Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999166546> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W1999166546 endingPage "8825" @default.
- W1999166546 startingPage "8819" @default.
- W1999166546 abstract "In the typical base recognition mode of the C2H2-type zinc finger, the amino acid residues at α-helical positions −1, 3, and 6 make a contact with the base in one strand (the primary strand), and the residue at position 2 interacts with the base in a complementary strand (the secondary strand). The N-terminal zinc finger of the three-zinc-finger domain of Sp1 has inherently a unique five-base-pair binding mode in which the guanine bases are recognized in both strands. To clarify the effect of the amino acid at position 2 on DNA binding affinity and base specificity, we have created a library of the mutants by the interconversion between serine and aspartic acid in the N-terminal zinc finger of Sp1 and recombinant variants of finger order. Gel mobility shift and methylation interference assays showed that the combination of arginine and serine at positions −1 and 2, respectively, provides a newly strong guanine contact in the secondary strand and a higher binding affinity than that of wild-type Sp1. Of special interest are the facts that the mutant with lysine and aspartic acid at positions −1 and 2 in the α helix predominantly recognizes the bases in the secondary strand and that its DNA binding affinity is higher than that of the wild-type. The aspartic acid or serine at position 2 independently contributes to the DNA binding affinity and base specificity. The present results provide useful information for the design of a novel zinc finger protein with priority for the bases in the secondary strand." @default.
- W1999166546 created "2016-06-24" @default.
- W1999166546 creator A5000780771 @default.
- W1999166546 creator A5009218478 @default.
- W1999166546 creator A5021037133 @default.
- W1999166546 creator A5025053166 @default.
- W1999166546 creator A5061066996 @default.
- W1999166546 date "2002-06-20" @default.
- W1999166546 modified "2023-10-17" @default.
- W1999166546 title "Interconversion between Serine and Aspartic Acid in the α Helix of the N-Terminal Zinc Finger of Sp1: Implication for General Recognition Code and for Design of Novel Zinc Finger Peptide Recognizing Complementary Strand" @default.
- W1999166546 cites W1978349636 @default.
- W1999166546 cites W1982558048 @default.
- W1999166546 cites W1991048125 @default.
- W1999166546 cites W2011198690 @default.
- W1999166546 cites W2042765757 @default.
- W1999166546 cites W286297709 @default.
- W1999166546 cites W4231570218 @default.
- W1999166546 doi "https://doi.org/10.1021/bi0256429" @default.
- W1999166546 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12102624" @default.
- W1999166546 hasPublicationYear "2002" @default.
- W1999166546 type Work @default.
- W1999166546 sameAs 1999166546 @default.
- W1999166546 citedByCount "7" @default.
- W1999166546 countsByYear W19991665462020 @default.
- W1999166546 crossrefType "journal-article" @default.
- W1999166546 hasAuthorship W1999166546A5000780771 @default.
- W1999166546 hasAuthorship W1999166546A5009218478 @default.
- W1999166546 hasAuthorship W1999166546A5021037133 @default.
- W1999166546 hasAuthorship W1999166546A5025053166 @default.
- W1999166546 hasAuthorship W1999166546A5061066996 @default.
- W1999166546 hasConcept C104317684 @default.
- W1999166546 hasConcept C119489563 @default.
- W1999166546 hasConcept C181199279 @default.
- W1999166546 hasConcept C185592680 @default.
- W1999166546 hasConcept C186055190 @default.
- W1999166546 hasConcept C24284526 @default.
- W1999166546 hasConcept C2776414213 @default.
- W1999166546 hasConcept C2778301229 @default.
- W1999166546 hasConcept C2779856020 @default.
- W1999166546 hasConcept C2781208047 @default.
- W1999166546 hasConcept C35802184 @default.
- W1999166546 hasConcept C512185932 @default.
- W1999166546 hasConcept C515207424 @default.
- W1999166546 hasConcept C5432534 @default.
- W1999166546 hasConcept C552990157 @default.
- W1999166546 hasConcept C55493867 @default.
- W1999166546 hasConcept C71240020 @default.
- W1999166546 hasConcept C82381507 @default.
- W1999166546 hasConcept C86339819 @default.
- W1999166546 hasConceptScore W1999166546C104317684 @default.
- W1999166546 hasConceptScore W1999166546C119489563 @default.
- W1999166546 hasConceptScore W1999166546C181199279 @default.
- W1999166546 hasConceptScore W1999166546C185592680 @default.
- W1999166546 hasConceptScore W1999166546C186055190 @default.
- W1999166546 hasConceptScore W1999166546C24284526 @default.
- W1999166546 hasConceptScore W1999166546C2776414213 @default.
- W1999166546 hasConceptScore W1999166546C2778301229 @default.
- W1999166546 hasConceptScore W1999166546C2779856020 @default.
- W1999166546 hasConceptScore W1999166546C2781208047 @default.
- W1999166546 hasConceptScore W1999166546C35802184 @default.
- W1999166546 hasConceptScore W1999166546C512185932 @default.
- W1999166546 hasConceptScore W1999166546C515207424 @default.
- W1999166546 hasConceptScore W1999166546C5432534 @default.
- W1999166546 hasConceptScore W1999166546C552990157 @default.
- W1999166546 hasConceptScore W1999166546C55493867 @default.
- W1999166546 hasConceptScore W1999166546C71240020 @default.
- W1999166546 hasConceptScore W1999166546C82381507 @default.
- W1999166546 hasConceptScore W1999166546C86339819 @default.
- W1999166546 hasIssue "28" @default.
- W1999166546 hasLocation W19991665461 @default.
- W1999166546 hasLocation W19991665462 @default.
- W1999166546 hasOpenAccess W1999166546 @default.
- W1999166546 hasPrimaryLocation W19991665461 @default.
- W1999166546 hasRelatedWork W1485670237 @default.
- W1999166546 hasRelatedWork W2020895837 @default.
- W1999166546 hasRelatedWork W2027881262 @default.
- W1999166546 hasRelatedWork W2081033389 @default.
- W1999166546 hasRelatedWork W2127549001 @default.
- W1999166546 hasRelatedWork W2129237258 @default.
- W1999166546 hasRelatedWork W2381207891 @default.
- W1999166546 hasRelatedWork W2383779535 @default.
- W1999166546 hasRelatedWork W597440835 @default.
- W1999166546 hasRelatedWork W2468055366 @default.
- W1999166546 hasVolume "41" @default.
- W1999166546 isParatext "false" @default.
- W1999166546 isRetracted "false" @default.
- W1999166546 magId "1999166546" @default.
- W1999166546 workType "article" @default.