FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999160425> ?p ?o ?g. }

• W1999160425 endingPage "83" @default.
• W1999160425 startingPage "75" @default.
• W1999160425 abstract "G-proteins have been implicated in the transduction of a number of flow-induced responses. We determined whether flow can modulate vascular endothelial or smooth muscle cell G-protein signaling. By use of a perfused transcapillary coculture system that permits the chronic exposure of cultured endothelial cells (ECs) and smooth muscle cells (SMCs) to physiological shear stresses, cocultures were exposed to stepwise increases in flow up to (1) 2 mL/min (low flow: 0.5 dyne/cm2), or (2) 44 mL/min (high flow: 15 dyne/cm2) and maintained for 72 hours before SMCs and ECs were harvested separately. Using Western blot analysis, EC Gi alpha3 expression was significantly increased (41+/-2.9%) by high-flow conditions compared with low-flow. The changes in G-protein expression were associated with a significant increase in endothelial nitric oxide synthase (eNOS) activity, elevated prostacyclin levels in the perfusing media, increased pertussis toxin-catalyzed ADP ribosylation of Gi alpha substrates, and enhanced agonist-stimulated GTPase activity in cocultured ECs. In contrast, high flow induced a significant decrease in Gi alpha1-2 expression (57 5%) in SMCs cocultured with ECs, an effect that was endothelium dependent, inhibited by indomethacin, and correlated with a decrease in pertussis toxin-catalyzed ADP ribosylation of Gi alpha substrates, reduced agonist-stimulated GTPase activity, and enhanced basal and G-protein-stimulated adenylyl cyclase activity. These data demonstrate that flow mediates selective changes in EC and SMC G-protein expression concomitant with changes in G-protein functionality and cellular signaling capacity. Moreover, flow-induced changes in SMC G-protein signaling capacity are endothelium dependent and require a cyclooxygenase product. G-protein modulation may thus represent an important mechanism whereby hemodynamic forces regulate vessel wall function." @default.
• W1999160425 created "2016-06-24" @default.
• W1999160425 creator A5019656476 @default.
• W1999160425 creator A5075291075 @default.
• W1999160425 creator A5085412846 @default.
• W1999160425 date "1998-01-01" @default.
• W1999160425 modified "2023-09-27" @default.
• W1999160425 title "Flow-Mediated Regulation of G-Protein Expression in Cocultured Vascular Smooth Muscle and Endothelial Cells" @default.
• W1999160425 cites W1491671746 @default.
• W1999160425 cites W1529148196 @default.
• W1999160425 cites W1924909341 @default.
• W1999160425 cites W1936770188 @default.
• W1999160425 cites W1969716556 @default.
• W1999160425 cites W1969812283 @default.
• W1999160425 cites W1999083451 @default.
• W1999160425 cites W2024741618 @default.
• W1999160425 cites W2026013318 @default.
• W1999160425 cites W2031805948 @default.
• W1999160425 cites W2032299053 @default.
• W1999160425 cites W2050728340 @default.
• W1999160425 cites W2051292594 @default.
• W1999160425 cites W2055846085 @default.
• W1999160425 cites W2059379142 @default.
• W1999160425 cites W2060984484 @default.
• W1999160425 cites W2062969839 @default.
• W1999160425 cites W2064255199 @default.
• W1999160425 cites W2065440690 @default.
• W1999160425 cites W2066646723 @default.
• W1999160425 cites W2067382519 @default.
• W1999160425 cites W2075108086 @default.
• W1999160425 cites W2079349364 @default.
• W1999160425 cites W2087388405 @default.
• W1999160425 cites W2090211483 @default.
• W1999160425 cites W2090255969 @default.
• W1999160425 cites W2121863292 @default.
• W1999160425 cites W2150775643 @default.
• W1999160425 cites W2153966044 @default.
• W1999160425 cites W2158592093 @default.
• W1999160425 cites W2414392119 @default.
• W1999160425 cites W4293247451 @default.
• W1999160425 doi "https://doi.org/10.1161/01.atv.18.1.75" @default.
• W1999160425 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9445259" @default.
• W1999160425 hasPublicationYear "1998" @default.
• W1999160425 type Work @default.
• W1999160425 sameAs 1999160425 @default.
• W1999160425 citedByCount "33" @default.
• W1999160425 countsByYear W19991604252012 @default.
• W1999160425 countsByYear W19991604252013 @default.
• W1999160425 countsByYear W19991604252016 @default.
• W1999160425 countsByYear W19991604252019 @default.
• W1999160425 countsByYear W19991604252022 @default.
• W1999160425 countsByYear W19991604252023 @default.
• W1999160425 crossrefType "journal-article" @default.
• W1999160425 hasAuthorship W1999160425A5019656476 @default.
• W1999160425 hasAuthorship W1999160425A5075291075 @default.
• W1999160425 hasAuthorship W1999160425A5085412846 @default.
• W1999160425 hasBestOaLocation W19991604251 @default.
• W1999160425 hasConcept C126322002 @default.
• W1999160425 hasConcept C134018914 @default.
• W1999160425 hasConcept C185592680 @default.
• W1999160425 hasConcept C202751555 @default.
• W1999160425 hasConcept C2776992346 @default.
• W1999160425 hasConcept C2777411675 @default.
• W1999160425 hasConcept C2777622882 @default.
• W1999160425 hasConcept C2778326061 @default.
• W1999160425 hasConcept C2779178603 @default.
• W1999160425 hasConcept C2779395532 @default.
• W1999160425 hasConcept C2781128415 @default.
• W1999160425 hasConcept C2992686903 @default.
• W1999160425 hasConcept C49773422 @default.
• W1999160425 hasConcept C519581460 @default.
• W1999160425 hasConcept C55493867 @default.
• W1999160425 hasConcept C62478195 @default.
• W1999160425 hasConcept C71924100 @default.
• W1999160425 hasConcept C80631254 @default.
• W1999160425 hasConcept C86803240 @default.
• W1999160425 hasConcept C95444343 @default.
• W1999160425 hasConceptScore W1999160425C126322002 @default.
• W1999160425 hasConceptScore W1999160425C134018914 @default.
• W1999160425 hasConceptScore W1999160425C185592680 @default.
• W1999160425 hasConceptScore W1999160425C202751555 @default.
• W1999160425 hasConceptScore W1999160425C2776992346 @default.
• W1999160425 hasConceptScore W1999160425C2777411675 @default.
• W1999160425 hasConceptScore W1999160425C2777622882 @default.
• W1999160425 hasConceptScore W1999160425C2778326061 @default.
• W1999160425 hasConceptScore W1999160425C2779178603 @default.
• W1999160425 hasConceptScore W1999160425C2779395532 @default.
• W1999160425 hasConceptScore W1999160425C2781128415 @default.
• W1999160425 hasConceptScore W1999160425C2992686903 @default.
• W1999160425 hasConceptScore W1999160425C49773422 @default.
• W1999160425 hasConceptScore W1999160425C519581460 @default.
• W1999160425 hasConceptScore W1999160425C55493867 @default.
• W1999160425 hasConceptScore W1999160425C62478195 @default.
• W1999160425 hasConceptScore W1999160425C71924100 @default.
• W1999160425 hasConceptScore W1999160425C80631254 @default.
• W1999160425 hasConceptScore W1999160425C86803240 @default.
• W1999160425 hasConceptScore W1999160425C95444343 @default.
• W1999160425 hasIssue "1" @default.

• next