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- W1999201118 abstract "Capillary electrophoresis (CE) with indirect photometric detection (IPD) is being investigated in this laboratory as a complementary technique to reversed-phase ion chromatography (RPIC) for the separation and identification of mono- and diesters of C1C6 phosphates. Separation efficiency, resolution and analysis time were examined by comparing CE with RPIC using suitable indirect photometric reagents such as adenosine monophosphate (AMP) for CE and napthalene trisulfonate (NTS) for RPIC. A CE optimization study indicated that a baseline separation of ten mono- and diester C1C6 phosphates was possible in 10 min using 5 mM AMP-100 mM H3BO3 (pH 7.2). The separation of the same mixture with isocratic RPIC required two different elution conditions. A mixture of eight mono- and diester C1C4 phosphates required a 5% acetonitrile (ACN)-20 μM NTS mobile phase for separation in 24 min. The remaining two organophosphates, monoethylhexyl and diethylhexyl phosphate, needed 16 min for separation using an 85% ACN-10 μM NTS eluent. The use of diethylenetriamine as an electroosmotic flow suppressor increased the migration time but significantly improved the CE resolution of both aliphatic and aromatic ethoxylated phosphates. The results show that CE is a useful technique for the fingerprint analysis of commercial organophosphate surfactants such as branched- and straight-chain phosphates and polyphosphates." @default.
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- W1999201118 date "1996-06-01" @default.
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- W1999201118 title "Capillary electrophoresis of phosphate ester surfactants with adenosine monophosphate and indirect photometric detection" @default.
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- W1999201118 doi "https://doi.org/10.1016/0021-9673(95)01379-2" @default.
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