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- W1999377167 abstract "A keratin set which consists of a type I 47kd and a type II 57kd protein occurs as a major constituent of the keratin patterns of various Internal stratified epithelia of the mouse. We have isolated specific cDNA clones of the two complementary keratin subunits from a cDNA library constructed with polyA + RNA of mouse tongue epithelium and present the complete nucleo-t1de and deduced amino add sequences of the 57kd protein and about 75% of the corresponding data of the 47kd protein. The comparison of the sequence data with those of known epidermal keratin mRNAs coding for the two types of keratin proteins reveals a fundamentally identical and type-specific organization of the mRNAs into both highly conserved and variable domains. In order to avoid cross-reactions wtth other members of the keratin multigene family, appropriately taylored 35 S-labeled cDNA probes comprising the low and non-homologous 3′ coding and noncoding domains of the mRNAs were used for in situ hybridization to tissue sections. The localization and distribution of the corresponding transcripts indicates a strongly compartmentalized keratin expression in mouse tongue epithelium." @default.
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- W1999377167 date "1986-01-26" @default.
- W1999377167 modified "2023-10-16" @default.
- W1999377167 title "Nonepidermal members of the keratin multigene family: cDNA sequences and in situ localization of the mRNAs" @default.
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- W1999377167 doi "https://doi.org/10.1093/nar/14.2.751" @default.
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