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- W1999247756 abstract "Chronic opioids induce synaptic plasticity, a major neuronal adaptation. Astrocyte activation in synaptogenesis may play a critical role in opioid tolerance, withdrawal, and dependence. Thrombospondins 1 and 2 (TSP1/2) are astrocyte-secreted matricellular glycoproteins that promote neurite outgrowth as well as dendritic spine and synapse formation, all of which are inhibited by chronic μ opioids. In prior studies, we discovered that the mechanism of TSP1 regulation by μ opioids in astrocytes involves crosstalk between three different classes of receptors, μ opioid receptor, EGFR and TGFβR. Moreover, TGFβ1 stimulated TSP1 expression via EGFR and ERK/MAPK activation, indicating that EGFR is a signaling hub for opioid and TGFβ1 actions. Using various selective antagonists, and inhibitors, here we compared the mechanisms of chronic opioid regulation of TSP1/2 isoform expression in vivo and in immortalized rat cortical astrocytes. TSP1/2 release from astrocytes was also monitored. Acute and chronic μ opioids, morphine, and the prototypic μ ligand, DAMGO, modulated TSP2 protein levels. TSP2 but not TSP1 protein content was up-regulated by acute (3 h) morphine or DAMGO by an ERK/MAPK dependent mechanism. Paradoxically, TSP2 protein levels were altered neither by TGFβ1 nor by astrocytic neurotrophic factors, EGF, CNTF, and BMP4. TSP1/2 immunofluorescence was increased in astrocytes subjected to scratch-wounding, suggesting TSPs may be useful markers for the reactive state of these cells and potentially for different types of injury. Previously, we determined that chronic morphine attenuated both neurite outgrowth and synapse formation in cocultures of primary astrocytes and neurons under similar temporal conditions that μ opioids reduced TSP1 protein levels in astrocytes. Here we found that, after the same 8 day treatment, morphine or DAMGO diminished TSP2 protein levels in astrocytes. Therefore, μ opioids may deter synaptogenesis via both TSP1/2 isoforms, but by distinct mechanisms." @default.
- W1999247756 created "2016-06-24" @default.
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- W1999247756 date "2013-12-05" @default.
- W1999247756 modified "2023-10-14" @default.
- W1999247756 title "Acute and Chronic Mu Opioids Differentially Regulate Thrombospondins 1 and 2 Isoforms in Astrocytes" @default.
- W1999247756 cites W111650051 @default.
- W1999247756 cites W1487872499 @default.
- W1999247756 cites W1964628210 @default.
- W1999247756 cites W1968789665 @default.
- W1999247756 cites W1969572743 @default.
- W1999247756 cites W1969688763 @default.
- W1999247756 cites W1970437830 @default.
- W1999247756 cites W1972304129 @default.
- W1999247756 cites W1974998836 @default.
- W1999247756 cites W1974999182 @default.
- W1999247756 cites W1975483277 @default.
- W1999247756 cites W1978712311 @default.
- W1999247756 cites W1979365023 @default.
- W1999247756 cites W1988452935 @default.
- W1999247756 cites W1988896202 @default.
- W1999247756 cites W1989766945 @default.
- W1999247756 cites W2008078163 @default.
- W1999247756 cites W2012935976 @default.
- W1999247756 cites W2017518987 @default.
- W1999247756 cites W2017523469 @default.
- W1999247756 cites W2018739518 @default.
- W1999247756 cites W2020657920 @default.
- W1999247756 cites W2021788634 @default.
- W1999247756 cites W2022149149 @default.
- W1999247756 cites W2023669293 @default.
- W1999247756 cites W2026807633 @default.
- W1999247756 cites W2027948664 @default.
- W1999247756 cites W2028601086 @default.
- W1999247756 cites W2029640500 @default.
- W1999247756 cites W2031611126 @default.
- W1999247756 cites W2031766979 @default.
- W1999247756 cites W2036755685 @default.
- W1999247756 cites W2038603249 @default.
- W1999247756 cites W2046571883 @default.
- W1999247756 cites W2047961059 @default.
- W1999247756 cites W2050420785 @default.
- W1999247756 cites W2051542435 @default.
- W1999247756 cites W2056986410 @default.
- W1999247756 cites W2066641421 @default.
- W1999247756 cites W2069498125 @default.
- W1999247756 cites W2073736976 @default.
- W1999247756 cites W2083830460 @default.
- W1999247756 cites W2084566588 @default.
- W1999247756 cites W2085424855 @default.
- W1999247756 cites W2086286771 @default.
- W1999247756 cites W2087749727 @default.
- W1999247756 cites W2089696270 @default.
- W1999247756 cites W2095213066 @default.
- W1999247756 cites W2098622411 @default.
- W1999247756 cites W2100526290 @default.
- W1999247756 cites W2101808673 @default.
- W1999247756 cites W2102355451 @default.
- W1999247756 cites W2104968232 @default.
- W1999247756 cites W2106165895 @default.
- W1999247756 cites W2106291035 @default.
- W1999247756 cites W2122281425 @default.
- W1999247756 cites W2123895632 @default.
- W1999247756 cites W2124940469 @default.
- W1999247756 cites W2127338217 @default.
- W1999247756 cites W21386982 @default.
- W1999247756 cites W2156298828 @default.
- W1999247756 cites W2326762586 @default.
- W1999247756 cites W2396597020 @default.
- W1999247756 cites W4211045489 @default.
- W1999247756 doi "https://doi.org/10.1021/cn400172n" @default.
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