FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999077905> ?p ?o ?g. }

• W1999077905 endingPage "328" @default.
• W1999077905 startingPage "325" @default.
• W1999077905 abstract "Polyphenol oxidase (PPO; EC 1.10.3.2) was isolated from wheat bran by a procedure that included ammonium sulfate fractionation, batch adsorption by DEAE-cellulofine, CM-cellulofine column chromatography, DEAE-cellulofine column chromatography, preparative isoelectric focusing, adsorption on the membrane of a Vivapure Q Maxi H spin column, and heat treatment. These procedures led to 150-fold purification with 4.2% recovery. The PPO was homogeneous by SDS/PAGE. The relative molecular weight of the PPO was estimated to be 37,000 based on its mobility in SDS/PAGE. The isoelectric point of the PPO was 4.4. The K(m) values of the PPO for caffeic acid, chlorogenic acid, pyrocatechol, 4-methyl catechol and l-DOPA as substrates were 0.077, 0.198, 1.176, 1.667 and 4.545 mM. The PPO was strongly inhibited by tropolone. The K(i) value for tropolone is 2.2 x 10(-7) M. The sequence of the 15 N-terminal amino-acid residues was determined to be ATDVRLSIAHQTRFA, which was identical to those of serpin from Triticum aestivum and protein Z from Hordeum vulgare. The PPO strongly inhibited the activity of trypsin, which is an enzyme of serine proteases; 50% inhibition was observed with 1.5 x 10(-7) M PPO. The K(i) value for PPO is 2.3 x 10(-8) M. The wheat bran PPO should be a very important protein for protecting wheat against disease, virus, insect and herbivore damages by both the activities of PPO and protease inhibitor." @default.
• W1999077905 created "2016-06-24" @default.
• W1999077905 creator A5041247017 @default.
• W1999077905 creator A5058609880 @default.
• W1999077905 creator A5085945190 @default.
• W1999077905 date "2008-05-26" @default.
• W1999077905 modified "2023-09-25" @default.
• W1999077905 title "Polyphenol oxidase from wheat bran is a serpin." @default.
• W1999077905 cites W1581723540 @default.
• W1999077905 cites W1841779637 @default.
• W1999077905 cites W1875088677 @default.
• W1999077905 cites W1973495830 @default.
• W1999077905 cites W1996289077 @default.
• W1999077905 cites W2004632484 @default.
• W1999077905 cites W2007454550 @default.
• W1999077905 cites W2012213837 @default.
• W1999077905 cites W2026418762 @default.
• W1999077905 cites W2037607846 @default.
• W1999077905 cites W2037762416 @default.
• W1999077905 cites W2039846210 @default.
• W1999077905 cites W2040641753 @default.
• W1999077905 cites W2054845157 @default.
• W1999077905 cites W2060289384 @default.
• W1999077905 cites W2066493478 @default.
• W1999077905 cites W2066803957 @default.
• W1999077905 cites W2082399163 @default.
• W1999077905 cites W2086396734 @default.
• W1999077905 cites W2100725614 @default.
• W1999077905 cites W2100837269 @default.
• W1999077905 cites W2128017896 @default.
• W1999077905 doi "https://doi.org/10.18388/abp.2008_3079" @default.
• W1999077905 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18506224" @default.
• W1999077905 hasPublicationYear "2008" @default.
• W1999077905 type Work @default.
• W1999077905 sameAs 1999077905 @default.
• W1999077905 citedByCount "15" @default.
• W1999077905 countsByYear W19990779052013 @default.
• W1999077905 countsByYear W19990779052014 @default.
• W1999077905 countsByYear W19990779052015 @default.
• W1999077905 countsByYear W19990779052017 @default.
• W1999077905 countsByYear W19990779052018 @default.
• W1999077905 countsByYear W19990779052019 @default.
• W1999077905 countsByYear W19990779052021 @default.
• W1999077905 crossrefType "journal-article" @default.
• W1999077905 hasAuthorship W1999077905A5041247017 @default.
• W1999077905 hasAuthorship W1999077905A5058609880 @default.
• W1999077905 hasAuthorship W1999077905A5085945190 @default.
• W1999077905 hasBestOaLocation W19990779051 @default.
• W1999077905 hasConcept C162008176 @default.
• W1999077905 hasConcept C178790620 @default.
• W1999077905 hasConcept C181199279 @default.
• W1999077905 hasConcept C185592680 @default.
• W1999077905 hasConcept C206139338 @default.
• W1999077905 hasConcept C2776338311 @default.
• W1999077905 hasConcept C2776350231 @default.
• W1999077905 hasConcept C2776714187 @default.
• W1999077905 hasConcept C2776873940 @default.
• W1999077905 hasConcept C2778004101 @default.
• W1999077905 hasConcept C2778224086 @default.
• W1999077905 hasConcept C2780117898 @default.
• W1999077905 hasConcept C2993665447 @default.
• W1999077905 hasConcept C43617362 @default.
• W1999077905 hasConcept C46757340 @default.
• W1999077905 hasConcept C50476208 @default.
• W1999077905 hasConcept C55493867 @default.
• W1999077905 hasConcept C59822182 @default.
• W1999077905 hasConcept C86803240 @default.
• W1999077905 hasConcept C96294017 @default.
• W1999077905 hasConceptScore W1999077905C162008176 @default.
• W1999077905 hasConceptScore W1999077905C178790620 @default.
• W1999077905 hasConceptScore W1999077905C181199279 @default.
• W1999077905 hasConceptScore W1999077905C185592680 @default.
• W1999077905 hasConceptScore W1999077905C206139338 @default.
• W1999077905 hasConceptScore W1999077905C2776338311 @default.
• W1999077905 hasConceptScore W1999077905C2776350231 @default.
• W1999077905 hasConceptScore W1999077905C2776714187 @default.
• W1999077905 hasConceptScore W1999077905C2776873940 @default.
• W1999077905 hasConceptScore W1999077905C2778004101 @default.
• W1999077905 hasConceptScore W1999077905C2778224086 @default.
• W1999077905 hasConceptScore W1999077905C2780117898 @default.
• W1999077905 hasConceptScore W1999077905C2993665447 @default.
• W1999077905 hasConceptScore W1999077905C43617362 @default.
• W1999077905 hasConceptScore W1999077905C46757340 @default.
• W1999077905 hasConceptScore W1999077905C50476208 @default.
• W1999077905 hasConceptScore W1999077905C55493867 @default.
• W1999077905 hasConceptScore W1999077905C59822182 @default.
• W1999077905 hasConceptScore W1999077905C86803240 @default.
• W1999077905 hasConceptScore W1999077905C96294017 @default.
• W1999077905 hasIssue "2" @default.
• W1999077905 hasLocation W19990779051 @default.
• W1999077905 hasLocation W19990779052 @default.
• W1999077905 hasOpenAccess W1999077905 @default.
• W1999077905 hasPrimaryLocation W19990779051 @default.
• W1999077905 hasRelatedWork W1967347272 @default.
• W1999077905 hasRelatedWork W1982161946 @default.
• W1999077905 hasRelatedWork W1999077905 @default.
• W1999077905 hasRelatedWork W2062411795 @default.
• W1999077905 hasRelatedWork W2064024213 @default.

• next