaction with sets of key accessory cofactors. Ski-interacting protein (SKIP; SNW1, NCOA62) is a cofactor shown to interact with several NRs and a diverse range of other transcription factors. Interestingly, SKIP as part of the spliceosome is thought to link mRNA splicing with transcription. SKIP has not been previously shown to interact with the AR. Results The aim of this study was to investigate whether SKIP interacts with the AR and modulates AR-dependent transcription. Here, we show by co-immunoprecipitation experiments that SKIP is in a complex with the AR. Moreover, SKIP increased 5α-dihydrotestosterone (DHT) induced N-terminal/C-terminal AR interaction from 12-fold to almost 300-fold in a two-hybrid assay, and enhanced AR ligand-independent AF-1 transactivation. SKIP augmented ligand- and AR-dependent transactivation in PC3 prostate cancer cells. Live-cell imaging revealed a fast (half-time=129 s) translocation of AR from the cytoplasm to the nucleus upon DHT-stimulation. Förster resonance energy transfer (FRET) experiments suggest a direct AR-SKIP interaction in the nucleus upon translocation. Conclusions Our results suggest that SKIP interacts with AR in the nucleus and enhances AR-dependent transactivation and N/C-interaction supporting a role for SKIP as an AR co-factor." @default.
- W1998965722 created "2016-06-24" @default.
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- W1998965722 date "2013-04-08" @default.
- W1998965722 modified "2023-10-16" @default.
- W1998965722 title "Ski-interacting protein (SKIP) interacts with androgen receptor in the nucleus and modulates androgen-dependent transcription" @default.
- W1998965722 cites W1495912963 @default.
- W1998965722 cites W1723187988 @default.
- W1998965722 cites W1969201427 @default.
- W1998965722 cites W1971227936 @default.
- W1998965722 cites W1971955021 @default.
- W1998965722 cites W1971960644 @default.
- W1998965722 cites W1973331161 @default.
- W1998965722 cites W1976003215 @default.
- W1998965722 cites W1976278905 @default.
- W1998965722 cites W1977186059 @default.
- W1998965722 cites W1978884176 @default.
- W1998965722 cites W1986320198 @default.
- W1998965722 cites W1986879525 @default.
- W1998965722 cites W1995021282 @default.
- W1998965722 cites W1996880521 @default.
- W1998965722 cites W1997998562 @default.
- W1998965722 cites W1998383661 @default.
- W1998965722 cites W1998944669 @default.
- W1998965722 cites W2004824571 @default.
- W1998965722 cites W2005664674 @default.
- W1998965722 cites W2010052865 @default.
- W1998965722 cites W2019757555 @default.
- W1998965722 cites W2021051883 @default.
- W1998965722 cites W2021999304 @default.
- W1998965722 cites W2026561146 @default.
- W1998965722 cites W2030640098 @default.
- W1998965722 cites W2037427470 @default.
- W1998965722 cites W2040711515 @default.
- W1998965722 cites W2041170006 @default.
- W1998965722 cites W2054191752 @default.
- W1998965722 cites W2064293531 @default.
- W1998965722 cites W2068924803 @default.
- W1998965722 cites W2071790247 @default.
- W1998965722 cites W2074424265 @default.
- W1998965722 cites W2078342226 @default.
- W1998965722 cites W2081557764 @default.
- W1998965722 cites W2082610063 @default.
- W1998965722 cites W2086908959 @default.
- W1998965722 cites W2089518544 @default.
- W1998965722 cites W2090823563 @default.
- W1998965722 cites W2095649647 @default.
- W1998965722 cites W2099681868 @default.
- W1998965722 cites W2099856732 @default.
- W1998965722 cites W2109992434 @default.
- W1998965722 cites W2123792331 @default.
- W1998965722 cites W2124125820 @default.
- W1998965722 cites W2125069527 @default.
- W1998965722 cites W2127885441 @default.
- W1998965722 cites W2130277194 @default.
- W1998965722 cites W2130538948 @default.
- W1998965722 cites W2131848867 @default.
- W1998965722 cites W2133946422 @default.
- W1998965722 cites W2153161471 @default.
- W1998965722 cites W2154095897 @default.
- W1998965722 cites W2154693798 @default.
- W1998965722 cites W2161123101 @default.
- W1998965722 cites W2164472870 @default.
- W1998965722 cites W2168779308 @default.
- W1998965722 cites W2315656172 @default.
- W1998965722 cites W2605614532 @default.
- W1998965722 cites W4211238694 @default.
- W1998965722 cites W4231215882 @default.
- W1998965722 cites W4241870034 @default.
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- W1998965722 doi "https://doi.org/10.1186/1471-2091-14-10" @default.
- W1998965722 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3668167" @default.
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