FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999133031> ?p ?o ?g. }

• W1999133031 endingPage "369" @default.
• W1999133031 startingPage "359" @default.
• W1999133031 abstract "Different technologies have been employed to deliver the whole spectrum of tumor antigens (TAs) to dendritic cells (DCs) to be presented to T cells. These include whole tumor RNA-transfected DCs, preparations of DCs loaded with tumor-derived apoptotic bodies or tumor cell lysates, and DC tumor cell fusions. Early clinical trials have been conducted using such techniques. The presented study was aimed to revisit the necessity of tumor cell manipulation in DC-based immunotherapy strategies for colorectal carcinoma. We investigated a simple coculture method of autologous monocyte-derived DCs and human primary colorectal carcinoma (pCC) in comparison with 2 well-described cell fusion strategies for the efficacy of uptake, processing and presentation of TAs to autologous T cells. Before coculture or fusion, pCC had been cryopreserved without further manipulation. Fluorescence microscopy and flow cytometry analyses of fluorescent dye labeled cells were used for monitoring engulfment of pCC by DCs. The coculture procedure resulted in a double positive cell fraction of up to 22% and thus was comparable to that observed after cell fusion. More important, DCs after coculture with autologous pCC induced significant tumor-specific interferon-γ–producing autologous T cells in the same number of patients as DC/pCC fusions. Furthermore, tumor-specific major histocompatibility complex class I restricted cytotoxic T lymphocytes were generated by stimulation with DCs cocultured with pCC. In prior studies for human carcinomas coculture techniques were described to be inferior. In contrast, our data strongly suggest that at least for human pCC and autologous DCs this simple coculture method is similarly efficient compared to established fusion techniques." @default.
• W1999133031 created "2016-06-24" @default.
• W1999133031 creator A5017193823 @default.
• W1999133031 creator A5019504046 @default.
• W1999133031 creator A5025511599 @default.
• W1999133031 creator A5026928651 @default.
• W1999133031 creator A5040054777 @default.
• W1999133031 creator A5058511375 @default.
• W1999133031 creator A5059931392 @default.
• W1999133031 creator A5065105860 @default.
• W1999133031 creator A5082791032 @default.
• W1999133031 date "2007-05-01" @default.
• W1999133031 modified "2023-09-26" @default.
• W1999133031 title "Efficient Activation of Autologous Tumor-specific T Cells: A Simple Coculture Technique of Autologous Dendritic Cells Compared to Established Cell Fusion Strategies in Primary Human Colorectal Carcinoma" @default.
• W1999133031 cites W1493538138 @default.
• W1999133031 cites W1561591381 @default.
• W1999133031 cites W1587935721 @default.
• W1999133031 cites W1740181006 @default.
• W1999133031 cites W1927758176 @default.
• W1999133031 cites W1964037647 @default.
• W1999133031 cites W1964416857 @default.
• W1999133031 cites W1966335364 @default.
• W1999133031 cites W1967802864 @default.
• W1999133031 cites W1968668877 @default.
• W1999133031 cites W1969705214 @default.
• W1999133031 cites W1975559149 @default.
• W1999133031 cites W1988604479 @default.
• W1999133031 cites W2004217316 @default.
• W1999133031 cites W2010899054 @default.
• W1999133031 cites W2017591700 @default.
• W1999133031 cites W2022198469 @default.
• W1999133031 cites W2022308155 @default.
• W1999133031 cites W2023198916 @default.
• W1999133031 cites W2026364942 @default.
• W1999133031 cites W2027741075 @default.
• W1999133031 cites W2035298740 @default.
• W1999133031 cites W2035721245 @default.
• W1999133031 cites W2039394032 @default.
• W1999133031 cites W2048564277 @default.
• W1999133031 cites W2049895872 @default.
• W1999133031 cites W2052146292 @default.
• W1999133031 cites W2061366901 @default.
• W1999133031 cites W2064260736 @default.
• W1999133031 cites W2067489645 @default.
• W1999133031 cites W2070230314 @default.
• W1999133031 cites W2071074176 @default.
• W1999133031 cites W2073198208 @default.
• W1999133031 cites W2074229115 @default.
• W1999133031 cites W2075012280 @default.
• W1999133031 cites W2090454492 @default.
• W1999133031 cites W2100446366 @default.
• W1999133031 cites W2102689053 @default.
• W1999133031 cites W2108728495 @default.
• W1999133031 cites W2116693509 @default.
• W1999133031 cites W2117216774 @default.
• W1999133031 cites W2129980000 @default.
• W1999133031 cites W2142504949 @default.
• W1999133031 cites W2154041075 @default.
• W1999133031 cites W2163443542 @default.
• W1999133031 cites W2166584906 @default.
• W1999133031 cites W2169700552 @default.
• W1999133031 cites W2246326369 @default.
• W1999133031 cites W4249070333 @default.
• W1999133031 cites W72842197 @default.
• W1999133031 cites W2008571844 @default.
• W1999133031 doi "https://doi.org/10.1097/cji.0b013e31802bfefe" @default.
• W1999133031 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17457211" @default.
• W1999133031 hasPublicationYear "2007" @default.
• W1999133031 type Work @default.
• W1999133031 sameAs 1999133031 @default.
• W1999133031 citedByCount "9" @default.
• W1999133031 countsByYear W19991330312012 @default.
• W1999133031 countsByYear W19991330312016 @default.
• W1999133031 countsByYear W19991330312018 @default.
• W1999133031 countsByYear W19991330312021 @default.
• W1999133031 crossrefType "journal-article" @default.
• W1999133031 hasAuthorship W1999133031A5017193823 @default.
• W1999133031 hasAuthorship W1999133031A5019504046 @default.
• W1999133031 hasAuthorship W1999133031A5025511599 @default.
• W1999133031 hasAuthorship W1999133031A5026928651 @default.
• W1999133031 hasAuthorship W1999133031A5040054777 @default.
• W1999133031 hasAuthorship W1999133031A5058511375 @default.
• W1999133031 hasAuthorship W1999133031A5059931392 @default.
• W1999133031 hasAuthorship W1999133031A5065105860 @default.
• W1999133031 hasAuthorship W1999133031A5082791032 @default.
• W1999133031 hasConcept C147483822 @default.
• W1999133031 hasConcept C154317977 @default.
• W1999133031 hasConcept C185592680 @default.
• W1999133031 hasConcept C202751555 @default.
• W1999133031 hasConcept C203014093 @default.
• W1999133031 hasConcept C2776090121 @default.
• W1999133031 hasConcept C2777701055 @default.
• W1999133031 hasConcept C2778170410 @default.
• W1999133031 hasConcept C502942594 @default.
• W1999133031 hasConcept C553184892 @default.
• W1999133031 hasConcept C55493867 @default.
• W1999133031 hasConcept C71924100 @default.
• W1999133031 hasConcept C8891405 @default.

• next