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- W1999295317 abstract "Abstract A study of the recovery process of Bacillus thuringiensis ( Bt ) strain BLB1-based bioinsecticides using ultrafiltration showed that the highest recovery of BLB1 bioactive components was achieved by using a 5 kDa MWCO ultrafiltration membrane to concentrate the supernatant. A bioassay against Ephestia kuehniella showed that the retentate was approximately 49% more toxic than the supernatant, with a lethal concentration (LC 50 ) of 194.00 μg g −1 in the retentate compared to 380.00 μg g −1 in the supernatant. Furthermore, compared to the centrifugate (spore-crystal mixture) mixed with supernatant or saline water, the centrifugate retentate showed 42.7% and 56% higher toxicity against E. kuehniella , respectively. The involvement of proteases, chitinases and Vip3 proteins restored to the retentate suggests synergetic activity with delta-endotoxins and spores, and this activity was confirmed by enzymatic activity and western blot analysis. The formulated centrifugate retentate maintained stability and toxicity after 1 year of storage at 4 °C. These data suggest that the ultrafiltration process enabled significant virulence factors recovery from BLB1 supernatant grown in a complex medium and resulted in a highly toxic and stable formulation." @default.
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- W1999295317 date "2014-11-01" @default.
- W1999295317 modified "2023-10-14" @default.
- W1999295317 title "Impact of liquid formulation based on ultrafiltration-recovered bioactive components on toxicity of Bacillus thuringiensis subsp. kurstaki strain BLB1 against Ephestia kuehniella" @default.
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- W1999295317 doi "https://doi.org/10.1016/j.procbio.2014.07.024" @default.
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