FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999291224> ?p ?o ?g. }

• W1999291224 endingPage "1292" @default.
• W1999291224 startingPage "1281" @default.
• W1999291224 abstract "We established a novel technique for differential activity-based gel electrophoresis (DABGE) of lipolytic enzymes from two different biological samples. For this purpose, a set of three fluorescent suicide inhibitors was developed. These probes possess the same substrate analogous structures but carry different cyanine dyes (Cy2b, Cy3, and Cy5) as reporter fluorophores. For comparison of enzyme profiles, two samples are individually labeled with a different probe followed by mixing, gel electrophoresis, fluorescence imaging, and identification of the tagged proteins by MS/MS. Protocols for quantitative determination of active enzymes were developed on the basis of lipolytic proteomes that had been admixed with defined amounts of known lipases and esterases. A detailed analysis of the fluorescence intensities showed that the found enzyme ratios very closely reflected the relative amounts of the labeled enzymes that were used for spiking. The DABGE method was used to compare the lipolytic proteomes of brown and white adipose tissue showing specific enzyme patterns of both samples. This study represents the first application of this technology for comparative analysis of lipases and esterases. Further applications of this technique can be expected to provide entirely new information on lipid enzymology in health and disease with high precision." @default.
• W1999291224 created "2016-06-24" @default.
• W1999291224 creator A5017919644 @default.
• W1999291224 creator A5027513682 @default.
• W1999291224 creator A5035832516 @default.
• W1999291224 creator A5045035883 @default.
• W1999291224 creator A5057895676 @default.
• W1999291224 creator A5065889931 @default.
• W1999291224 creator A5083765016 @default.
• W1999291224 date "2009-07-01" @default.
• W1999291224 modified "2023-10-18" @default.
• W1999291224 title "Differential activity-based gel electrophoresis for comparative analysis of lipolytic and esterolytic activities" @default.
• W1999291224 cites W1606423922 @default.
• W1999291224 cites W1974545690 @default.
• W1999291224 cites W1979542564 @default.
• W1999291224 cites W1981499318 @default.
• W1999291224 cites W1982517443 @default.
• W1999291224 cites W2001076957 @default.
• W1999291224 cites W2005901495 @default.
• W1999291224 cites W2011037447 @default.
• W1999291224 cites W2012493662 @default.
• W1999291224 cites W2017052574 @default.
• W1999291224 cites W2023935690 @default.
• W1999291224 cites W2046176730 @default.
• W1999291224 cites W2048333869 @default.
• W1999291224 cites W2048358490 @default.
• W1999291224 cites W2050499789 @default.
• W1999291224 cites W2065636587 @default.
• W1999291224 cites W2068989602 @default.
• W1999291224 cites W2069682831 @default.
• W1999291224 cites W2070511522 @default.
• W1999291224 cites W2076515797 @default.
• W1999291224 cites W2078452116 @default.
• W1999291224 cites W2078594804 @default.
• W1999291224 cites W2092924155 @default.
• W1999291224 cites W2110603815 @default.
• W1999291224 cites W2113761630 @default.
• W1999291224 cites W2133009318 @default.
• W1999291224 cites W2143323618 @default.
• W1999291224 cites W2166998713 @default.
• W1999291224 cites W2171257957 @default.
• W1999291224 cites W4293247451 @default.
• W1999291224 doi "https://doi.org/10.1194/jlr.m800566-jlr200" @default.
• W1999291224 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2694328" @default.
• W1999291224 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19282273" @default.
• W1999291224 hasPublicationYear "2009" @default.
• W1999291224 type Work @default.
• W1999291224 sameAs 1999291224 @default.
• W1999291224 citedByCount "19" @default.
• W1999291224 countsByYear W19992912242012 @default.
• W1999291224 countsByYear W19992912242013 @default.
• W1999291224 countsByYear W19992912242017 @default.
• W1999291224 countsByYear W19992912242022 @default.
• W1999291224 countsByYear W19992912242023 @default.
• W1999291224 crossrefType "journal-article" @default.
• W1999291224 hasAuthorship W1999291224A5017919644 @default.
• W1999291224 hasAuthorship W1999291224A5027513682 @default.
• W1999291224 hasAuthorship W1999291224A5035832516 @default.
• W1999291224 hasAuthorship W1999291224A5045035883 @default.
• W1999291224 hasAuthorship W1999291224A5057895676 @default.
• W1999291224 hasAuthorship W1999291224A5065889931 @default.
• W1999291224 hasAuthorship W1999291224A5083765016 @default.
• W1999291224 hasBestOaLocation W19992912241 @default.
• W1999291224 hasConcept C104397665 @default.
• W1999291224 hasConcept C116084860 @default.
• W1999291224 hasConcept C121332964 @default.
• W1999291224 hasConcept C181199279 @default.
• W1999291224 hasConcept C185592680 @default.
• W1999291224 hasConcept C18903297 @default.
• W1999291224 hasConcept C2777289219 @default.
• W1999291224 hasConcept C2777324032 @default.
• W1999291224 hasConcept C2779697368 @default.
• W1999291224 hasConcept C40250595 @default.
• W1999291224 hasConcept C43617362 @default.
• W1999291224 hasConcept C55493867 @default.
• W1999291224 hasConcept C62520636 @default.
• W1999291224 hasConcept C86803240 @default.
• W1999291224 hasConcept C91881484 @default.
• W1999291224 hasConceptScore W1999291224C104397665 @default.
• W1999291224 hasConceptScore W1999291224C116084860 @default.
• W1999291224 hasConceptScore W1999291224C121332964 @default.
• W1999291224 hasConceptScore W1999291224C181199279 @default.
• W1999291224 hasConceptScore W1999291224C185592680 @default.
• W1999291224 hasConceptScore W1999291224C18903297 @default.
• W1999291224 hasConceptScore W1999291224C2777289219 @default.
• W1999291224 hasConceptScore W1999291224C2777324032 @default.
• W1999291224 hasConceptScore W1999291224C2779697368 @default.
• W1999291224 hasConceptScore W1999291224C40250595 @default.
• W1999291224 hasConceptScore W1999291224C43617362 @default.
• W1999291224 hasConceptScore W1999291224C55493867 @default.
• W1999291224 hasConceptScore W1999291224C62520636 @default.
• W1999291224 hasConceptScore W1999291224C86803240 @default.
• W1999291224 hasConceptScore W1999291224C91881484 @default.
• W1999291224 hasIssue "7" @default.
• W1999291224 hasLocation W19992912241 @default.
• W1999291224 hasLocation W19992912242 @default.
• W1999291224 hasLocation W19992912243 @default.
• W1999291224 hasLocation W19992912244 @default.

• next