FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999320859> ?p ?o ?g. }

• W1999320859 endingPage "94" @default.
• W1999320859 startingPage "89" @default.
• W1999320859 abstract "Extracellular adenosine 5′-triphosphate (ATP)-evoked increases in intracellular calcium and the consequent stimulation of calcium-mediated protein phosphorylation systems were investigated in primary cultures of rat cerebral cortical astrocytes. Measurement of calcium responses in fura-2-loaded astrocytes indicated that extracellular ATP stimulated a transient calcium peak followed by a sustained increase in intracellular calcium which declined to baseline when external calcium was removed, thereby indicating that ATP evokes mobilization of internal calcium as well as influx of external calcium. Protein phosphorylation studies revealed that application of extracellular ATP resulted in increased phosphorylation of 55 and 52 kDa proteins (4-fold and 2-fold, respectively) and decreased phosphorylation of 24 and 21 kDa proteins (approximately 50% for each protein). These effects were time- and dose-dependent. The changes in phosphate incorporation were (a) inhibited by lanthanum, (b) reduced when calcium was omitted from the bath and (c) mimicked by ionomycin, thus suggesting that the ATP-induced changes in protein phosphorylation were dependent on increased levels of intracellular calcium. Adenosine diphosphate (ADP) gave similar, but reduced, effects while adenosine and guanosine triphosphate (GTP) were ineffective, findings consistent with activation of P2 purinergic receptors. The 52 kDa protein co-migrated with glial fibrillary acidic protein. These results support the premise that calcium-dependent protein kinases and phosphatases are transducing elements for the calcium signal brought about by activation of P2 purinergic receptors in astrocytes. Since ATP is released from neurons and endothelial cells, this signal transduction mechanism may be an important component of neuronal- and endothelial-astrocytic communication." @default.
• W1999320859 created "2016-06-24" @default.
• W1999320859 creator A5019198824 @default.
• W1999320859 creator A5036399835 @default.
• W1999320859 creator A5054202830 @default.
• W1999320859 creator A5062029524 @default.
• W1999320859 creator A5063320386 @default.
• W1999320859 creator A5080042314 @default.
• W1999320859 date "1991-12-01" @default.
• W1999320859 modified "2023-10-14" @default.
• W1999320859 title "ATP-evoked calcium signal stimulates protein phosphorylation/dephosphorylation in astrocytes" @default.
• W1999320859 cites W1198538403 @default.
• W1999320859 cites W1528206681 @default.
• W1999320859 cites W1546522923 @default.
• W1999320859 cites W1572201679 @default.
• W1999320859 cites W1821651100 @default.
• W1999320859 cites W1947888345 @default.
• W1999320859 cites W1969470553 @default.
• W1999320859 cites W1970082704 @default.
• W1999320859 cites W1978864769 @default.
• W1999320859 cites W1996661902 @default.
• W1999320859 cites W1997093700 @default.
• W1999320859 cites W2002228741 @default.
• W1999320859 cites W2004917770 @default.
• W1999320859 cites W2009935511 @default.
• W1999320859 cites W2022821313 @default.
• W1999320859 cites W2024578017 @default.
• W1999320859 cites W2030323825 @default.
• W1999320859 cites W2035971243 @default.
• W1999320859 cites W2050074458 @default.
• W1999320859 cites W2056836891 @default.
• W1999320859 cites W2062543403 @default.
• W1999320859 cites W2062553152 @default.
• W1999320859 cites W2068270309 @default.
• W1999320859 cites W2071489012 @default.
• W1999320859 cites W2071544522 @default.
• W1999320859 cites W2078733990 @default.
• W1999320859 cites W2081486880 @default.
• W1999320859 cites W2100837269 @default.
• W1999320859 cites W2141260882 @default.
• W1999320859 cites W2153762697 @default.
• W1999320859 cites W4361867828 @default.
• W1999320859 cites W2569854065 @default.
• W1999320859 doi "https://doi.org/10.1016/0006-8993(91)91684-s" @default.
• W1999320859 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1814559" @default.
• W1999320859 hasPublicationYear "1991" @default.
• W1999320859 type Work @default.
• W1999320859 sameAs 1999320859 @default.
• W1999320859 citedByCount "53" @default.
• W1999320859 countsByYear W19993208592013 @default.
• W1999320859 countsByYear W19993208592017 @default.
• W1999320859 countsByYear W19993208592018 @default.
• W1999320859 countsByYear W19993208592019 @default.
• W1999320859 countsByYear W19993208592020 @default.
• W1999320859 crossrefType "journal-article" @default.
• W1999320859 hasAuthorship W1999320859A5019198824 @default.
• W1999320859 hasAuthorship W1999320859A5036399835 @default.
• W1999320859 hasAuthorship W1999320859A5054202830 @default.
• W1999320859 hasAuthorship W1999320859A5062029524 @default.
• W1999320859 hasAuthorship W1999320859A5063320386 @default.
• W1999320859 hasAuthorship W1999320859A5080042314 @default.
• W1999320859 hasBestOaLocation W19993208591 @default.
• W1999320859 hasConcept C11960822 @default.
• W1999320859 hasConcept C178666793 @default.
• W1999320859 hasConcept C178790620 @default.
• W1999320859 hasConcept C181080969 @default.
• W1999320859 hasConcept C181912034 @default.
• W1999320859 hasConcept C185592680 @default.
• W1999320859 hasConcept C2776989580 @default.
• W1999320859 hasConcept C2779564974 @default.
• W1999320859 hasConcept C28406088 @default.
• W1999320859 hasConcept C519063684 @default.
• W1999320859 hasConcept C55493867 @default.
• W1999320859 hasConcept C57306754 @default.
• W1999320859 hasConcept C79879829 @default.
• W1999320859 hasConcept C86803240 @default.
• W1999320859 hasConcept C87325107 @default.
• W1999320859 hasConcept C95444343 @default.
• W1999320859 hasConcept C97029542 @default.
• W1999320859 hasConceptScore W1999320859C11960822 @default.
• W1999320859 hasConceptScore W1999320859C178666793 @default.
• W1999320859 hasConceptScore W1999320859C178790620 @default.
• W1999320859 hasConceptScore W1999320859C181080969 @default.
• W1999320859 hasConceptScore W1999320859C181912034 @default.
• W1999320859 hasConceptScore W1999320859C185592680 @default.
• W1999320859 hasConceptScore W1999320859C2776989580 @default.
• W1999320859 hasConceptScore W1999320859C2779564974 @default.
• W1999320859 hasConceptScore W1999320859C28406088 @default.
• W1999320859 hasConceptScore W1999320859C519063684 @default.
• W1999320859 hasConceptScore W1999320859C55493867 @default.
• W1999320859 hasConceptScore W1999320859C57306754 @default.
• W1999320859 hasConceptScore W1999320859C79879829 @default.
• W1999320859 hasConceptScore W1999320859C86803240 @default.
• W1999320859 hasConceptScore W1999320859C87325107 @default.
• W1999320859 hasConceptScore W1999320859C95444343 @default.
• W1999320859 hasConceptScore W1999320859C97029542 @default.
• W1999320859 hasIssue "1-2" @default.
• W1999320859 hasLocation W19993208591 @default.

• next