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Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999311301> ?p ?o ?g. }

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• W1999311301 abstract "After complete cleavage of ricin interchain disulfide bridge by 0.05 M dithiothreitol in nondenaturing conditions at 37 degrees C during 1 h 30 min, A- and B-chains were separated on a lactosaminyl-aminoethyl Biogel P-150 column at 4 degrees C, in the presence of 0.01 M dithiothreitol and 0.5 M MgCl2. A- and B-chains have been characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunology. Their specific activities have been tested by protein synthesis inhibition in a cell-free assay (rabbit reticulocyte lysate) and on whole cells (Zajdela hepatoma cells) and by hemagglutination. From these tests, the apparent cross contamination of the chains was about 0.1%." @default.
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• W1999311301 date "1988-08-01" @default.
• W1999311301 modified "2023-09-25" @default.
• W1999311301 title "Separation of ricin A- and B-chains after dithiothreitol reduction" @default.
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• W1999311301 doi "https://doi.org/10.1016/0003-2697(88)90170-4" @default.
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