Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999006912> ?p ?o ?g. }
- W1999006912 endingPage "3516" @default.
- W1999006912 startingPage "3500" @default.
- W1999006912 abstract "The opium poppy (Papaver somniferum) belongs to the group of latex-containing plants. Latex is the milky-like fluid within laticifer cells. In this study, poppy latex was analyzed with respect to ultrastructure, alkaloid, and protein content. The main goal of this project was the examination of the proteins by two-dimensional gel electrophoresis. In a proteomics approach, we investigated two main fractions of the latex, namely the cytosolic serum and the sedimented fraction containing the alkaloid-accumulating vesicles. Of the serum, representing the protein-rich part of the latex, 75 spots were analyzed by internal peptide microsequencing, followed by a database searching. For 69 proteins a function could be assigned due to homology to known proteins, whereas six spots could not be identified. Furthermore, codeinone reductase, a representative of the specific enzyme system in morphine biosynthesis, could be detected within the cytosolic serum fraction. In the vesicle-containing pellet, 23 protein spots were analyzed. An attempt was also made to separate the vesicle pellet by density centrifugation, followed by investigation of the alkaloid content, ultrastructure, and protein pattern. This study describes the first database of soluble proteins present in the latex of P. somniferum" @default.
- W1999006912 created "2016-06-24" @default.
- W1999006912 creator A5040083768 @default.
- W1999006912 creator A5041519853 @default.
- W1999006912 creator A5041718533 @default.
- W1999006912 creator A5086595920 @default.
- W1999006912 date "2000-10-01" @default.
- W1999006912 modified "2023-10-17" @default.
- W1999006912 title "Characterization of proteins in latex of the opium poppy (Papaver somniferum) using two-dimensional gel electrophoresis and microsequencing" @default.
- W1999006912 cites W1235761713 @default.
- W1999006912 cites W1538113207 @default.
- W1999006912 cites W1563997058 @default.
- W1999006912 cites W1606423922 @default.
- W1999006912 cites W1752750900 @default.
- W1999006912 cites W1795884996 @default.
- W1999006912 cites W1801752440 @default.
- W1999006912 cites W1804612838 @default.
- W1999006912 cites W1964435550 @default.
- W1999006912 cites W1964525381 @default.
- W1999006912 cites W1965232578 @default.
- W1999006912 cites W1966947574 @default.
- W1999006912 cites W1970082049 @default.
- W1999006912 cites W1971068216 @default.
- W1999006912 cites W1973028688 @default.
- W1999006912 cites W1976449821 @default.
- W1999006912 cites W1976821563 @default.
- W1999006912 cites W1980166477 @default.
- W1999006912 cites W1986814752 @default.
- W1999006912 cites W1987295841 @default.
- W1999006912 cites W1987871659 @default.
- W1999006912 cites W1989883631 @default.
- W1999006912 cites W1990625934 @default.
- W1999006912 cites W1992371295 @default.
- W1999006912 cites W1992630550 @default.
- W1999006912 cites W1994739715 @default.
- W1999006912 cites W1995247882 @default.
- W1999006912 cites W1999493965 @default.
- W1999006912 cites W2004513287 @default.
- W1999006912 cites W2005030605 @default.
- W1999006912 cites W2006497539 @default.
- W1999006912 cites W2013504443 @default.
- W1999006912 cites W2016426511 @default.
- W1999006912 cites W2017810508 @default.
- W1999006912 cites W2022966294 @default.
- W1999006912 cites W2029553161 @default.
- W1999006912 cites W2034872394 @default.
- W1999006912 cites W2035021778 @default.
- W1999006912 cites W2038179253 @default.
- W1999006912 cites W2041671226 @default.
- W1999006912 cites W2043158316 @default.
- W1999006912 cites W2044117564 @default.
- W1999006912 cites W2048760229 @default.
- W1999006912 cites W2049686415 @default.
- W1999006912 cites W2051658789 @default.
- W1999006912 cites W2058128222 @default.
- W1999006912 cites W2058676872 @default.
- W1999006912 cites W2061846873 @default.
- W1999006912 cites W2062931354 @default.
- W1999006912 cites W2063044483 @default.
- W1999006912 cites W2063549906 @default.
- W1999006912 cites W2070161151 @default.
- W1999006912 cites W2072759178 @default.
- W1999006912 cites W2074943127 @default.
- W1999006912 cites W2074978715 @default.
- W1999006912 cites W2075994511 @default.
- W1999006912 cites W2079038249 @default.
- W1999006912 cites W2085375492 @default.
- W1999006912 cites W2087042268 @default.
- W1999006912 cites W2087426415 @default.
- W1999006912 cites W2088272759 @default.
- W1999006912 cites W2091994499 @default.
- W1999006912 cites W2093100151 @default.
- W1999006912 cites W2096275788 @default.
- W1999006912 cites W2099265131 @default.
- W1999006912 cites W2101218484 @default.
- W1999006912 cites W2113964973 @default.
- W1999006912 cites W2120689665 @default.
- W1999006912 cites W2121418514 @default.
- W1999006912 cites W2126425082 @default.
- W1999006912 cites W2129683367 @default.
- W1999006912 cites W2130669719 @default.
- W1999006912 cites W2130783274 @default.
- W1999006912 cites W2134245322 @default.
- W1999006912 cites W2153247725 @default.
- W1999006912 cites W2156934810 @default.
- W1999006912 cites W2157510518 @default.
- W1999006912 cites W2164044418 @default.
- W1999006912 cites W2166460120 @default.
- W1999006912 cites W2243694838 @default.
- W1999006912 cites W2320309435 @default.
- W1999006912 cites W2510185479 @default.
- W1999006912 cites W4240061572 @default.
- W1999006912 cites W4246453702 @default.
- W1999006912 cites W4250241753 @default.
- W1999006912 cites W4293247451 @default.
- W1999006912 cites W4296926797 @default.
- W1999006912 doi "https://doi.org/10.1002/1522-2683(20001001)21:16<3500::aid-elps3500>3.0.co;2-o" @default.
- W1999006912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11079569" @default.