Matches in SemOpenAlex for { <https://semopenalex.org/work/W1999381646> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W1999381646 endingPage "622" @default.
- W1999381646 startingPage "618" @default.
- W1999381646 abstract "In the present study the hepatitis B virus X antigen binding protein 1 (XBP1) was cloned by inducing its expression, and its subcellular localization and function were examined. Total RNA was extracted from HepG2 cells and XBP1 was amplified using reverse transcription polymerase chain reaction (RT-PCR), followed by restriction enzyme digestion of the pGBKT7 yeast plasmid and identification by enzyme digestion. The plasmid was transformed into AH109 yeast via the lithium acetate method and protein extracts were prepared. XBP1 protein expression in the eukaryotic cells was determined using polyacrylamide gel electrophoresis and western blot analysis. The gene encoding the XBP1-binding protein was screened in liver cells using yeast two-hybrid technology. We transfected a human hepatocellular carcinoma cell line and observed the intracellular localization of the gene expression protein using a fluorescence microscope, followed by prokaryotic expression and XBP1 gene identification. A 921-bp XBP1 gene fragment was obtained via RT-PCR amplification and 20 proteins with known functions that interact with XBP1 were screened, including metallothionein, smooth muscle cell-related protein, asialoglycoprotein receptor, pyruvate dehydrogenase kinase 1 and a sequence with unknown functions. A green fluorescent protein expression plasmid pEGFP-C1-XBP1 of XBP1 was constructed successfully and its expression protein was localized in the cytoplasm. A 56-kDa recombinant protein was successfully obtained via prokaryotic expression and was demonstrated to have good specificity using western blot analysis. The XBP1 gene, which expresses the XBP1 protein, is located in the cytoplasm and plays a role in the intracellular structure, cell growth, intracellular metabolism and signal transduction pathway, as well as DNA duplication, transcription, recombination and repair." @default.
- W1999381646 created "2016-06-24" @default.
- W1999381646 creator A5001730991 @default.
- W1999381646 creator A5008282602 @default.
- W1999381646 creator A5025115516 @default.
- W1999381646 creator A5046804232 @default.
- W1999381646 date "2012-12-13" @default.
- W1999381646 modified "2023-09-30" @default.
- W1999381646 title "Cloning and functions of the HBxAg-binding protein XBP1" @default.
- W1999381646 cites W1553210691 @default.
- W1999381646 cites W1839631757 @default.
- W1999381646 cites W1980008063 @default.
- W1999381646 cites W1988917342 @default.
- W1999381646 cites W1991104308 @default.
- W1999381646 cites W1998996199 @default.
- W1999381646 cites W2000324336 @default.
- W1999381646 cites W2021865450 @default.
- W1999381646 cites W2033973298 @default.
- W1999381646 cites W2036549271 @default.
- W1999381646 cites W2061565948 @default.
- W1999381646 cites W2071204436 @default.
- W1999381646 cites W2100798094 @default.
- W1999381646 cites W2104868487 @default.
- W1999381646 cites W2127076236 @default.
- W1999381646 cites W2132221280 @default.
- W1999381646 cites W2154011816 @default.
- W1999381646 cites W2164322519 @default.
- W1999381646 cites W2168783124 @default.
- W1999381646 cites W2395263571 @default.
- W1999381646 cites W349677435 @default.
- W1999381646 cites W876777524 @default.
- W1999381646 doi "https://doi.org/10.3892/mmr.2012.1232" @default.
- W1999381646 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23241634" @default.
- W1999381646 hasPublicationYear "2012" @default.
- W1999381646 type Work @default.
- W1999381646 sameAs 1999381646 @default.
- W1999381646 citedByCount "4" @default.
- W1999381646 countsByYear W19993816462018 @default.
- W1999381646 countsByYear W19993816462020 @default.
- W1999381646 countsByYear W19993816462023 @default.
- W1999381646 crossrefType "journal-article" @default.
- W1999381646 hasAuthorship W1999381646A5001730991 @default.
- W1999381646 hasAuthorship W1999381646A5008282602 @default.
- W1999381646 hasAuthorship W1999381646A5025115516 @default.
- W1999381646 hasAuthorship W1999381646A5046804232 @default.
- W1999381646 hasBestOaLocation W19993816461 @default.
- W1999381646 hasConcept C104317684 @default.
- W1999381646 hasConcept C153911025 @default.
- W1999381646 hasConcept C2781331208 @default.
- W1999381646 hasConcept C33161422 @default.
- W1999381646 hasConcept C40767141 @default.
- W1999381646 hasConcept C54458228 @default.
- W1999381646 hasConcept C55493867 @default.
- W1999381646 hasConcept C67705224 @default.
- W1999381646 hasConcept C86803240 @default.
- W1999381646 hasConceptScore W1999381646C104317684 @default.
- W1999381646 hasConceptScore W1999381646C153911025 @default.
- W1999381646 hasConceptScore W1999381646C2781331208 @default.
- W1999381646 hasConceptScore W1999381646C33161422 @default.
- W1999381646 hasConceptScore W1999381646C40767141 @default.
- W1999381646 hasConceptScore W1999381646C54458228 @default.
- W1999381646 hasConceptScore W1999381646C55493867 @default.
- W1999381646 hasConceptScore W1999381646C67705224 @default.
- W1999381646 hasConceptScore W1999381646C86803240 @default.
- W1999381646 hasIssue "2" @default.
- W1999381646 hasLocation W19993816461 @default.
- W1999381646 hasLocation W19993816462 @default.
- W1999381646 hasOpenAccess W1999381646 @default.
- W1999381646 hasPrimaryLocation W19993816461 @default.
- W1999381646 hasRelatedWork W1984583884 @default.
- W1999381646 hasRelatedWork W2354063377 @default.
- W1999381646 hasRelatedWork W2362401712 @default.
- W1999381646 hasRelatedWork W2363191578 @default.
- W1999381646 hasRelatedWork W2368658214 @default.
- W1999381646 hasRelatedWork W2376443560 @default.
- W1999381646 hasRelatedWork W2377905056 @default.
- W1999381646 hasRelatedWork W2383760163 @default.
- W1999381646 hasRelatedWork W2385288155 @default.
- W1999381646 hasRelatedWork W2390284118 @default.
- W1999381646 hasVolume "7" @default.
- W1999381646 isParatext "false" @default.
- W1999381646 isRetracted "false" @default.
- W1999381646 magId "1999381646" @default.
- W1999381646 workType "article" @default.