FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999013973> ?p ?o ?g. }

• W1999013973 endingPage "273" @default.
• W1999013973 startingPage "268" @default.
• W1999013973 abstract "Hyperglycemia increases the production of reactive oxygen species (ROS). NAD(P)H oxidase, producing superoxide anion, is the main source of ROS in diabetic podocytes and their production contributes to the development of diabetic nephropathy. We have investigated the effect of an antidiabetic drug, metformin on the production of superoxide anion in cultured podocytes and attempted to elucidate underlying mechanisms. The experiments were performed in normal (NG, 5.6 mM) and high (HG, 30 mM) glucose concentration. Overall ROS production was measured by fluorescence of a DCF probe. Activity of NAD(P)H oxidase was measured by chemiluminescence method. The AMP-dependent kinase (AMPK) activity was determined by immunobloting, measuring the ratio of phosphorylated AMPK to total AMPK. Glucose accumulation was measured using 2-deoxy-[1,2-3H]-glucose. ROS production increased by about 27% (187 ± 8 vs. 238 ± 9 arbitrary units AU, P < 0.01) in HG. Metformin (2 mM, 2 h) markedly reduced ROS production by 45% in NG and 60% in HG. Metformin decreased NAD(P)H oxidase activity in NG (36%) and HG (86%). AMPK activity was increased by metformin in NG and HG (from 0.58 ± 0.07 to. 0.99 ± 0.06, and from 0.53 ± 0.03 to 0.64 ± 0.03; P < 0.05). The effects of metformin on the activities of NAD(P)H oxidase and AMPK were abolished in the presence of AMPK inhibitor, compound C. We have shown that metformin decreases production of ROS through reduction of NAD(P)H oxidase activity. We also have demonstrated relationship between activity of NAD(P)H oxidase and AMPK." @default.
• W1999013973 created "2016-06-24" @default.
• W1999013973 creator A5027060523 @default.
• W1999013973 creator A5029103435 @default.
• W1999013973 creator A5056313940 @default.
• W1999013973 creator A5074229388 @default.
• W1999013973 creator A5081094521 @default.
• W1999013973 creator A5087903728 @default.
• W1999013973 date "2010-03-01" @default.
• W1999013973 modified "2023-10-13" @default.
• W1999013973 title "Metformin induces suppression of NAD(P)H oxidase activity in podocytes" @default.
• W1999013973 cites W1921965616 @default.
• W1999013973 cites W1977424733 @default.
• W1999013973 cites W1977913875 @default.
• W1999013973 cites W1978763812 @default.
• W1999013973 cites W1990473965 @default.
• W1999013973 cites W2006008851 @default.
• W1999013973 cites W2010238890 @default.
• W1999013973 cites W2012258975 @default.
• W1999013973 cites W2019354525 @default.
• W1999013973 cites W2020763506 @default.
• W1999013973 cites W2028011534 @default.
• W1999013973 cites W2029818480 @default.
• W1999013973 cites W2031800834 @default.
• W1999013973 cites W2040394896 @default.
• W1999013973 cites W2053448161 @default.
• W1999013973 cites W2059407166 @default.
• W1999013973 cites W2076569716 @default.
• W1999013973 cites W2079837239 @default.
• W1999013973 cites W2080505183 @default.
• W1999013973 cites W2085253569 @default.
• W1999013973 cites W2094719535 @default.
• W1999013973 cites W2100829672 @default.
• W1999013973 cites W2100990796 @default.
• W1999013973 cites W2102235083 @default.
• W1999013973 cites W2103676852 @default.
• W1999013973 cites W2104989112 @default.
• W1999013973 cites W2109668012 @default.
• W1999013973 cites W2110676635 @default.
• W1999013973 cites W2111478784 @default.
• W1999013973 cites W2113053818 @default.
• W1999013973 cites W2115724806 @default.
• W1999013973 cites W2138226825 @default.
• W1999013973 cites W2142693924 @default.
• W1999013973 cites W2146242381 @default.
• W1999013973 cites W2156913795 @default.
• W1999013973 cites W2159235836 @default.
• W1999013973 cites W2159742843 @default.
• W1999013973 cites W2471605942 @default.
• W1999013973 cites W4231555469 @default.
• W1999013973 doi "https://doi.org/10.1016/j.bbrc.2010.01.119" @default.
• W1999013973 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20123087" @default.
• W1999013973 hasPublicationYear "2010" @default.
• W1999013973 type Work @default.
• W1999013973 sameAs 1999013973 @default.
• W1999013973 citedByCount "116" @default.
• W1999013973 countsByYear W19990139732012 @default.
• W1999013973 countsByYear W19990139732013 @default.
• W1999013973 countsByYear W19990139732014 @default.
• W1999013973 countsByYear W19990139732015 @default.
• W1999013973 countsByYear W19990139732016 @default.
• W1999013973 countsByYear W19990139732017 @default.
• W1999013973 countsByYear W19990139732018 @default.
• W1999013973 countsByYear W19990139732019 @default.
• W1999013973 countsByYear W19990139732020 @default.
• W1999013973 countsByYear W19990139732021 @default.
• W1999013973 countsByYear W19990139732022 @default.
• W1999013973 countsByYear W19990139732023 @default.
• W1999013973 crossrefType "journal-article" @default.
• W1999013973 hasAuthorship W1999013973A5027060523 @default.
• W1999013973 hasAuthorship W1999013973A5029103435 @default.
• W1999013973 hasAuthorship W1999013973A5056313940 @default.
• W1999013973 hasAuthorship W1999013973A5074229388 @default.
• W1999013973 hasAuthorship W1999013973A5081094521 @default.
• W1999013973 hasAuthorship W1999013973A5087903728 @default.
• W1999013973 hasConcept C11960822 @default.
• W1999013973 hasConcept C126322002 @default.
• W1999013973 hasConcept C134018914 @default.
• W1999013973 hasConcept C181199279 @default.
• W1999013973 hasConcept C185592680 @default.
• W1999013973 hasConcept C2779719074 @default.
• W1999013973 hasConcept C2780124434 @default.
• W1999013973 hasConcept C2780323712 @default.
• W1999013973 hasConcept C2780795997 @default.
• W1999013973 hasConcept C2781252958 @default.
• W1999013973 hasConcept C38485361 @default.
• W1999013973 hasConcept C48349386 @default.
• W1999013973 hasConcept C55493867 @default.
• W1999013973 hasConcept C555293320 @default.
• W1999013973 hasConcept C71924100 @default.
• W1999013973 hasConcept C75520062 @default.
• W1999013973 hasConcept C86803240 @default.
• W1999013973 hasConcept C97029542 @default.
• W1999013973 hasConceptScore W1999013973C11960822 @default.
• W1999013973 hasConceptScore W1999013973C126322002 @default.
• W1999013973 hasConceptScore W1999013973C134018914 @default.
• W1999013973 hasConceptScore W1999013973C181199279 @default.
• W1999013973 hasConceptScore W1999013973C185592680 @default.

• next