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- W1998989968 abstract "Candida milleri, together with Candida humilis, is the most representative yeast species found in type I sourdough ecosystems. In this work, comparison of the ITS region and the D1/D2 domain of 26S rDNA gene partial sequences, karyotyping, mtDNA-RFLP analysis, Intron Splice Site dispersion (ISS-PCR) and (GTG)5 microsatellite analyses, assimilation test of different carbohydrates, and metabolome assessment by FT-IR analysis, were investigated in seventeen strains isolated from four different companies as well as in type strains CBS6897T and CBS5658T. Most isolates were ascribed to C. milleri, even if a strong relatedness was confirmed with C. humilis as well, particularly for three strains. Genetic characterization showed a high degree of intraspecific polymorphism since 12 different genotypes were discriminated. The number of chromosomes varied from 9 to 13 and their size ranged from less than 0.3 to over 2 Mbp. Phenotypic traits let to recognize 9 different profiles of carbon sources assimilation. FT-IR spectra from yeast cells cultivated in different media and collected at different growth phases revealed a diversity of behaviour among strains in accordance with the results of PCR-based fingerprinting. A clear evidence of the polymorphic status of C. milleri species is provided thus representing an important feature for the development of technological applications in bakery industries." @default.
- W1998989968 created "2016-06-24" @default.
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- W1998989968 date "2014-09-01" @default.
- W1998989968 modified "2023-09-26" @default.
- W1998989968 title "Candida milleri species reveals intraspecific genetic and metabolic polymorphisms" @default.
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- W1998989968 doi "https://doi.org/10.1016/j.fm.2014.02.011" @default.
- W1998989968 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24929720" @default.
- W1998989968 hasPublicationYear "2014" @default.
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