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• W1999291310 endingPage "424" @default.
• W1999291310 startingPage "418" @default.
• W1999291310 abstract "This study focused on the enzymatic biotransformation of the major ginsenoside Rb1 into Rd for the mass production of minor ginsenosides using a novel recombinant <TEX>${beta}$</TEX>-glucosidase from Flavobacterium johnsoniae. The gene (bglF3) consisting of 2,235 bp (744 amino acid residues) was cloned and the recombinant enzyme overexpressed in Escherichia coli BL21(DE3) was characterized. This enzyme could transform ginsenoside Rb1 and gypenoside XVII to the ginsenosides Rd and F2, respectively. The glutathione S-transferase (GST) fused BglF3 was purified with GST-bind agarose resin and characterized. The kinetic parameters for <TEX>${beta}$</TEX>-glucosidase had apparent <TEX>$K_m$</TEX> values of <TEX>$0.91{pm}0.02$</TEX> and <TEX>$2.84{pm}0.05$</TEX> mM and <TEX>$V_{max}$</TEX> values of <TEX>$5.75{pm}0.12$</TEX> and <TEX>$0.71{pm}0.01{mu}mol{cdot}min^{-1}{cdot}mg$</TEX> of <TEX>$protein^{-1}$</TEX> against p-nitrophenyl-<TEX>${beta}$</TEX>-D-glucopyranoside and Rb1, respectively. At optimal conditions of pH 6.0 and <TEX>$37^{circ}C$</TEX>, BglF3 could only hydrolyze the outer glucose moiety of ginsenoside Rb1 and gypenoside XVII at the C-20 position of aglycon into ginsenosides Rd and F2, respectively. These results indicate that the recombinant BglF3 could be useful for the mass production of ginsenosides Rd and F2 in the pharmaceutical or cosmetic industry." @default.
• W1999291310 created "2016-06-24" @default.
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• W1999291310 date "2012-10-15" @default.
• W1999291310 modified "2023-10-04" @default.
• W1999291310 title "Enzymatic Biotransformation of Ginsenoside Rb1 and Gypenoside XVII into Ginsenosides Rd and F2 by Recombinant β-glucosidase from Flavobacterium johnsoniae" @default.
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• W1999291310 doi "https://doi.org/10.5142/jgr.2012.36.4.418" @default.
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