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- W1999118410 abstract "In the dogfish testis, the cystic arrangement and polarization of germ cell stages make it possible to observe all stages of spermatogenesis in a single transverse section. By taking advantage of the zonation of this organ, we have used suppressive subtractive libraries construction, real-time PCR, and in situ hybridization to identify 32 dogfish genes showing differential expressions during spermatogenesis. These include homologs of genes already known to be expressed in the vertebrate testis, but found here to be specifically expressed either in pre-meiotic and/or meiotic zones (ribosomal protein S8, high-mobility group box 3, ubiquitin carboxyl-terminal esterase L3, 20β-hydroxysteroid dehydrogenase, or cyclophilin B) or in post-meiotic zone (speriolin, Soggy, zinc finger protein 474, calreticulin, or phospholipase c-ζ). We also report, for the first time, testis-specific expression patterns for dogfish genes coding for A-kinase anchor protein 5, ring finger protein 152, or F-box only protein 7. Finally, the study highlights the differential expression of new sequences whose identity remains to be assessed. This study provides the first molecular characterization of spermatogenesis in a chondrichthyan, a key species to gain insight into the evolution of this process in gnathostomes." @default.
- W1999118410 created "2016-06-24" @default.
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- W1999118410 date "2010-07-01" @default.
- W1999118410 modified "2023-10-11" @default.
- W1999118410 title "Stage-specific gene expression during spermatogenesis in the dogfish (Scyliorhinus canicula)" @default.
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- W1999118410 doi "https://doi.org/10.1530/rep-10-0021" @default.
- W1999118410 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20423933" @default.
- W1999118410 hasPublicationYear "2010" @default.
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