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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999062010> ?p ?o ?g. }

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• W1999062010 abstract "We have cloned in Escherichia coli and sequenced a 1489-bp DNA fragment conferring resistance to kanamycin and originating from the streptococcal plasmid pJH1. The resistance gene was located by analysis of the initiation and termination codons in an open reading frame (ORF) of 792 bp. The deduced gene product, a 3'5''-aminoglycoside phosphotransferase of type III, has an Mr of 29 200. Comparison of its amino acid sequence with those of type I (Oka et al., 1981) and type II (Beck et al., 1982) 3' phosphotransferases, from transposable elements Tn903 and Tn5, respectively, indicated a statistically significant structural relationship between these enzymes from phylogenetically remote bacterial genera. The degrees of homology observed indicate that phosphotransferase type III and type I genes have diverged from a common ancestor and that the phosphotransferase type II gene has emerged more recently from the type I evolutionary pathway." @default.
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• W1999062010 date "1983-09-01" @default.
• W1999062010 modified "2023-09-30" @default.
• W1999062010 title "Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3'5''-aminoglycoside phosphotransferase type III" @default.
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• W1999062010 doi "https://doi.org/10.1016/0378-1119(83)90022-7" @default.
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