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- W1999108329 abstract "Abstract 3H-RNA was synthesized using Escherichia coli RNA polymerase with supercoiled polyoma virus DNA as template. DNA was isolated from mouse embryo cells grown in the presence of 5-fluorodeoxyuridine and 5-bromodeoxyuridine and subsequently infected with polyoma virus in the presence of 5-fluorodeoxyuridine alone for 14 hr. The “heavy” mouse DNA was fractionated on CsCl gradients to remove the infecting light virus DNA, and was then hybridized with polyoma 3H-RNA, From the level of radioactivity hybridized, it was estimated that 8–15 polyoma genome equivalents were integrated per cell. Since integration occurs in this lytic infection, the parallel between lytic and non-lytic infections may be more extensive than hitherto suspected." @default.
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- W1999108329 date "1972-04-01" @default.
- W1999108329 modified "2023-09-25" @default.
- W1999108329 title "Evidence for the integration of polyoma virus DNA in a lytic system" @default.
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- W1999108329 doi "https://doi.org/10.1016/0042-6822(72)90113-4" @default.
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